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2014 年度 実施状況報告書

魚類免疫機構における重要なサイトカインIL-2/15/15Lファミリーの解明

研究課題

研究課題/領域番号 25450310
研究機関藤田保健衛生大学

研究代表者

J・M Dijkstra  藤田保健衛生大学, 総合医科学研究所, 講師 (10387681)

研究期間 (年度) 2013-04-01 – 2016-03-31
キーワードIL-15-like / IL-15 / IL-2 / Fish / Immunology / Evolution
研究実績の概要

The major experimental achievement this year is that we found that the rainbow trout cytokines IL-2, IL-15, and IL-15L have functional effects on trout lymphocytes. We are currently writing those results into an article. The function of IL-15L we like to keep secret here, because it concerns a newly discovered cytokine. Important for vaccine adjuvants purposes is that we found that trout IL-2 and IL-15 can stimulate cytotoxic cells if presented together with IL-15Ra, which agrees with expectations based on studies in mammals. Although for bovine IL-2, IL-15 and IL-21 we could find the expected functions, we have not been able so far to find a function for bovine IL-15L.

現在までの達成度 (区分)
現在までの達成度 (区分)

3: やや遅れている

理由

We are very glad that all the trout and bovine cytokines that we expressed recombinantly, with the exception of bovine IL-15L, are functional and able to affect lymphocytes. The writing of those data into a publication is in good progress. However, the expression of soluble proteins in other systems than mammalian cells takes more time than we expected.

今後の研究の推進方策

The accumulating trout data are most promising and important to us, so we will continue analyzing the trout IL-2, IL-15 and IL-15L molecules. So far we have worked with the recombinant trout proteins from the supernatants of transfected mammalian cells, which is good in the sense that these proteins should be properly folded. But we need higher amounts for efficient analysis of various functions, including vaccine adjuvant properties. Therefore this year will be especially important for the recombinant production of the trout cytokines in other expression systems such as E.coli and insect cells. This will include free cytokines, as well as cytokines genetically fused to IL-15Ra. The assays in which the recombinant cytokines will be tested will be biased towards finding the best conditions for stimulation of cytotoxic T cells. We hope to achieve similar results as those that we obtained with the trout cytokines expressed by transfected mammalian cells.

  • 研究成果

    (6件)

すべて 2015 2014

すべて 雑誌論文 (3件) (うち国際共著 1件、 査読あり 3件、 オープンアクセス 2件、 謝辞記載あり 1件) 学会発表 (3件)

  • [雑誌論文] A comprehensive analysis of teleost MHC class I sequences.2015

    • 著者名/発表者名
      Grimholt U, Tsukamoto K, Azuma T, Leong J, Koop BF, Dijkstra JM.
    • 雑誌名

      BMC Evol Biol.

      巻: 15 ページ: 32

    • DOI

      10.1186/s12862-015-0309-1

    • 査読あり / オープンアクセス / 国際共著 / 謝辞記載あり
  • [雑誌論文] Non-human lnc-DC orthologs encode Wdnm1-like protein.2014

    • 著者名/発表者名
      Dijkstra JM, Ballingall KT
    • 雑誌名

      F1000Research

      巻: 3 ページ: 160

    • DOI

      10.12688/f1000research.4711.2

    • 査読あり / オープンアクセス
  • [雑誌論文] TH2 and Treg candidate genes in elephant shark.2014

    • 著者名/発表者名
      Dijkstra JM
    • 雑誌名

      Nature

      巻: 511 ページ: E7-9

    • DOI

      10.1038/nature13446

    • 査読あり
  • [学会発表] Review on the (expected) prowess of the cytokine family comprising interleukins 2, -15 and -15-like to initiate an anti-cancer immune status.2015

    • 著者名/発表者名
      Johannes M Dijkstra, Takuya Yamaguchi, Uwe Fischer, Keiichiro Hashimoto
    • 学会等名
      Tumor Immunology: Multidisciplinary Science Driving Combination Therapy (J7), organized by Keystones Symposia,
    • 発表場所
      Banff, Canada Fairmont Banff Springs
    • 年月日
      2015-01-08 – 2015-01-14
  • [学会発表] An ancient cytokine family including IL-2, IL-15 and IL-15-like (IL-15L).2014

    • 著者名/発表者名
      Takuya Yamaguchi, Uwe Fischer, Keiichiro Hashimoto, and Johannes M. Dijkstra
    • 学会等名
      The 43rd annual meeting of the Japanese Society for Immunology.
    • 発表場所
      Kyoto, Japan Kyoto International Conference Center
    • 年月日
      2014-12-10 – 2014-12-12
  • [学会発表] Evolution of the interleukin 2, 15 en 15-like family.2014

    • 著者名/発表者名
      Takuya Yamaguchi, and Johannes M. Dijkstra.
    • 学会等名
      26th Annual Meeting of the Japanese Association for Developmental and Comparative Immunology (JADCI).
    • 発表場所
      Sendai, Japan TOHOKU UNIVERSITY
    • 年月日
      2014-07-09 – 2014-07-11

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公開日: 2016-05-27  

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