研究課題
We have achieved the following results:i)Initiating strain-specific transmission-blocking immunity in Plasmodium yoelii. We have performed numerous experiments using natural immunity to block transmission in P. yoelii. We have shown that there is a sharp decrease in the infectivity of gametocytes throughout the course of an infection in mice, and, using IFN-Gamma and MyD88 KO mice, that this is independent of the innate immune response. We have used sera transfer to demonstrate that sera from mice with long-term infections supresses the ability of early-infection stage gametocytes to transmit, and that this suppression has a strain-specific component.ii)We are currently investigating the feasibility of performing LGS experiments with P. falciparum. Using the strains 3D7 and HB3, we have cultured pure gametocytes, and purified crude protein, to produce immune sera in rabbits. We have acquired the uncloned recombinant progeny of a cross between these two strains.iii)We have developed bioinformatics and statistic techniques capable of identifying statistically significant deviations in the relative frequency of SNP markers measured in whole genome resequencing analyses of recombinant progeny populations of malaria parasites following selection.iv)We have shown that there is no link between virulence and transmission success in malaria parasites.
すべて 2016 2015
すべて 雑誌論文 (5件) (うち国際共著 5件、 査読あり 5件、 オープンアクセス 5件) 学会発表 (1件)
Antimicrobial Agents and Chemotherapy
巻: 印刷中 ページ: 印刷中
10.1128/AAC.02370-15
Parasites & Vectors
巻: 8 ページ: 376-376
10.1186/s13071-015-0995-y
巻: 8 ページ: 318-318
10.1186/s13071-015-0927-x
Acta Tropica
巻: 148 ページ: 1-7
10.1016/j.actatropica.2015.04.007
Trends in Parasitology
巻: 31 ページ: 232-238
10.1016/j.pt.2015.03.003.
