| Project/Area Number |
13460143
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
生物資源科学
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| Research Institution | Hokkaido University |
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Principal Investigator |
HASHIDOKO Yasuyuki Hokkaido University, Hokkaido University, Graduate School of Agriculture, Associate Professor (40281795)
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| Co-Investigator(Kenkyū-buntansha) |
TAHARA Satoshi Hokkaido University, Graduate School of Agriculture, Professor (50001475)
HASEGAWA Toshihiro Hokkaido University, Graduate School of Agriculture, Associate Professor (10228455)
SHINANO Takuro 北海道大学, National Institute for Agro-Environmental Sciences, Group Leader (20235542)
FUKUSHI Yukiharu Hokkaido University, Graduate School of Agriculture, Lecturer (60218906)
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| Project Period (FY) |
2001 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥14,400,000 (Direct Cost: ¥14,400,000)
Fiscal Year 2003: ¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2002: ¥4,400,000 (Direct Cost: ¥4,400,000)
Fiscal Year 2001: ¥6,500,000 (Direct Cost: ¥6,500,000)
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| Keywords | acid sulfate soil-tolerant plants / rhizoplane bacteria / nitrogen fixing bacteria / nitrogen circulation / Sphingomonas sp. / rhizo biocomplex / functionality on the rhizoplane / nifgenes / 強酸性耐性 / 根面微生物 / 微生物フローラの多様性 / 単生窒素固定細菌 / 根面pHの上昇 / Sphingomonas / 根面バイオフィルム / 硫酸根の還元 / 微生物のフローラの多様性 / 耐酸性の獲得 |
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| Research Abstract |
In this study, we cultured rot washings in nitrogen-free, soft gel medium as a primary screening of functional rhizoplane and/or rhizosphere microbes, and we efficiently and selectively obtained α- and β-proteobacteria to exclude saprophytic γ-proteobacteria. Those oligotrophic and/or diazotrophic bacteria were visualized their behaviors in the soft gel medium, including their biocomplex formation and tripartite partnerships. The bacterial flora propagated in the N-free soft gel medium of gellan gum base survived relatively longer in the medium at room temperature due to a biofilm-like colonization and quorum-sensing like dormancy. Thus, we were able to trail new area of functional rhizo-biocomplex connected to the study of behabior of rhizoplane microfloral communities. As a demonstration, we have isolated and sub-cultured two rhizoplane bacteria, Sphingomonas rosa and Burkholderia cepacia, from rhizoplane of Melastoma malabathricum, a representative acid sulfate soil-tolerant plant, and identified them with 16S rRNA gene sequencing and data base search. The S. rosa showed. abilities to alter rhizospherous conditions and also to enhance nitrogen supply from the rhizo-biocomplex in a sotrongly acidic conditions. Like S. rosa, many other Sphingomonas spp. often isolated from the rhizoplane of acid-tolerant plants in Kalimantan had characteristics of 1) contributing to reliving divert soil in the rhizosphere, 2) fixing nitrogen in the rhizoplane or at least adjusting conditions for nitrogen fixation, and 3) being regulated the microfloral population via Mg^<2+> in the rhizosphere. In addition, Sphingomonas spp. had tight partnerships and tripartite-like co-works in the rhizoplane and/or inner tissues of host plants. Sphingomonas spp. are thus expected to be a motive force for nitrogen circulation and high genetic diversity in the rhizo-biocomplex.
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