| 研究課題/領域番号 |
23K15608
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| 研究機関 | 弘前大学 |
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研究代表者 |
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| 研究期間 (年度) |
2023-04-01 – 2026-03-31
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| キーワード | biodosimetry / dicentric / radiation / c-banding / cytogentic / centromere |
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| 研究実績の概要 |
We have combined the conventional solid chromosome staining with a specialized technique that targets and marks the centromeric region using a proteolytic enzyme, acetic saline, and Giemsa. We have reduced the staining to be completed within just ninety minutes. New modifications also minimize chromosome expansion while preserving morphology. This improvement enhanced the identification of chromosomal aberrations. Preliminary results show a significant reduction in dicentric yield measurement disparities among scorers, with the coefficient of variation decreasing from 12% with conventional Giemsa staining to 1.2%. We have begun to modify the technique for use in other laboratories, such as those in South Korea and Singapore. Results have also been shared at international conferences.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
2: おおむね順調に進展している
理由
Our research is progressing smoothly in the laboratory. We have begun sharing our technique with other laboratories and at international conferences. However, we encountered an issue where some chemicals used in the experiment are available in Japan but not in other countries. To address this, we will collaborate with partner laboratories to modify the technique, ensuring it is accessible to all. We will also continue presenting our new staining method at international conferences and performing comparative analyses to validate its effectiveness.
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| 今後の研究の推進方策 |
We plan to continue with manuscript preparation and publish our findings as soon as possible. Following publication, we will conduct intercomparison exercises using the new technique. These exercises will validate the method's effectiveness in reducing variation in chromosomal aberration scoring. By comparing results across different laboratories, we aim to demonstrate the consistency and reliability of our technique, further establishing its usefulness in the scientific community. Additionally, we will share this at a large international conference in the U.S., which will allow other laboratories to use the published results.
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| 次年度使用額が生じた理由 |
We were unable to travel to Singapore as originally intended due to the ongoing progress of our experiments. As a result, we will use the remaining funds in the next fiscal year to complete these planned collaborations and further develop our research. Additionally, we plan to utilize the grant requested for the following fiscal year to continue our experimental work, enhance international cooperation, and disseminate our findings through publications and conferences.
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