| 研究実績の概要 |
In this research, we demonstrated a visualization of photolysis reaction by a synthetic metal complex using TR-SFX method. We fixed a photoactive CO releasing Mn(CO)3 reaction center into porous Lysozyme microcrystals. X-ray crystal structure revealed an octahedral Mn reaction center with 3CO and two water molecules. Three CO ligands oriented in a facial manner. By controlling the time and light dose, we succeeded in determining the CO release process and structure of a bicarbonyl intermediate species in real-time. In addition, we performed quantum chemical calculations which was in good agreement with the experimental results. The demonstration has a great scope to explore the mechanism of difficult organic, inorganic, or synthetic metalloprotein reactions.
|
