| 研究実績の概要 |
Gingival crevice (GC) increases with age allowing periodontopathic bacteria and its products to enter. We hypothesize that by mimicking this event we can utilize the GC as a potential vaccination route. We performed the following: (1) elucidated the difference between oral-administration and oral-supplementation in both young and old rats; (2) determined the optimal component concentration for xanthan gel-encapsulation; (3) simulated xanthan gel-encapsulation in order to verify that target epitopes were not blocked; (4) compared the antibody titer among gingival-vaccinated rats (old and young); and (5) evaluated the antibody titer produced via the gingival route as compared to other vaccination routes (intradermal, oral, sublingual). Throughout the study, we were able to establish the following conditions: (1) higher target components enter old rats via oral-supplementation; (2) 100 microgram per mL is the optimal component concentration for xanthan gel-encapsulation; and (3) xanthan gel-encapsulation leaves antibody epitopes exposed. More importantly, we observed that gingival-vaccinated old rats have higher antibody titer as compared to young rats and, likewise, we found that antibody titer elicited via gingival vaccination is comparable to other mucosal vaccination routes.
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