• 研究課題をさがす
  • 研究者をさがす
  • KAKENの使い方
  1. 課題ページに戻る

2023 年度 実績報告書

Plasma-modified peptides/proteins for multi-target anticancer treatment

研究課題

研究課題/領域番号 22H01212
配分区分補助金
研究機関九州大学

研究代表者

Attri Pankaj  九州大学, システム情報科学研究院, 学術研究員 (40868361)

研究分担者 田中 宏昌  名古屋大学, 低温プラズマ科学研究センター, 教授 (00508129)
栗田 弘史  豊橋技術科学大学, 工学(系)研究科(研究院), 准教授 (70512177)
竹内 希  東京工業大学, 工学院, 准教授 (80467018)
白谷 正治  九州大学, システム情報科学研究院, 教授 (90206293)
古閑 一憲  九州大学, システム情報科学研究院, 教授 (90315127)
研究期間 (年度) 2022-04-01 – 2027-03-31
キーワードBiomolecules / Computer Simulations / Plasma Chemistry / Fluorescence / Protein Binding
研究実績の概要

For the potential use of plasma in biomedical applications like cancer treatment, it is important to develop the plasma device and control reactive species generated, along with changes in pH and temperature of the solution. In Frontiers of Physics 2023, we analyzed how the concentration of ROS and RNS changes with feed gases like CO2 and N2. The formation of CO in CO2 plasma is the main key point between the two processes. Therefore, in another study published in Plasma Processes and Polymers 2024, we focused on the formation of CO in the gas phase. Additionally, to understand the dynamics of plasma streamers, we performed a 2D particle-in-cell/Monte Carlo collision model. This helps simulate the high-intensity discharge and streamer propagation better and work published in Plasma 2023. In Biomolecules 2023, we conducted molecular dynamics simulations to investigate the permeation capabilities of RONS across modified cell membranes. The simulation results showed that less hydrophilic species, i.e., NO, NO2, N2O4, and O3, have a higher penetration ability through nitro-oxidized PLB compared to hydrophilic RONS, i.e., HNO3, s-cis-HONO, s-trans-HONO, H2O2, HO2, and OH. In another study, we synthesized benzimidazole-2-ones and checked the anti-inflammatory and anti-cancer properties using computer simulation. In-silico docking studies demonstrated promising binding interactions with Heat Shock Protein 60 (HSP60), and molecular dynamics simulations supported these results. These results were published in Environmental Research 2024.

現在までの達成度 (区分)
現在までの達成度 (区分)

2: おおむね順調に進展している

理由

According to the project's work package, I have developed a new plasma device, a streamer plasma, that suits this study, as it can operate at low power and we can treat solutions in different environmental conditions. Secondly, I also developed the 0D and 1D models to detect the reactive species that can’t be detected in the liquids (short-lived reactive species), along with developing the 2D fluid model. Now, I am working on using a 2D fluid model with chemistry input. Further molecular dynamics simulation was also developed to study the cellular membrane for both oxidized and non-oxidized. I have also simulated the protein binding drug molecules to study the combined effect of protein and drug in future in-vitro studies. Currently, I simulated one heat shock protein HSP60, but later, I plan to simulate more proteins.

今後の研究の推進方策

To understand the nature of the induced changes in amino acid modification in a complex state. Further, we will use co-solvents like Ionic liquids to understand the effect of co-solvents on biomolecules in the presence of plasma analytical techniques like circular dichroism, fluorescence spectroscopy, and UV-visible spectroscopy, supported by computational methods. This will help mimic the cellular processes. In the biological system, many types of complicity can affect plasma action. Additionally, we will continuously analyze the possible changes in the protein structure by mimicking plasma oxidized amino acids and performing the Molecular dynamics simulations. Through this, we can know the possible structure changes and binding capacities of proteins that are hard to extract and check their binding with drugs.

