| 研究課題/領域番号 |
26505010
|
|---|
| 研究機関 | 横浜市立大学 |
|---|
研究代表者 |
ROBERT Kanaly 横浜市立大学, 生命ナノシステム科学研究科, 准教授 (00448656)
|
|---|
| 研究期間 (年度) |
2014-04-01 – 2017-03-31
|
| キーワード | DNA adductomics |
|---|
| 研究実績の概要 |
Model organism was decided and it was determined that it produced PAH-type catechols during biotransformation of PAHs. Genes involved in catabolism of PAHs by this organism were determined. RT-PCR methods were established by investigating expression of these genes. Assessment of DNA damage by LC/ESI-MS/MS DNA adductomics was conducted using the model organism and model toxicant hydrogen peroxide.
|
| 現在までの達成度 (区分) |
現在までの達成度 (区分)
2: おおむね順調に進展している
理由
Determined model organism. Determined that this organism biotransforms PAHs through a PAH-catechol type pathway by analyzing biotransformation products during exposure to PAHs. Established methods for conducting RT-PCR analyses by investigating genes involved in PAH catabolism.
|
| 今後の研究の推進方策 |
Continue DNA damage analyses using hydrogen peroxide model toxicant. Begin investigation of DNA damage by using PAH o-quinones. Continue RT-PCR analyses. Develop methods for detecting ROS by fluorescence in model organism. Continue to develop software for processing DNA adductome data.
|
| 次年度使用額が生じた理由 |
Budget from 2014-15 was carried over to 2015-16 because it was sufficient for the experiments conducted in 2014-15.
|
| 次年度使用額の使用計画 |
Continuation of experiments. Publication fees. Budget for travel to present results.
|
