研究実績の概要 |
Single-cell genomics has emerged as a revolutionary technology, transforming nearly every field of biomedical research. However, there is a growing recognition that cell populations harbor far more heterogeneity than expected, making bulk population analysis inadequate for fully characterizing their biological complexity. It is well established that heterogeneity is one of the hallmarks of human cancer cells. Accurately defining cell types and states and understanding how they arise in disease demands functional single-cell measurements. To make progress in this area, we need more specific functional single-cell pheno-genotyping. Like Atomic Force Microscopy (AFM), scanning ion conductance microscopy (SICM) is a type of non-contact scanning probe microscopy, which we have pioneered for living cell imaging and phenotyping. In addition to high-resolution topographical imaging, SICM can perform accurate cell stiffness measurements and map cell mechanical properties. In this study, we utilized SICM and its based multifunctional nanopipette to image phenotypes at the single-cell level and directly collect cytoplasm for genetic analysis. With this technology, we can investigate the phenotype and gene expression state of cancer cells, including the formation of a cancer cell-specific microenvironment, the generation of reactive oxygen species, and cell stiffness, which is closely related to cancer invasion. This approach will enhance our understanding of cancer development and progression at the single-cell level.
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現在までの達成度 (区分) |
現在までの達成度 (区分)
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理由
1. A cell’s mechanical properties have been linked to cancer development, motility and metastasis and are therefore an attractive target as a universal, scanning ion conductance microscopy (SICM) offers a nanoscale resolution, noncontact method of nanomechanical data acquisition for cell phenotyping. 2.We have established new methods with SICM and Pt-functionalized nanoelectrodes to measure dynamic extracellular to intracellular H2O2 gradients, cell morphology and stiffness of individual Caco-2 cells. 3. We report on single-molecule nanopore sensing combined with position-encoded DNA molecular probes, with chemistry tuned to simultaneously identify various antigen proteins and multiple RNA gene fragments of SARS-CoV-2 with high sensitivity and selectivity.
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