| 研究課題/領域番号 |
26288023
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| 研究機関 | 名古屋大学 |
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研究代表者 |
CRUDDEN Cathleen 名古屋大学, トランスフォーマティブ生命分子研究所, 客員教授 (10721029)
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| 研究期間 (年度) |
2014-04-01 – 2017-03-31
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| キーワード | nanoparticle / nanocluster / N-heterocyclic carbene / gold / bioimaging / bioconjugation |
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| 研究実績の概要 |
We were able to prepare several types of Au nanoparticles using different stabilizers for the external surface. These nanoparticles were then exposed to two different conditions for generation of surface carbenes and three basic carbene structures were employed.
Gold nanoclusters stabilized by thiol ligands, and less often by phosphines have been reported, but the use of NHCs to stabilize these nanomaterials has never been described. In preliminary work, we have prepared phosphine-stabilized nanoclusters and thiol-stabilized clusters. In order to assess the effectiveness of different reaction conditions, we have only just recently developed a mass spectroscopic method, and we anticipate this work will move much more quickly henceforth.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
3: やや遅れている
理由
Progress-to-date has been good but has not followed exactly with the projected plan, instead we have completed some milestones from year one along with some milestones from year two. We were delayed several months by the discovery that the literature method for the preparation of the carbonate salts was flawed. Canadian labs since this affected related work going on in Canada, and with this in place, we are now set to continue with this functionalization strategy and also that employing the free carbene, which, according to work described above, appears to work very well.
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| 今後の研究の推進方策 |
In year two, we plan to finish work on the synthesis of NHC-stabilized nanoparticles and nanoclusters using simple NHCs. At the same time, we will complete work started to probe thiol incorporation under physiological conditions. Nanoparticle and nanocluster stability under physiological conditions will also be probed and once these variables have been optimized, derivatized NHCs will be introduced to the nanoparticle so that we can carry out bioconjugation studies. Simultaneously, we will carry out bioconjugation studies on flat gold surfaces that are easier to analyze in order to translate this work to the nanoparticle realm more smoothly, and will then carry out this work on nanoparticles.
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| 次年度使用額が生じた理由 |
We were delayed several months by the discovery that the literature method for the preparation of the carbonate salts was flawed. Thus we decided to roll over the fund next year.
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| 次年度使用額の使用計画 |
We are planing to use for employment of postdoc to promote this project.
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