| 研究課題/領域番号 |
26290011
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| 研究機関 | 独立行政法人理化学研究所 |
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研究代表者 |
ベヌッチ アンドレア 独立行政法人理化学研究所, 脳科学総合研究センター, チームリーダー (50722352)
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| 研究期間 (年度) |
2014-04-01 – 2017-03-31
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| キーワード | mouse / visual cortex / imaging / electrophysiology / behavior / decision making / optogenetics |
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| 研究実績の概要 |
The Laboratory has operated for most of the fiscal year only with the aid of technical staff. Starting on January 1st one postdoctoral fellow has joined the lab and from the beginning of FY2015 four more fellows have been joining the lab.
Progress on animal behavior: the 1st generation of automated behavioral setups has been optimized and we are progressing to the 2nd generation. The 1st generation has confirmed the feasibility of fully-automated training in several animals, both for visual detection and discrimination tasks. The 2nd generation has just started being set-up.
Progress on Imaging and electrophysiology: we have finished setting up the two-photon imaging system for in-vivo calcium imaging and obtained several high-quality test results in a number of transgenic animals. We have also fine-tuned a wide-field imaging system for fluorescent recordings of multiple visual areas. It has produced very high-quality test results. The electrophysiology setup has been successfully setup and it is currently in intense testing phase.
Progress on Genetics, viral approaches: we have tested and develop several viral constructs that we consider suitable for our experiments. 80% of the constructs of interest have been fully tested and will be either kept or tossed in future experiments. 20% are still in an intense benchmarking phase.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
2: おおむね順調に進展している
理由
The progress has been very smooth and according to the planned timeline.
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| 今後の研究の推進方策 |
Plan on animal behavior: finish the 2nd generation of behavioral training setups, train mice in even more complex behavioral tasks, and collect more data to validate projects` hypotheses.
Plan on Imaging and electrophysiology: finishing the testing phase on in-vivo fluorescent Calcium imaging, focus on hypothesis testing by recording from animals trained in behavioral tasks. Extensively test new triple-transgenic animals(Madisen,..., Benucci, et al., Neuron 2015).Finishing testing phase for electrophysiology.
Plan on Optogenetics: finish testing a number of constructs for activity-dependent expression of optogenetic constructs. Apply the chosen tools to animals trained in behavioral tasks.
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| 次年度使用額が生じた理由 |
The optimization of the behavioral setups for training mice has required a longer than expected optimization process, both for the software and hardware. Hence we did not purchase as many setups (and related components) as originally planned.
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| 次年度使用額の使用計画 |
The optimization of the mouse behavioral setups is now progressing smoothly, hence we intend to scale-up and purchase more of them. Moreover, new postdoctoral fellows have joined the lab and the personnel costs will grow accordingly. In particular, I am planning to use available funds to allow them to travel domestically to meetings and schools.
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