環境保全微生物に生じたDNAダメージと修復のアダクトミクスに関する研究

2016 年度 実施状況報告書

• 研究課題/領域番号: 26505010
• 研究機関: 横浜市立大学
• 研究代表者: Robert Kanaly 横浜市立大学, 生命ナノシステム科学研究科(八景キャンパス), 教授 (00448656)
• 研究期間 (年度): 2014-04-01 – 2018-03-31
• キーワード: DNA adductomics

研究実績の概要

Exposure assays using hydrogen peroxide and 1,2naphthoquinone were conducted. Some putative DNA adducts detected in bacterial cells were identified as DNA adducts by different techniques. Relative levels of known and unknown DNA adducts were compared. Methods were developed for conducting ROS assays. Initial methods for conducting RNA transcriptome analyses were explored.

現在までの達成度 (区分)

2: おおむね順調に進展している

理由

Some putative DNA adducts were identified but require further confirmation. ROS assay assays showed that ROS levels were related to exposure scenarios. Using bacteria, DNA adductomics system was established and has allowed for wider application using human cell lines.

今後の研究の推進方策

Complete RT-PCR analyses, confirm unknown adducts, and conduct RNA transcriptomics.

次年度使用額が生じた理由

Budget was carried over for the purpose to complete transcriptome analyses.

次年度使用額の使用計画

Plan to conduct transcriptome analyses.

研究成果

• [雑誌論文] Internalization of a novel, huge lectin from Ibacus novemdentatus (slipper lobster) induces apoptosis of mammalian cancer cells (2017)
  • 著者名/発表者名: Fujii, Y., T. Fujiwara, Y. Koide, I. Hasan, S. Sugawara, S. Rajia, S. M. A. Kawsar, D. Yamamoto, D. Araki, R. A. Kanaly, Y. Ogawa, H. Fujita, and Y. Ozeki
  • 雑誌名: Glyconjugate Journal 巻: 34 ページ: 85-94
  • DOI: 10.1007/s10719-016-9731-x
  • 査読あり / 国際共著
• [雑誌論文] RAMA casein zymography: time-saving and highly sensitive casein zymography for MMP7 and trypsin (2016)
  • 著者名/発表者名: Yasumitsu, H., Y. Ozeki, and R. A. Kanaly
  • 雑誌名: Electrophoresis 巻: 37 ページ: 2959-2962
  • DOI: 10.1002/elps.201600346
  • 査読あり / 国際共著
• [雑誌論文] Analysis of the fine-tuning of cyanobacterial circadian phase by monochromatic light and long-day conditions (2016)
  • 著者名/発表者名: Kobayashi, T., Y. Obana, N. Kuboi, Y. Kitayama, S. Hayashi, M. Oka, N. Wada, K. Arita, T. Shimizu, M. Sato, R.A. Kanaly and S. Kutsuna
  • 雑誌名: Plant and Cell Physiology 巻: 57 ページ: 105-114
  • DOI: 10.1093/pcp/pcv177
  • 国際共著
• [学会発表] Biodegradation of high concentrations of model fuel hydrocarbon NAPLs by a bacterium isolated from soil (2016)
  • 著者名/発表者名: Takeshita, T., and R. A. Kanaly
  • 学会等名: The 31st Annual Meeting of the Japanese Society of Microbial Ecology
  • 発表場所: Yokosuka, Kanagawa, Japan
  • 年月日: 2016-10-22 – 2016-10-25
  • 国際学会
• [学会発表] Bacterial biodegradation of sulfur-containing aromatic pollutants and the production of disulfides measured by LC-ESI-MS2 (2016)
  • 著者名/発表者名: Ozawa, K., Nemoto, Y., Kikuchi, M., and R. A. Kanaly
  • 学会等名: The 31st Annual Meeting of the Japanese Society of Microbial Ecology
  • 発表場所: Yokosuka, Kanagawa, Japan
  • 年月日: 2016-10-22 – 2016-10-25
  • 国際学会
• [学会発表] Biodegradation of benzo[a]pyrene by Sphingobium barthaii KK22 and effects on co-occurring PAH biodegradation (2016)
  • 著者名/発表者名: Izawa, M., and R.A. Kanaly
  • 学会等名: The 31st Annual Meeting of the Japanese Society of Microbial Ecology
  • 発表場所: Yokosuka, Kanagawa, Japan
  • 年月日: 2016-10-22 – 2016-10-25
  • 国際学会
• [学会発表] Chemical and genetic analyses of polycyclic aromatic hydrocarbon biodegradation by Sphingobium barthaii KK22 (2016)
  • 著者名/発表者名: Maeda, A.H., S. Nishi, Y. Hatada, Y. Ohta, Y. Ozeki, M. Kunihiro and R.A. Kanaly
  • 学会等名: The 31st Annual Meeting of the Japanese Society of Microbial Ecology
  • 発表場所: Yokosuka, Kanagawa, Japan
  • 年月日: 2016-10-22 – 2016-10-25
  • 国際学会
• [学会発表] Biotransformation of PAHs by Sphingobium barthaii and investigation of DNA damage by LC/ESI-MS/MS (2016)
  • 著者名/発表者名: Fukuoka, K., and R.A. Kanaly
  • 学会等名: ASM Microbe 2016
  • 発表場所: Boston, MA, USA
  • 年月日: 2016-06-16 – 2016-06-20
  • 国際学会