  • 研究成果

    (21件)

すべて 2024 2023 その他

すべて 雑誌論文 (10件) (うち国際共著 10件、 査読あり 9件、 オープンアクセス 8件) 学会発表 (9件) (うち国際学会 9件、 招待講演 6件) 備考 (2件)

  • [雑誌論文] Plasma?ionic liquid-assisted CO<sub>2</sub> capture and conversion: A novel technology2024

    • 著者名/発表者名
      Attri Pankaj、Koga Kazunori、Razzokov Jamoliddin、Okumura Takamasa、Kamataki Kunihiro、Nozaki Tomohiro、Shiratani Masaharu
    • 雑誌名

      Applied Physics Express

      巻: 17 ページ: 046001~046001

    • DOI

      10.35848/1882-0786/ad33ea

    • 査読あり / オープンアクセス / 国際共著
  • [雑誌論文] Environmentally friendly and efficient TBHP-mediated catalytic reaction for the synthesis of substituted benzimidazole-2-ones: approach to pharmaceutical applications2024

    • 著者名/発表者名
      Indira Meeniga、Surendranath Reddy E.C.、Kamala Prasad Vasikarla、Satyanarayana Swamy Vyshnava、Kakarla Raghava Reddy、Venkata Krishna Reddy Motakatla、Attiri Pankaj、Vasu Govardhana Reddy Peddiahgari、Aminabhavi Tejraj M.
    • 雑誌名

      Environmental Research

      巻: 252 ページ: 118760~118760

    • DOI

      10.1016/j.envres.2024.118760

    • 査読あり / 国際共著
  • [雑誌論文] Growth control of Marchantia polymorpha gemmae using nonthermal plasma irradiation2024

    • 著者名/発表者名
      Tsuboyama Shoko、Okumura Takamasa、Attri Pankaj、Koga Kazunori、Shiratani Masaharu、Kuchitsu Kazuyuki
    • 雑誌名

      Scientific Reports

      巻: 14 ページ: 3172

    • DOI

      10.1038/s41598-024-53104-1

    • 査読あり / オープンアクセス / 国際共著
  • [雑誌論文] Influence of humidity on the plasma‐assisted CO<sub>2</sub> conversion2023

    • 著者名/発表者名
      Attri Pankaj、Okumura Takamasa、Takeuchi Nozomi、Kamataki Kunihiro、Koga Kazunori、Shiratani Masaharu
    • 雑誌名

      Plasma Processes and Polymers

      巻: 21 ページ: e2300141

    • DOI

      10.1002/ppap.202300141

    • 査読あり / 国際共著
  • [雑誌論文] Plasma-assisted CO2 and N2 conversion to plant nutrient2023

    • 著者名/発表者名
      Attri Pankaj、Okumura Takamasa、Takeuchi Nozomi、Kamataki Kunihiro、Koga Kazunori、Shiratani Masaharu
    • 雑誌名

      Frontiers in Physics

      巻: 11 ページ: 1211166

    • DOI

      10.3389/fphy.2023.1211166

    • 査読あり / オープンアクセス / 国際共著
  • [雑誌論文] Unraveling the Transport Properties of RONS across Nitro-Oxidized Membranes2023

    • 著者名/発表者名
      Abduvokhidov Davronjon、Yusupov Maksudbek、Shahzad Aamir、Attri Pankaj、Shiratani Masaharu、Oliveira Maria C.、Razzokov Jamoliddin
    • 雑誌名

      Biomolecules

      巻: 13 ページ: 1043~1043

    • DOI

      10.3390/biom13071043

    • 査読あり / オープンアクセス / 国際共著
  • [雑誌論文] Investigation of Plasma Propagation in Packed-Bed Dielectric Barrier Discharge Based on a Customized Particle-in-Cell/Monte Carlo Collision Model2023

    • 著者名/発表者名
      Li Xufeng、Zhang Leiyu、Shahzad Aamir、Attri Pankaj、Zhang Quanzhi
    • 雑誌名

      Plasma

      巻: 6 ページ: 637~648

    • DOI

      10.3390/plasma6040044

    • 査読あり / オープンアクセス / 国際共著
  • [雑誌論文] Reaction kinetics studies for phenol degradation under the impact of different gas bubbles and pH using gas?liquid discharge plasma2023

    • 著者名/発表者名
      El-Tayeb A.、Okumura Takamasa、Attri Pankaj、Kamataki Kunihiro、Koga Kazunori、Shiratani Masaharu
    • 雑誌名

      Japanese Journal of Applied Physics

      巻: 62 ページ: SN1010~SN1010

    • DOI

      10.35848/1347-4065/acebfb

    • 査読あり / オープンアクセス / 国際共著
  • [雑誌論文] Health assessment of rice cultivated and harvested from plasma-irradiated seeds2023

