| 研究実績の概要 |
To monitor three types of responses at VIN3 to cold (acute induction, long term quantitative induction, priming response) an experimental system was standardised. Detailed chromatin profiling was completed for three histone modifications in this system and additional ones will be completed based on the same standard. Based on six time points of a standard cold priming assay, i identified two types of modifications that appear to condition the priming response, as their levels do not drop during the recovery period. Development of tools to dissect the DNA level regulation of the VIN3 chromatin locus was initiated. Using CRISP CAS9 technology, eight non-coding regions of the VIN3 locus are now targeted for genome editing.
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| 今後の研究の推進方策 |
Many plants flower in spring, after experiencing prolonged exposure to the cold of winter because FLC, a potent inhibitor of flowering, is attenuated during cold and remains in a state of repression, stable mitoticaly after cold. VIN3, encoding a PHD-domain protein, is quickly induced by cold; it physically associates with a chromatin modifying complex PRC2 that controls repression of FLC. This is the main known biochemical function of VIN3. Interestingly, VIN3 increases quantitatively in the cold for as long as the cold treatment lasts. Moreover, VIN3 expression is induced faster during a second exposure to cold after a warm recovery stage, i.e. VIN3 is primed by cold. How quantitative and priming responses intersect has not been described.
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