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2023 年度 実績報告書

Genetic and Molecular Analysis of Macrophage Polarization in Medication-Related Osteonecrosis of the Jaw-like Lesions in mice

研究課題

研究課題/領域番号 23K16070
研究機関長崎大学

研究代表者

アルオマーリ ファラ  長崎大学, 医歯薬学総合研究科(歯学系), 特任研究員 (30951389)

研究期間 (年度) 2023-04-01 – 2024-03-31
キーワードMRONJ / macrophage / bisphosphonate / polarization
研究実績の概要

Medication-related osteonecrosis of the jaw (MRONJ) is a rare yet severe intractable disease with no definite treatment protocol. The aims of this study were: 1) to elucidate the different genetic and molecular pathways of macrophages involvement between BRONJ and DRONJ in murine models; 2) to clarify the connection of macrophage subtypes in the pathophysiology of MRONJ by performing multiple comprehensive analyses. An ultimate goal is to unravel the conjoined mechanisms of the immune and osseous systems to reach the basis of therapeutic strategies for MRONJ.
In this year, high-prevalence murine models of MRONJ-like lesions induced by combination administration of bisphosphonate or anti-RANKL antibody and cyclophosphamide with tooth extraction were created (BRONJ and DRONJ, respectively). Maxillae, spleen, sera, tibiae and femora were collected at euthanasia 2 weeks after tooth extraction. Micro computed tomography scan, hematoxylin & eosin staining, trichrome staining, TRAP staining, immunostaining such as blood and lymphatic vessels, CD38-positive M1 and CD163-positive M2 macrophages, and CD169-positive osteal macrophages, qPCR and ELISA were performed. Blood and lymphatic vessels were significantly reduced in BRONJ- but not DRONJ-like lesions. Polarization shifting of macrophages from M2 to M1 macrophages was significantly observed in both BRONJ- and DRONJ-like lesions. The number of CD169-positive osteal macrophages in bone marrow area was significantly changed.

  • 研究成果

    (1件)

すべて 2023

すべて 雑誌論文 (1件) (うち査読あり 1件)

  • [雑誌論文] Medication-related Osteonecrosis of the Jaw Induced by Regenerative Therapy in Implant Dentistry: A Scoping Review2023

    • 著者名/発表者名
      Al-Omari Farah A.、Kuroshima Shinichiro、Sawase Takashi
    • 雑誌名

      Journal of Dentistry

      巻: 138 ページ: 104682~104682

    • DOI

      10.1016/j.jdent.2023.104682

    • 査読あり

URL: 

公開日: 2024-12-25  

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