| Project/Area Number |
08671997
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Otorhinolaryngology
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| Research Institution | Hyogo College of Medicine |
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Principal Investigator |
SEO Toru Hyogo College of Medicine, Instructor, 医学部, 助手 (30258149)
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| Co-Investigator(Kenkyū-buntansha) |
NOGUCHI Koichi Hyogo College of Medicine, Professor, 医学部, 教授 (10212127)
SATOMI Fumio Hyogo College of Medicine, Assistant Professor, 医学部, 講師 (20248149)
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| Project Period (FY) |
1996 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1997: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1996: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | peripheral facial nerve paralysis / in situ hybridization / CGRP / c-jun / GAP-43 / SOD / 神経伝達物質 / 免疫組織反応 |
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| Research Abstract |
It is well known that expression of CGRP (calcitonin gene-related peptide) , c-jun (immediate early gene) and GAP-43 (growth-associated protein, B-50) mRNAs increase in the rat facial nerve nuclei after peripheral nerve injury. Direct damage to the nerve is supposed to induce changes in gene expression of these neuroactive substances, which may have roles in adaptive response to the injury, neuronal survival, growth and regeneration. This study was designed to establish ischemic animal model of peripheral facial nerve paralysis with a selective embolization technique. In this model, the paralysis of facial continued for about 3 days and recovered without any neuronal deficit. We observed the levels in CGRP,c-jun and GAP-43 mRNAs in facial nerve nuclei using in situ hybridization. The increase of these mRNA appeared one day after embolization, lasted for 1 week and gradually decreased. The results suggest that the expression of CGRP,c-jun and GAP-43 in cell somata is induced by ischemic facial nerve paralysis, and the level and duration of increase in these mRNAs are different from those in direct nerve injury model.
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