研究課題
We have previously shown that it was difficult to evaluate 5 weeks CA-forming cells (CAFC) which is a surrogate indicator of hematopoietic stem cells because human stromal cells could not survive more than 4 weeks. In this study, we optimized surum free media, combination of cytokines and chemical agents such as stem cell factor, thrombopoietin, flt3 ligand with or without delta like protein 4 (DLL4) and GSK inhibitor. By using this media, bone marrow CD34+ cells could be cocultured for 8 weeks without disruption of the human stromal layer. Five week CAFC could be observed in all condition of cytokine combination with or without chemical inhibitor. However, because cytoplasmic appearance of cells in some CAs is quite irregular without DLL4 or GSK inhibitor, we conducted immunohistochemical staining. We found that CD34+CAFCs were exclusively observed in the presence of DLL4 or GSK inhibitor. Moreover, NOD/SCID repopulating cells were detected in the coculture containing CD34+CAFCs.
すべて 2014
すべて 雑誌論文 (2件) (うち査読あり 2件、 オープンアクセス 1件、 謝辞記載あり 1件) 学会発表 (1件)
Int J Hematol
巻: 99 ページ: 471-476
10.1007/s12185-014-1530-1
Case Rep Oncol
巻: 7 ページ: 692-699
10.1159/000368714