| 研究領域 | 新興硫黄生物学が拓く生命原理変革 |
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| 研究課題/領域番号 |
22H05560
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| 研究機関 | 東京工業大学 |
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研究代表者 |
CATTI LORENZO 東京工業大学, 科学技術創成研究院, 助教 (50873478)
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| 研究期間 (年度) |
2022-06-16 – 2024-03-31
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| キーワード | Supersulfide / MS analysis / Redox processes |
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| 研究実績の概要 |
The mass detection of S8 was achieved in collaboration with the Akaike group. The supramolecular coordination capsule was found to have good cell penetration abilities. Inside the cell, the capsule encapsulated S8 and the thus stabilized S8 was detected via MS analysis. This strategy was also utilized for the quantification of S8 within cells. The results furthermore indicate that S8 serves an important role within cells for redox processes. This discovery might lead to the development of supersulfide medicines.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
1: 当初の計画以上に進展している
理由
The supramolecular capsule displayed unexpectedly well cell-membrane permeability and bound S8 within the complex cell environment. The MS analysis indicated a significant concentration of S8 within cells. The preparation of new coordination capsules for binding of other (smaller or larger than S8) supersulfide species is also proceeding smoothly.
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| 今後の研究の推進方策 |
Besides MS analysis, we are planning the fluorescence analysis of S8 using our supramolecular capsule tool. For this we will utilize a fluorophore replacement essay. Both fluorescence Turn-ON and Turn-OFF sensing will be targeted. We also plan the synthesis of S8-binding supramolecular capsules with higher water-solubility. Internally functionalized capsules are also planned.
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