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Analysis of proteins regulating GLUT4 translocation

Research Project

Project/Area Number 18390266
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Metabolomics
Research InstitutionTohoku University

Principal Investigator

KANZAKI Makoto  Tohoku University, 大学院医・工学研究科, 准教授 (10272262)

Co-Investigator(Kenkyū-buntansha) 根建 拓  東北大学, 先進医工学研究機構, 助教 (50375200)
藤田 英明  東北大学, 豊田中央研究所, 研究員 (50318804)
Project Period (FY) 2006 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥18,100,000 (Direct Cost: ¥15,400,000、Indirect Cost: ¥2,700,000)
Fiscal Year 2008: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2007: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
Fiscal Year 2006: ¥6,400,000 (Direct Cost: ¥6,400,000)
Keywordsインスリン / GLUT4 / 脂肪細胞 / 骨格筋細胞 / 細胞内小胞輸送 / 糖尿病 / 糖代謝 / 小胞輸送 / イメージング / シグナル伝達 / ナノ材料
Research Abstract

インスリン反応性GLUT4膜移行機構に関わる制御蛋白質の同定およびそれらの機能について、生化学的手法および生細胞のイメージング技術を組み合わせることにより解析した。GLUT4小胞のインスリン反応性獲得には、GLUT4に直接会合する3種類の蛋白(Sortilin-p75NTR-proNGF)による複合体形成とそれに伴うGLUT4の蛋白寿命制御が重要な役割を果たしていることを明らかにした。さらに、このGLUT4を含む蛋白複合体形成は、細胞内シグナル伝達系を活性化して積極的に筋分化にも関与することを明らかにした。

Report

(4 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • 2006 Annual Research Report
  • Research Products

    (52 results)

All 2009 2008 2007 2006

All Journal Article (16 results) (of which Peer Reviewed: 10 results) Presentation (19 results) Book (17 results)

  • [Journal Article] Contractile C2C12 myotube model for studying contraction-inducible responses of skeletal muscle (2008)2008

    • Author(s)
      Nedachi T, Fujita H, Kanzaki M
    • Journal Title

      Am J Physiol Endocrinol Metab 295 295(5)

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Functional role of sortilin in myogenesis and insulin-responsive glucose transport system in C2C12 myocytes2008

    • Author(s)
      Ariga M, Nedachi T, Katagiri H, Kanzaki M
    • Journal Title

      J Biol. Chem. 283(15)

      Pages: 10208-10220

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Ambient glucose levels qualify the potency of insulin myogenic actions by regulating SIRT1 and FoxO3a in C2C12 myocytes2008

    • Author(s)
      Nedachi T, Kadotani A, Ariga M., Katagiri H, Kanzaki M
    • Journal Title

      Am. J. Physiol Endocrinol Metab 294(4)

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Electric pulse stimulation induces NMDA glutamate receptor mRNA in NIH3T3 mouse fibroblasts2008

    • Author(s)
      Okutsu S, Hatakeyama H, Kanzaki M, Tsubokawa H, Nagatomi R.
    • Journal Title

      Tohoku J Exp Med. 215

      Pages: 181-187

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Contractile C2C12 myotube model for studying exercise-inducible responses in skeletal muscle2008

    • Author(s)
      Nedachi T
    • Journal Title

      American Journal of Physiology Endocrinology & Metabolism 295

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification of contraction-inducible myokines and their impact on insulin responsive GLUT4 translocation in C2C12 myotubes2008

    • Author(s)
      Nedachi T
    • Journal Title

      Diabetes 57 suppl

    • Related Report
      2008 Annual Research Report
  • [Journal Article] Exercising Cultured Myotubes2008

    • Author(s)
      Kanzaki M.
    • Journal Title

      Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology 151

      Pages: 443-443

    • Related Report
      2008 Annual Research Report
  • [Journal Article] Functional role of sortilin in myogenesis and insulin-responsive glucose transport system in C2C12 myocytes2008

    • Author(s)
      Ariga, M, et. al.
    • Journal Title

      Journal of Biological Chemistry 283

      Pages: 10208-10220

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Accelerated de novo sarcomere assembly by electric pulse stimulation in C2C12 myotubes2007

    • Author(s)
      Fujita H, Nedachi T, Kanzaki M
    • Journal Title

      Exp Cell Res 313(9)

      Pages: 1853-1865

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Involvement of apolipoprotein E in excess fat accumulation and insulin resistance2007

    • Author(s)
      Gao J. Katagiri H, Ishigaki Y, Yamada T, Ohihara T, Imai J, Uno K, Hasegawa Y, Kanzaki M, Yamamoto TT, Ishibashi S, Oka Y.
    • Journal Title

      Diabetes 56(1)

      Pages: 24-33

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Single molecule analysis of insulin-induced GLUT4 behavior in 3T3L1 adipocyte using Qdot nano-crystals2007

    • Author(s)
      Fujita H., et. al.
    • Journal Title

      Diabetes 55 (suppl.)

