| 研究課題/領域番号 |
26890003
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| 研究機関 | 筑波大学 |
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研究代表者 |
VOGT Kaspar 筑波大学, 国際統合睡眠医科学研究機構, 准教授 (80740034)
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| 研究期間 (年度) |
2014-08-29 – 2016-03-31
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| キーワード | Sleep / DREADD / Slow Wave / Homeostasis |
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| 研究実績の概要 |
We have successfully established a novel recording system derived from highly integrated microcircuits by Intan Inc. These chips perform signal amplification and digitization, resulting in a direct digital data stream, which is far more resistant to noise interference than analog signals. It also allows to carry many signals over few wires through multiplexing. We have performed long-term EEG recordings with this system and have performed detailed EEG analysis on the resulting data. We have also successfully transfected mice with AAV for reporter proteins and for activating and inhibiting DREADDs. We are currently analyzing the results of EEG recordings in DREADD transfected animals under control conditions and under CNO injections.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
2: おおむね順調に進展している
理由
The technical aspects of the project are progressing verz well. We do not have extensive data on DREADD transfected mice, but we are expecting that we can perform these experiments shortly.
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| 今後の研究の推進方策 |
We are working on local field potential electrode implantations to increase the spatial resolution of our recordings. We will continue to work with AAV DREADD injections in different Cre mouse lines to study different neuronal populations and their contribution to slow wave activity.
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