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2017 年度 実績報告書

環境保全微生物に生じたDNAダメージと修復のアダクトミクスに関する研究

研究課題

研究課題/領域番号 26505010
研究機関横浜市立大学

研究代表者

Robert Kanaly  横浜市立大学, 生命ナノシステム科学研究科(八景キャンパス), 教授 (00448656)

研究期間 (年度) 2014-04-01 – 2018-03-31
キーワードDNA adductomics
研究実績の概要

Exposure and DNA adductomics analyses of extracted 2'-deoxynucleosides from model organism were continued by LC/ESI-MS/MS. ROS assays were continued. RNA transcriptomics overall experimental plan and methods for RNA extraction were developed and conducted. RNA-seq data were obtained from experiments where model organism was either exposed or unexposed to 1,2-naphthoquinone. Developed post-analysis methods for comparative analyses of RNA-seq data. Conducted analyses. Differentially expressed genes were detected under the condition of FDR value less than or equal to 0.00001 with values showing at least a 2-fold difference between conditions. Differentially expressed genes were tabulated and annotated for identification purposes. Overall, data interpretation, organization and synthesis are ongoing. Preparation of manuscript(s) for submission to international peer-review journals is in progress.

  • 研究成果

    (3件)

すべて 2018 2017

すべて 学会発表 (3件)

  • [学会発表] Investigation of intracellular RNA damage to improve chemical safety assessment methods2018

    • 著者名/発表者名
      Takeshita, T., and R.A. Kanaly
    • 学会等名
      The Seventh Intercollegiate Conference on Science in Japan, Japan Ministry of Education, Culture, Sports, Science and Technology Mar 2018
  • [学会発表] Comprehensive analysis of DNA adducts in human liver cells after exposure to safrole2017

    • 著者名/発表者名
      Takeshita, T., F. Tao, N. Kojima and R.A. Kanaly
    • 学会等名
      The 23st General Meeting of the Japanese Society of Food Chemistry
  • [学会発表] Investigation of reactive metabolites in human liver cell line by use of the DNA adductome method2017

    • 著者名/発表者名
      Takeshita, T., F. Tao, N. Kojima and R.A. Kanaly
    • 学会等名
      Consortium of Biological Sciences 2017 (ConBio2017)

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公開日: 2018-12-17  

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