    • 著者名/発表者名
      Okumura Takamasa、Tanaka Hayate、Nakao Takumi、Anan Teruki、Arita Ryo、Shiraki Masaki、Shiraki Kayo、Miyabe Tomoyuki、Yamashita Daisuke、Matsuo Kayo、Attri Pankaj、Kamataki Kunihiro、Yamashita Naoto、Itagaki Naho、Shiratani Masaharu、Hosoda Satoshi、Tanaka Akiyo、Ishibashi Yushi、Koga Kazunori
    • 雑誌名

      Scientific Reports

      巻: 13 ページ: 17450

    • DOI

      10.1038/s41598-023-43897-y

    • オープンアクセス / 国際共著
  • [雑誌論文] Role of Direct Plasma Irradiation, Plasma-Activated Liquid, and Plasma-Treated Soil in Plasma Agriculture2023

    • 著者名/発表者名
      Attri Pankaj、Okumura Takamasa、Takeuchi Nozomi、Razzokov Jamoliddin、Zhang Quanzhi、Kamataki Kunihiro、Shiratani Masaharu、Koga Kazunori
    • 雑誌名

      Plasma Medicine

      巻: 13 ページ: 33~52

    • DOI

      10.1615/PlasmaMed.2023050454

    • 査読あり / オープンアクセス / 国際共著
  • [学会発表] Influence of CO and H2O2 in plasma agriculture2024

    • 著者名/発表者名
      Pankaj Attri
    • 学会等名
      16th International Symposium on Advanced Plasma Science
    • 国際学会
  • [学会発表] Reactive oxygen and nitrogen species effect on enzyme structure: Experimental and simulation studies2024

    • 著者名/発表者名
      Pankaj Attri
    • 学会等名
      International conference on chemical and biological sciences (ICCBS)
    • 国際学会 / 招待講演
  • [学会発表] New generation of plasma agriculture for sustainable farming2024

    • 著者名/発表者名
      Pankaj Attri
    • 学会等名
      The 5th International Conference on Radiation and Emission in Materials
    • 国際学会 / 招待講演
  • [学会発表] Effect of the plasma-generated reactive species on protein folding2023

    • 著者名/発表者名
      Pankaj Attri
    • 学会等名
      The 8th Asian Applied Physics Conference
    • 国際学会
  • [学会発表] Catalase enzyme inhibition's effect on plasma medicine2023

    • 著者名/発表者名
      Pankaj Attri
    • 学会等名
      7th Asia-Pacific Conference on Plasma Physics (AAPPS-DPP)
    • 国際学会 / 招待講演
  • [学会発表] Plasma effect on the enzyme structure: Experimental and simulation studies2023

    • 著者名/発表者名
      Pankaj Attri
    • 学会等名
      33th Annual Meeting of the MRS-Japan
    • 国際学会 / 招待講演
  • [学会発表] Plasma-assisted CO2 and N2 conversion into valuable products2023

    • 著者名/発表者名
      Pankaj Attri
    • 学会等名
      13th Asian-European International Conference on Plasma Surface Engineering (AEPSE 2023)
    • 国際学会 / 招待講演
  • [学会発表] Plasma-induced CO2 conversion: Experimental and Computational study2023

    • 著者名/発表者名
      Pankaj Attri
    • 学会等名
      25th International Symposium on Plasma Chemistry (ISPC25)
    • 国際学会
  • [学会発表] Atmospheric pressure plasma-induced CO2 conversion2023

    • 著者名/発表者名
      Pankaj Attri
    • 学会等名
      14th EU-Japan Joint Symposium on Plasma Processing (JSPP-14)
    • 国際学会 / 招待講演
  • [備考] Google Scholar

    • URL

      https://scholar.google.com/citations?user=LXH5lksAAAAJ&hl=en

  • [備考] Kyushu University and scopus

    • URL

      https://kyushu-u.pure.elsevier.com/ja/persons/pankaj-attri

URL: 

公開日: 2024-12-25  

サービス概要 検索マニュアル よくある質問 お知らせ 利用規程 科研費による研究の帰属

Powered by NII kakenhi