| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
The neuronal growth cone is a motile tip of the growing axon at the times of the neuronal development/regeneration. The continuous reorganization of actin cytoskeleton and the membrane retrieval from the plasma membrane are observed in the leading edge of the growth cone. We found that the synaptophysin (Syp)-positive vesicles arose near the filopodia, and most of them were retrogradely moving along the actin bundles, using a superresolution microscopy 3D-SIM. The retrogradely moving Syp were colocalized with a BAR domain protein, endophilin A3 (Endo3), and dynamin 1 (Dnm1), but not with clathrin. Whereas clathrin mainly accumulated in the basal membrane in the central domain of the growth cone, Endo3 emerged in the dorsal surface. Accumulation of Endo3 depended on the F-actin bundling. These results suggest that the Syp-vesicles by Endo3-, Dnm1-dependent and clathrin-independent endocytosis, occurring at the apical membrane of the leading edge, coincides with filopodial formation.
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