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2022 年度 実績報告書

Thyroid hormones and Pigmentation pattern evolution in clownfish

研究課題

研究課題/領域番号 22H02678
配分区分補助金
研究機関沖縄科学技術大学院大学

研究代表者

LAUDET Vincent  沖縄科学技術大学院大学, 海洋生態進化発生生物学ユニット, 教授 (20898423)

研究分担者 木下 政人  京都大学, 農学研究科, 准教授 (60263125)
研究期間 (年度) 2022-04-01 – 2026-03-31
キーワードPigmentation / Clownfish / Thyroid Hormone / Ontogenesis
研究実績の概要

How differences in pigmentation arose have never been fully explained. In anemonefishes there is a difference in white bar formation between Amphiprion ocellaris and A. percula. As white bars are linked to thyroid hormones (TH) we study if this ontogeny difference is linked to differences in TH signaling. We collected samples of the 2 species from hatching until 100 days. We have added the closely related A. biaculeatus to determine the ancestral status of bar formation. The samples are ready and will soon be sent for Illumina sequencing. We treated A. ocellaris with thyroid hormone and to MPI (to block TH production) and we submitted the samples to transcriptomic analysis. We have established the CRISPR/Cas9 technology in A. ocellaris and have targeted a first gene (dio2).

現在までの達成度 (区分)
現在までの達成度 (区分)

4: 遅れている

理由

OIST anemonefish husbandry construction and start have been delayed due to Covid and minor technical issues. This is now mostly solved. We need the husbandry that is in the main campus as OIST marine station can only host native species and A. percula is coming from Australia. All the research about this species have been delayed as we had to establish collaboration with foreign labs to get samples of this species.

今後の研究の推進方策

1.We plan to check if the timing of the metamorphosis is similar in the two species using a comparative transcriptomic experiment. We have collected the samples of the 3 species (A. percula, A. ocellaris and A. biaculeatus) at various stages from hatching until the formation of second white bar in A. percula (100dph). The samples are ready and will soon be sent for Illumina sequencing.
2.We are analyzing the transcriptomic data of A. ocellaris treated with thyroid hormone and with MPI to block TH production to reveal the transcriptional cascade controlled by TH in this species.
3.With CRISPR/Cas9 technology Pr. Kinoshita lab have targeted a first gene in A. ocellaris (deiodinase 2). The mutant fish are growing. We will check the germline transmission of the mutation and analyze the phenotype.

  • 研究成果

    (4件)

すべて 2023 2022

すべて 雑誌論文 (3件) (うち国際共著 3件、 査読あり 1件、 オープンアクセス 3件) 図書 (1件)

  • [雑誌論文] Anemonefishes: A model system for evolutionary genomics2023

    • 著者名/発表者名
      Herrera Marcela、Ravasi Timothy、Laudet Vincent
    • 雑誌名

      F1000Research

      巻: 12 ページ: 204~204

    • DOI

      10.12688/f1000research.130752.1

    • 査読あり / オープンアクセス / 国際共著
  • [雑誌論文] The multi-level regulation of clownfish metamorphosis by thyroid hormones2022

    • 著者名/発表者名
      Roux Natacha、Miura Saori、Dussene M?lanie、Tara Yuki、Lee Fiona、de Bernard Simon、Reynaud Mathieu、Salis Pauline、Barua Agneesh、Boulahtouf Abdelhay、Balaguer Patrick、Gauthier Karine、Lecchini David、Gibert Yann、Besseau Laurence、Laudet Vincent
    • 雑誌名

      bioRxiv

      巻: n/a ページ: n/a

    • DOI

      10.1101/2022.03.04.482938

    • オープンアクセス / 国際共著
  • [雑誌論文] Megacolonies: an alternative social organization in anemonefishes?2022

    • 著者名/発表者名
      Mercader Manon、Zwahlen Jann、Hayashi Kina、Takamiyagi Hiroki、Tseng Yung-Che、Nguyen Hai-Thanh T.、Asada Keishu、Sowinski J?rome、Reimer James、Lecchini David、Laudet Vincent
    • 雑誌名

      bioRxiv

      巻: n/a ページ: n/a

    • DOI

      10.1101/2022.11.06.515354

    • オープンアクセス / 国際共著
  • [図書] Evolution, Development and Ecology of Anemonefishes. Model Organisms for Marine Science2022

    • 著者名/発表者名
      Laudet V and Ravasi T
    • 総ページ数
      306
    • 出版者
      CRC Press
    • ISBN
      978-0367645816

URL: 

公開日: 2023-12-25  

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