| 研究実績の概要 |
1. Analysis shape and motion of cells fast-flowing in microchannels
1) We performed several experiments using sea urchin egg cells fast-flowing in a straight microchannel. To compare the difference between normal and Bouin's fixed sea urchin egg cells, all experiments were performed using those two kinds of sea urchin egg cells. In the first experiment, sea urchin egg cells flowing at different speeds were observed to quantify how their shapes are deformed in fast microchannel flows. In the second experiment, sea urchin egg cells were observed to quantify their aged deterioration after spawning by inspecting their deformed shapes in a fast microchannel flow. 2) To confirm that our system can detect transient variations in fast-flowing cells in irregular microchannels, we performed an experiment using normal sea urchin egg cells fast-flowing in an L-type microchannel. We verified that temporary changes in the cells' shape and motion in the corner of the L-type microchannel can be detected using our system.
2. Perform fast recognition of fertilized cells fast-flowing in microchannels
In this experiment, we performed fast cell recognition of fertilized sea urchin egg cells fast-flowing in straight microchannels, on an hourly basis by using a hardware extracted bounding box of cells. The flow speed at 500 ul/min for all experiments and real-time image-based cell recognition was working at 2000 fps with a resolution of 512×256. This experiment showed that our system can be used for high-frame-rate, image-based (ROI) shape recognition and analysis.
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