    • Related Report
      2007 Annual Research Report
  • [Journal Article] Functional role of sortilin in myogenesis and insulin-responsive glucose transport system in C2C12 myocytes2007

    • Author(s)
      Ariga M., et. al.
    • Journal Title

      Diabetes 55 (suppl.)

    • Related Report
      2007 Annual Research Report
  • [Journal Article] Involvement of apolipoprotein E in excess fat accumulation and insulin resistance2007

    • Author(s)
      Gao J., et al.
    • Journal Title

      Diabetes 56(1)

      Pages: 24-33

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Regulation of glucose transporters by insulin and extracellular glucose in C2C12 myotubes2006

    • Author(s)
      Nedachi T, Kanzaki M.
    • Journal Title

      Am. J. Physiol. Endocrinol & Metab 291

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Insulin receptor signals regulating GLUT4 translocation and actin dynamics2006

    • Author(s)
      Kanzaki M
    • Journal Title

      Endocrine Journal 53

      Pages: 267-293

    • NAID

      10018177532

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Insulin receptor signals regulating GLUT4 translocation and actin dynamics (Review Article)2006

    • Author(s)
      Kanzaki Makoto
    • Journal Title

      Endocrine Journal 53(3)

      Pages: 267-293

    • Related Report
      2006 Annual Research Report
  • [Presentation] 収縮筋細胞由来分泌因子群の発現制御とその役割2008

    • Author(s)
      神崎展
    • Organizer
      第12回Molecular Cardiovascular Conference
    • Place of Presentation
      小樽
    • Year and Date
      2008-09-05
    • Related Report
      2008 Annual Research Report
  • [Presentation] 培養筋細胞を運動させる2008

    • Author(s)
      神崎展
    • Organizer
      第30回日本比較生理生化学会シンポジウム「生物学と工学の接点」
    • Place of Presentation
      札幌
    • Year and Date
      2008-07-19
    • Related Report
      2008 Annual Research Report
  • [Presentation] Involvement of autocrine EGF-Receptor ligands in the palmitate-induced COX-2 expression in C2C12 myotubes2008

    • Author(s)
      Kadotani A., Katagiri H., Kanzaki M
    • Organizer
      48^<th> The American Society for Cell Biology Annual Meeting(アメリカ細胞生物学会)
    • Place of Presentation
      San Francisco, USA
    • Related Report
      2008 Final Research Report
  • [Presentation] Identification of contraction-inducible myokines and their impact on insulin responsive GLUT4 translocation in C2C12 myotubes2008

    • Author(s)
      Nedachi T
    • Organizer
      第68回アメリカ糖尿病学会
    • Place of Presentation
      San Francisco, CA
    • Related Report
      2008 Annual Research Report
  • [Presentation] Involvement of autocrine EGF-receptor ligands in the palmitate-induced COX-2 expression in C2C12 myotubes2008

    • Author(s)
      Kadotani A
    • Organizer
      第48回アメリカ細胞生物学会
    • Place of Presentation
      San Francisco, CA
    • Related Report
      2008 Annual Research Report
  • [Presentation] Functional role of sortilin in myogenesis and insulin-responsive glucose transport system in C2C12 myocytes2007

    • Author(s)
      Ariga M, et. al.
    • Organizer
      第67回アメリカ糖尿病学会
    • Place of Presentation
      Chicago, IL
    • Year and Date
      2007-06-24
    • Related Report
      2007 Annual Research Report
  • [Presentation] 蛍光ナノ粒子を用いたインスリン反応性GLUT4分子挙動変化の解析2007

    • Author(s)
      神崎 展
    • Organizer
      第50回日本糖尿病学会 シンポジウム「小胞輸送と糖代謝調節」
    • Place of Presentation
      仙台
    • Year and Date
      2007-05-25
    • Related Report
      2007 Annual Research Report
  • [Presentation] 収縮筋細胞内におけるGLUT4分子の挙動解析2007

