| 研究成果の概要 |
On development of intra-cytoplasmic sperm injection (ICSI) in the silkworm, it is important to detect the cell cycle restart of unfertilized egg and discover triggers of the restart. We could detect the cell cycle restart of unfertilized egg examining phosphorylation or dephosphorylation of Mitogen-activated Protein Kinase (MAPK) as the evaluation index. And our results indicates that heat shock treatment make this restart.
In mammals it is known that freeze-dried sperms have fertility in ICSI. To investigate whether it is effective in insects as mammals or not, we examined using the sawfly, Athalia rosae in which ICSI is available. We tried the injection of freeze-dried sperms into unfertilized egg in about 1000 scale growing 200 embryos in which we found only one embryo which express marker gene derived from sperm, indicating the effectivity of ICSI using freeze-dried sperms in insect.
|
