Enhanced Cell Evaluation by Phase Decomposition

2014 年度 実施状況報告書

• 研究課題/領域番号: 26820086
• 研究機関: 大阪大学
• 研究代表者: 蔡 佳宏 大阪大学, 工学(系)研究科(研究院), 研究員 (20647490)
• 研究期間 (年度): 2014-04-01 – 2016-03-31
• キーワード: cell deformability / red blood cell / microfluidics / lab on a chip

研究実績の概要

A total of five papers, including 2 SCI-indexed journal papers and 3 conference papers, are published in the last year of this research project.

The experimental platform for the proposed cell evaluation has been successfully developed, and is capable of manipulating a cell moving in a simple harmonic motion up to 130 Hz (Monzawa, Kaneko, Tsai et al. Biomicrofluidics 9(1)p014114, 2015). Microfluidic channel designs that can align cells have been developed for enhancing the evaluation system (Tsai et al. RSC Advances, 4(85)p45050-45058,2014 and IEEE-URAI2014, p522-524). The difference of mechanism between cell flow under a positive-pressure and negative-pressure driven flow is investigated (MicroTAS2014, p793-795). An on-chip pressure sensor for assisting the evaluation of cell deformability is developed (IEEE MEMS2015, p722-725).

現在までの達成度 (区分)

2: おおむね順調に進展している

理由

The plan of the first year in the proposal is to build up the experimental system and have preliminary tests on cell deformability. The current progress smoothly catches up with the plan. The hardware of the system has been set up, and the image-processing algorithm for cell tracking has been developed. The deformability of the red blood cells from a patient with Multiple Myeloma is found very different from the ones with healthy subjects. The results have been published in International Journal with impact factor of 3.7.

今後の研究の推進方策

There are two aims for this year. The first aim is to further investigate the relation between cell deformability and human diseases. It has been scheduled for the experiments on the red blood cells from the patients with cardiovascular circulation problems. The second aim is to improving the proposed evaluation system, so that it can be more accurate on deformability evaluation and more convenient for everyone to use.

研究成果

• [雑誌論文] On-Chip Actuation Transmitter for Enhancing the Dynamic Response of Cell Manipulation using a Macro-scale Pump (2015)
  • 著者名/発表者名: T. Monzawa, M. Kaneko, C. Tsai, S. Sakuma and F. Arai
  • 雑誌名: Biomicrofluidics 巻: 9 ページ: 014114
  • DOI: 10.1063/1.4907757
  • 査読あり / 謝辞記載あり
• [雑誌論文] Geometrical Alignment for Improving Cell Evaluation in a Microchannel with Application on Multiple Myeloma Red Blood Cells (2014)
  • 著者名/発表者名: C. Tsai, S. Sakuma, F. Arai, T. Taniguchi, T. Ohtani, Y. Sakata and M. Kaneko
  • 雑誌名: RSC Advances 巻: 4 ページ: 45050-45058
  • DOI: 10.1039/C4RA08276A
  • 査読あり / 謝辞記載あり
• [学会発表] On-Chip Pressure Sensing by Visualizing PDMS Deformation using Microbeads (2015)
  • 著者名/発表者名: Chia-Hung Tsai
  • 学会等名: The 28th IEEE Conference on Micro Electro Mechanical Systems (MEMS 2015)
  • 発表場所: Estoril Centro De Congressos, Estoril, Portugal
  • 年月日: 2015-01-18 – 2015-01-22
• [学会発表] Improving the Evaluation of Cell Deformability by Different Channel Width in a Microfluidic Device (2014)
  • 著者名/発表者名: Chia-Hung Tsai
  • 学会等名: The 11th Int. Conf. on Ubiquitous Robots and Ambient Intelligence (URAI14)
  • 発表場所: Double Tree Hotel, Kuala Lumpur, Malaysia
  • 年月日: 2014-11-12 – 2014-11-15
• [学会発表] What is the Difference of Cell Deformation between PUSH and PULL? (2014)
  • 著者名/発表者名: Shinya Sakuma
  • 学会等名: Proceeding of the 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences (microTAS14)
  • 発表場所: Henry B. Gonzalez Convention Center, San Antonio, USA
  • 年月日: 2014-10-26 – 2014-10-30