    • Author(s)
      藤田英明・渡邊朋信・根建拓・樋口秀男・神崎展
    • Organizer
      第45回生物物理学会
    • Place of Presentation
      パシフィコ横浜(神奈川)
    • Related Report
      2008 Final Research Report
  • [Presentation] ナノ蛍光粒子を用いたインスリン反応性GLUT4分子挙動変化の解析2007

    • Author(s)
      神崎展
    • Organizer
      第50回日本糖尿病学会シンポジウム「小胞輸送と糖代謝調節」
    • Place of Presentation
      仙台
    • Related Report
      2008 Final Research Report
  • [Presentation] 展筋のインスリン反応性に対する運動効果の解析-収縮能を有する高度発達型培養筋細胞系の構築-2007

    • Author(s)
      根建拓・藤田英明・神崎展
    • Organizer
      第50回日本糖尿病学会
    • Place of Presentation
      仙台
    • Related Report
      2008 Final Research Report
  • [Presentation] 高度発達型培養筋細胞の作製技術とその糖尿病研究への利用2007

    • Author(s)
      根建拓・藤田英明・神崎展
    • Organizer
      第47回 生体医工学会
    • Place of Presentation
      仙台
    • Related Report
      2008 Final Research Report
  • [Presentation] Qdotを用いたGLUT4分子挙動解析-TIRF顕微鏡による膜融合過程の観察-2007

    • Author(s)
      藤田英明・渡邊朋信・根建拓・樋口秀男・神崎展
    • Organizer
      第47回生体医工学会
    • Place of Presentation
      仙台
    • Related Report
      2008 Final Research Report
  • [Presentation] Qdotを用いたGLUT4分子挙動解析-TIRF顕微鏡による膜融合過程の観察-2007

    • Author(s)
      藤田英明, ら
    • Organizer
      第47回日本生体医工学会
    • Place of Presentation
      仙台
    • Related Report
      2007 Annual Research Report
  • [Presentation] Single molecule analysis of insulin-induced GLUT4 behavior in 3T3L1 adipocyte using Qdot nano-crystals2007

    • Author(s)
      Fujita, et. al.
    • Organizer
      第67回アメリカ糖尿病学会
    • Place of Presentation
      Chicago, IL
    • Related Report
      2007 Annual Research Report
  • [Presentation] Live cell tracking of GLUT4 molecule in 3T3L1 adipocyte using Qdot nano-crystals2006

    • Author(s)
      Fujita H, Watanabe T, Nedachi, T, Higuchi,H, Kanzaki,M
    • Organizer
      46^<th> The American Society for Cell Biology Annual Meeting(アメリカ細胞生物学会)
    • Place of Presentation
      San Diago
    • Related Report
      2008 Final Research Report
  • [Presentation] Sortilin regulates myogenesis and insulin-induced GLUT4 translocation in C2C12 myocytes2006

    • Author(s)
      Ariga, M, Nedachi, T, Katagiri, H. Kanzaki,M
    • Organizer
      20^<th> International Congress of Biochemistry and Molecular Biology(第20回国際生化学・分子生物学会)
    • Place of Presentation
      Kyoto366(2P-C-145)
    • Related Report
      2008 Final Research Report
  • [Presentation] Degradation of Sir2 by ubiquitin-proteasome pathway that depends upon metabolic states controls C2C12 cell differentiation2006

    • Author(s)
      Nedachi,T, Kanzaki, M
    • Organizer
      20^<th> International Congress of Biochemistry and Molecular Biology(第20回国際生化学・分子生物学会)
    • Place of Presentation
      Kyoto539(4P-A-028)
    • Related Report
      2008 Final Research Report
  • [Presentation] Development of insulin-responsive GLUT4 trafficking machinery by forced Ca2+ transients in C2C12 myotubes2006

    • Author(s)
      Nedachi T, Fujita, H, Kanzaki, M
    • Organizer
      66^<th> American Diabetes Association Scientific Sessions第66回アメリカ糖尿病学会
    • Place of Presentation
      Washington DC(Diabetes55 : (suppl1) : A242 (1022-P))
    • Related Report
      2008 Final Research Report
  • [Presentation] 新規高度発達型培養筋細胞系におけるGLUT4膜移行を指標とした疑似運動刺激効果の検討2006

