| 研究課題/領域番号 |
25560231
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| 研究機関 | 独立行政法人理化学研究所 |
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研究代表者 |
MALAY Ali 独立行政法人理化学研究所, Heddle国際主幹研究ユニット, 研究員 (40467006)
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| 研究期間 (年度) |
2013-04-01 – 2016-03-31
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| キーワード | protein / gold nanoparticle / synthetic biology / protein engineering / bionanotechnology |
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| 研究実績の概要 |
Last year we made significant progress in understand the structure and dynamic mechanism of artificial capsid formation of TRAP-cage. In terms of mechanism, it was revealed that gold nanoparticles payed a role not as catalysts as originally imagined, but rather gold atoms are incorporated specifically as thiol bridges in a process related to "thiol etching" of gold nanoparticles. In terms of structure, we now have a nearly complete model, showing that TRAP-cage is composed of 24 11mer rings (total of 264 subunits) and 120 gold atoms, in a unique symmetry never before seen in protein cage structures. Finally, analysis of material properties of TRAP-cage have shown it to be an exceptionally stable structure, able to resist boiling, yet can readily disassemble in response to changes in redox conditions. Some findings, suing high-speed AFM, have been published in Nano Letters.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
2: おおむね順調に進展している
理由
Significant progress was made in terms of elucidating the structure, mechanism, and nanomaterial properties of TRAP-cage.
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| 今後の研究の推進方策 |
We would like to develop applications for TRAP-cage, such as for use a redox-responsive delivery systems for nanoscale cargo molecules. Other highly promising applications are also considered.
Several questions still remain, especially relating to the nature of the Au-S interactions that lead to the formation of TRAP-cage, for which we would need to collaborate with specialists in the field of gold chemistry.
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| 次年度使用額が生じた理由 |
Due to the difficult nature of the project, publication of results have been delayed; therefore, some funds have been left over.
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| 次年度使用額の使用計画 |
Most of the funds will be used to defray publication costs. Additionally, additional experimental reagents and equipment will be purchased, and some of the funds will be used to pay conference fees, etc.
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