| Project/Area Number |
12671979
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | MATSUMOTO DENTAL UNIVERSITY (2001)
Hyogo Medical University (2000) |
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Principal Investigator |
KONDO Eiji Matsumoto Dental University, Dept. of Oral Functional Anatomy, Associate Professor, 歯学部, 助教授 (50273636)
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| Co-Investigator(Kenkyū-buntansha) |
INOUE Katsuhiro Matsumoto Dental University, Dept. of Oral Functional Anatomy, Professor, 歯学部, 教授 (70018422)
IWATA Koichi Osaka University Graduate School, Facul. of Dent, Dept of Oral Physiology, Associate Professor, 大学院・歯学研究科, 助教授 (60160115)
NOGUCHI Koichi Hyogo College of Medicine, Dept. of Anatomy & Neuroscience, Professor, 医学部, 教授 (10212127)
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| Project Period (FY) |
2000 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 2001: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2000: ¥1,900,000 (Direct Cost: ¥1,900,000)
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| Keywords | gracile nucleus / neuropathic pain / chronic constriction injury / AMPA / in situ hybridization / NMPA / glutamate receptor / mRNA |
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| Research Abstract |
We examined change in gene expression in the rat nervous system with experimental mononeuropathy.
1) Pain model
We used Chronic Constriction Injury model and Chung model, Zeltzer model, Crush model. Allodynia and Hyperalgesia examined by Pain Behavior Test, von Frey filaments and Planter foot test. Furthermore, we used a rat model of facial paralysis.
2) Involvement of glutamate receptors on Neuropathic pain
In order to clarify the functional role of glutamate receptors on the gracile nucleus neurons involved in nerve injury-induced hyperalgesia, pharmacological, electro-physiological and in situ hybridization technic were rats with experimental mononeuropathy. CNQX, AMPA type receptor antagonists significantly depressed the responses to mechanical stimuli. The responses to noxious stimuli were much more strongly affected by CNQX as compared with those to innocuous stimuli. In contrast, we observed only slight modulation of neuronal activity of wide dynamic range neurons in the gracile nucleus following NMDA antagonists, MR-801 and AP-5, applications. These suggest that AMPA receptors would be dominantly involved in the hyperexcitability of WDR neurons in gracile nucleus, and may be related to the hyperalgesia observed following peripheral nerve injury.
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