| 研究実績の概要 |
To understand how changes in haemodynamic forces affect the actin cytoskeleton in endothelial cells (ECs) in a living animal, we have established high resolution confocal imaging and analysis of actin organization in the zebrafish. With this methodology, we discovered that ECs generate different actin organization - circumferential, mesh and longitudinal - when subjected to normal levels of physiological flow. Upon increasing haemodynamic forces by increasing heart rate using isoprenaline, we observed an increase in mesh actin formation in ECs. We have also developed a 3D computational fluid dynamics model to calculate wall shear stress and luminal pressure that ECs are subjected.
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