| 研究実績の概要 |
Sulforaphane (SFN) is a naturally occurring isothiocyanate, which is currently the topic of active research as a critical regulator of cellular defenses through the transcription factors. The protective effects are mediated transcriptional regulation of the Nrf2 and NFκB pathways. During exercise, SFN may improve endurance capacity by reducing oxidative stress. However, the evidence is still insufficient to make a practical insight of SFN consumption to improve exercise performance. The purpose of this study is to identify; the effect of SFN on exercise endurance capacity and exercise performance; the effect of SFN on muscle inflammation and oxidative stress; the signaling pathway that interferes with radical-mediated signals. In the first year, cell culture experiment was conducted.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
2: おおむね順調に進展している
理由
The purpose of this study was to identify the effect of SFN on LPS-induced inflammation and oxidative stress in C2C12 myoblasts and the related mechanisms. SFN up to 20 uM, LPS 1 ug/mL did not show any significant differences in cytotoxicity for 24 hours. C2C12 cells were cultured in growth medium with 10% fetal bovine serum. After reaching 70% confluence differentiation medium was used to produce myotubes, then added 10 uM and 20 uM of SFN and incubated for 24 hours, followed by incubation with LPS (0.5 ug/mL) for another 24 hours. Gene expression of IL-6, iNOS, NQO1, Nrf2, HO-1, myogenin, myoD was measured by PCR. LPS increased IL-6 and iNOS mRNA, but SFN-pretreated cells downregulated. SFN induced NQO1, HO-1, Nrf2, myogenin, myoD and low amplification was found in LPS-treated cells.
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| 今後の研究の推進方策 |
Until now results are very much satisfactory and further analyses are conducted to meet the purpose of this study.
After completing the cell culture experiments, human experimentation of SFN will be started. Based on the results of the cell culture experiment, manuscript will be submitted to any suitable journals. In the meantime, ethical permission for the human experimentation will be applied. A randomized double-blind placebo-controlled study will be conducted with SFN intervention among the exercise trained and untrained individuals. The protocol will be finalized once the ethical permission is granted. Based on the results of the experiment we will plan to write another manuscript and submit to the suitable journals.
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