| 研究実績の概要 |
Firstly, 2 types of 3D cancer cell spheroids with round shape, high uniformity and cell viability, and long-time cultivability were successfully generated by bio-printer. And the size of the 3D cancer spheroids can be easily controlled by adjusting cell concentrations and bio-printer settings (needle size, extrusion pressures, and extrusion time).
Secondly, a 3D cancer cell spheroids focusing system was successfully constructed. The 3D cancer cell spheroids were flowed by a lamina flow and observed with two cameras in different directions to obtain the length and width of spheroids to provide the 3D shape informtion.
Finally, a self-made 3D shape analysis software was designed. The video taken during the spheroids flowing can be upload to the software, which can analyze the shape information of each spheroid automatically.
Therefore, the generation method of spheroids with different sizes and high throughput analysis system have been confirmed. The different size of spheroids will be used to evaluate the nano-medicine uptake profile. The high throughput analysis system will be applied to realize the high-throughput observation, which is planned as the goal of 2024-2025 in the proposed research.
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| 今後の研究の推進方策 |
Firstly, the light-sheet fluorescence microscopy will be constructed through using a cylinder lens to observe the inside of 3D cancer cell spheroids.
Secondly, the constructed light-sheet fluorescence microscopy will be integrated with the spheroids focusing system. Large amounts of 3D cancer cell spheroids will be focused by the spheroids focusing system. At the same time, the light-sheet fluorescence microscopy will capture the comprehensive information inside the 3D structure.
Finally, the 3D cancer cell spheroids will be treated with nano-medicine and observed with the constructed system to obtain the comprehensive dynamic behaviors of nanomedicine inside the 3D cancer cell spheroids.
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