| 現在までの達成度 (区分) |
現在までの達成度 (区分)
2: おおむね順調に進展している
理由
I have established an early-life stress (ELS) protocol and breeding of transgenic mice line and exposed half of the bred mice to ELS using limited bedding and nesting combined with maternal separation. I have weighed the control and stress mice on days 4,8,10,12,14,21,28,35 and 42 after birth to investigate weather ELS influences mice weights. Three types of behavioural experiments (open field, light-dark transition, elevated plus maze) have been conducted to measure whether ELS affects anxiety levels in adult mice. I have learnt and performed several craniotomy surgeries on the somatosensory cortex and neuron and astrocyte virus injections in adult mice. I have performed 2-photon microscopy for functional calcium imaging of astrocytes and neurons in awake mice.
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| 今後の研究の推進方策 |
Next, I will perform more craniotomy surgeries and virus injections for all breed transgenic ELS-exposed mice and control mice. In addition, all 40 mice will be subjected to behavioural tests. I am planning to measure plasma corticosterone levels from blood to determine the impacts of ELS on the levels of stress hormone. Additionally, I am planning to measure the restricted diffusion of water in brain tissue to determine structural information of brain tissue with ex vivo diffusion tensor imaging MRI technique from control and early-life stress exposed adult mice. In parallel, I will start analyzing the calcium imaging data.
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