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Stress protein and fiber type

Research Project

Project/Area Number 04454587
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 体育学
Research InstitutionUniversity of Tokyo

Principal Investigator

ATOMI Yoriko  Univ.of Tokyo College of Arts & Sciences Associate Prof., 教養学部, 助教授 (90125972)

Co-Investigator(Kenkyū-buntansha) HATTA Hidro  Univ.of Tokyo College of Arts & Sciences Assistant, 教養学部, 助手 (60208535)
ISII Naokata  Univ.of Tokyo College of Arts & Sciences Associate Prof., 教養学部, 助教授 (20151326)
Project Period (FY) 1992 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥6,300,000 (Direct Cost: ¥6,300,000)
Fiscal Year 1993: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1992: ¥4,900,000 (Direct Cost: ¥4,900,000)
KeywordsalphaB-crystallin / Stress protein / fiber type / tyroid holmone / 筋繊維組成 / ラット後肢懸垂法 / ストレッチ
Research Abstract

The sequence of alphaB-crystallin has striking similarity to the suquence of small heat shock proteins (HSPs#) and increasing evidence indicates that it has also functional similarities with the small HSPs. The expression of alphaB-crystallin is induced upon stress. Similarly, amorphous alphaB-acrystallin aggregates are formed after ischemia. alphaB-crystallin can function as a molecular chaperone, by suppressing heat induced aggregation of other proteins, and by assisting refolding of denatured protein. These observations from in vitro experiments suggest that alphaB-crystallin has to be considered a member of the small HSPs. It seems reasonable to assume that the constitutive expression is required in soleus muscle because this muscle is continuously subjected to a more stressful environment, such as high temperature, oxidant injury, and higher rate of protein turnover, than other muscles and requires stress related functions provided by alphaB-crystallin. This drives us to the question whether the expression of alphaB-crystallin in stressful muscle is directly regulated with its high rate recruitment, which causes stressful environment, or indirectly regulated in a manner of expression as a fiber type specific protein. The relationship between the expression of alphaB-crystallin as stress protein and fiber-type were examined in three experiments of 1) those distributions in control rats, 2) relative changes due to muscle atropphy by hindlimb suspension, and 3) relative changes due to the treatment of thiroid hormone and PTU.For this purpose we used immuno-histochemical method to investigate these relationship using mouse monoclonal anti-myosin antibodies and rabbit anti-alphaB-crystallin polyclonal antibodies. In soleus muscles, type I fibers reacted much stronger with anti-alphaB-crystallin Ig, than type IIA fibers. In EDL muscles, type I fibers showed strong reactivity for anti-alphaB-crystallin Ig than type II fibers. In addition to the differences between ty

Report

(3 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • Research Products

    (15 results)

All Other

All Publications (15 results)

  • [Publications] Yoriko,Atomi: "Decreased αB-crystallin in soleus muscle atrophy and role of αB-crystallin in muscle" In integration of Medical and Sport Sciences(Edited by Sato et al). 171-192 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] T.Masuda and Y.Atomi: "Fiber-type dependent distribution of αB-crystallin in rat skeletal muscle" J.Appl.Physiol.(to be submitted).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] H.Hatta,R.Soma,and Y.Atomi: "Effect of DCA administration or endrance training on lactate metabolism in mice during and after exercise" Ann.Physiol.9. 275-278 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 新井秀明,山下雅道,跡見順子: "伸張刺激に対する培養筋細胞の形態とアクチンフィラメントの応答-培養細胞伸張装置の開発-" 東京大学教養学部体育学紀要. (印刷中). (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 相馬りか,宮下充正,八田英雄,跡見順子: "マウスの有酸素トレーニングによる乳酸の酸化の向上" 体力科学. 41. 206-212 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 跡見順子: "可塑性の鍵を握るプロテアーゼ" 運動生化学. (印刷中). (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Y.Atomi: "Decreased alphaB-crystallin in soleus muscle atrophy and role of aB-crystallin in muscle" Integration of Medical and Sport Sciences(Edited by Sato et al.). 37. 171-192 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Masuda, T.and Y.Atomi: "Fiber-type dependedt distribution of aB-crystallin in rat skeletal muscle" J.Appl.Physiol.(To be submitted).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Hatta, H.R.Soma, and Y.Atomi: "Effect of DCA administration or endurance training on lactate metabolism in mice during and after exercise" Ann.Physiol.9. 275-278 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Arai, H., M.Yamasita and Y.Atomi: "Response of Cultured Cell to stretch stimulation" Morphology and orientation Proc.Dept.Sports Sci.Univ.Tokyo. 28(in printing). (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Soma, R., H.Hatta, Y.Atomi, M.Miyashita: "The effect of aerobic training of lactate removal in mice during exercise" Ja.J.Sci.Physical Fitness. 41. 202-206 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Atomi, Y.: "Protease as a key of plasticity" Jap.J.Biochem Exercise. 8(in printing). (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Atomi,Y.,et al.: "Early Changes of αB-crystallin mRNA to mechanical tension and innervatin in rat skeletal muscle." Biochem.Biophys.Res.Commun.181. 1323-1330 (1991)

    • Related Report
      1992 Annual Research Report
  • [Publications] Atomi,Y.: "Decreased αB-Crystallin in Soleus Muscle Atrophy and Role of αB-Crystallin in Muscle." Med.Sport Sci.37. 171-192 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Atomi,Y.(Edited by Sato,Y.,J.Poortsman,I.Hashimoto,): "Integration of Medical and Sports Sciences." Karger,446 (1992)

    • Related Report
      1992 Annual Research Report

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Published: 1992-04-01   Modified: 2016-04-21  

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