    • Author(s)
      根建拓・藤田英明・神崎展
    • Organizer
      第49回日本糖尿病学会年次学術集会
    • Place of Presentation
      東京
    • Related Report
      2008 Final Research Report
  • [Book] 特集「インスリン作用の原点」(江本政弘)2009

    • Author(s)
      神崎展
    • Publisher
      筋・脂肪におけるインスリン作用糖尿病(印刷中)
    • Related Report
      2008 Final Research Report
  • [Book] 筋肉におけるエネルギー代謝調節とインスリン抵抗性実験医学「エネルギー代謝研究の最前線」(岡芳知, 片桐秀樹)2009

    • Author(s)
      神崎展
    • Publisher
      羊土社(増刊号)
    • Related Report
      2008 Final Research Report
  • [Book] インスリン受容体シグナルとGLUT4小胞輸送系との接点-モーター蛋白質Myo1cの活性化-2009

    • Author(s)
      神崎展
    • Publisher
      実験医学
    • Related Report
      2008 Final Research Report
  • [Book] 実験医学・増刊号(共著)「筋肉におけるエネルギー代謝調節とインスリン抵抗性」「エネルギー代謝研究の最前線」監修 : 岡芳知・片桐秀樹2009

    • Author(s)
      神崎展
    • Publisher
      羊土社(印刷中)
    • Related Report
      2008 Annual Research Report
  • [Book] 「筋・脂肪におけるインスリン作用」特集 : 「インスリン作用の原鳥」編集 : 江本政弘2009

    • Author(s)
      神崎展
    • Publisher
      日本糖尿病学会(印刷中)
    • Related Report
      2008 Annual Research Report
  • [Book] Live cell イメージング技術を用いたインスリン反応性GLUT4トランスロケーションの解析分子糖尿病学の進歩2008(矢崎義雄)2008

    • Author(s)
      藤田英明・神崎展
    • Publisher
      金原出版
    • Related Report
      2008 Final Research Report
  • [Book] GLUT4小胞輸送とインスリン抵抗性新時代の糖尿病学12008

    • Author(s)
      有賀美也子・根建拓・神崎展
    • Publisher
      日本臨床社
    • Related Report
      2008 Final Research Report
  • [Book] 容量依存性カルシウム流入の分子基盤の解明2008

    • Author(s)
      神崎展
    • Publisher
      実験医学
    • Related Report
      2008 Final Research Report
  • [Book] PPARδとAMPキナーゼを活性化する薬剤は運動効果をもたらす2008

    • Author(s)
      神崎展
    • Publisher
      実験医学
    • Related Report
      2008 Final Research Report
  • [Book] リソソームにおけるClC-1の働きと酸性オルガネラの成熟2008

    • Author(s)
      神崎展
    • Publisher
      実験医学
    • Related Report
      2008 Final Research Report
  • [Book] mTORによるミトコンドリア機能の制御機構2008

    • Author(s)
      神崎展
    • Publisher
      実験医学
    • Related Report
      2008 Final Research Report
  • [Book] 分子糖尿病学の進歩2008 (共著)「Live cellイメージング技術を用いたインスリン反応性GLUT4トランスロケーションの解析2008

    • Author(s)
      藤田英明, ら
    • Publisher
      金原出版
    • Related Report
      2007 Annual Research Report
  • [Book] 新時代の糖尿病学(共著)「GLUT4小胞輸送とインスリン抵抗性」2008

    • Author(s)
      有賀美也子, ら
    • Publisher
      日本臨床社
    • Related Report
      2007 Annual Research Report
  • [Book] 脂質膜の構造変形に関わるMechano-enzyme EHDs2007

    • Author(s)
      神崎展
    • Publisher
      実験医学
    • Related Report
      2008 Final Research Report
  • [Book] SUMO化修飾によるカイニン酸受容体チャネルのエンドサイトーシス2007

    • Author(s)
      神崎展
    • Publisher
      実験医学
    • Related Report
      2008 Final Research Report
  • [Book] 脂肪細胞の肥大とマトリックスメタロプロテアーゼ2006

    • Author(s)
      神崎展
    • Publisher
      実験医学
    • Related Report
      2008 Final Research Report
  • [Book] pHセンサーとしてのV-ATPaseの役割と細胞内小胞輸送制御2006

    • Author(s)
      神崎展
    • Publisher
      実験医学
    • Related Report
      2008 Final Research Report

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Published: 2006-04-01   Modified: 2016-04-21  

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