| 研究領域 | マイクロエンドフェノタイプによる精神病態学の創出 |
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| 研究課題/領域番号 |
25116529
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| 研究種目 |
新学術領域研究(研究領域提案型)
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| 研究機関 | 独立行政法人理化学研究所 |
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研究代表者 |
MCHUGH Thomas 独立行政法人理化学研究所, 脳科学総合研究センター, チームリーダー (50553731)
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| 研究期間 (年度) |
2013-04-01 – 2015-03-31
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| キーワード | 海馬 hippocampus / トランスジェニックマウス / 統合失調症 schizophrenia / アデノ随伴ウィルス |
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| 研究概要 |
During the FY2013 we have achieved several major milestones:
(1) We have achieved highly reproducible strong expression of the ClCtx peptide specifically in CA2 via viral infection of the CACNG5-cre line. The primary improvement we made to earlier attempts was to adopt the AVV-DJ/8 serotype as our primary viral vector. We have perfected in-house high-titer production of this virus and this has allowed highly reproducible experiments.
(2) We have conducted in vivo physiological recordings in the hippocampi of mice infected with the ClCtx virus; analyses of these data is ongoing.
(3) We have started behavioral experiment on ClCtx expressing mice with a focus on the impact of disinhibition on contextual exploration and social behavior.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
3: やや遅れている
理由
The in vitro portion of the project, namely testing the effectiveness of the ClCtx in hippocampal slice, has progressed slower than planned. The primary reason is the access to the appropriate equipment, namely an invitro recording setup with whole cell patch capabilities, has been difficult to obtain, however through internal collaboration we hope to resolve this issue shortly.
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| 今後の研究の推進方策 |
This ongoing work was presented at the マイクロ精神病態 meeting in Nagoya in September, 2013. This meeting provided an excellent opportunity to share our progress and receive valuable and insightful comments from colleagues.
In the coming fiscal year the primary goal of these experiments will be to complete the behavioral and in vivo physiological characterization of the CA2-Chlorotoxin expressing mouse. Recent publications have indicated that silencing CA2 output via tentanus toxin expression leads to social memory deficits. We anticipate that our manipulation, which should increase CA2 output, may have a similar phenotype. The aim of our behavioral experiments are to assess social interaction, social memory and social habituation. The in vivo recordings will be focused on confirming the physiological impact of the toxin expression.
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