In vivo patch-clamp analysis of developmental changes in the spinal nociceptive circuit.
| Project/Area Number |
18613009
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pain science
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| Research Institution | Kyushu University |
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Principal Investigator |
FURUE Hidemasa Kyushu University, Graduate School of Medical Sciences, Assistant Professor (20304884)
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| Co-Investigator(Kenkyū-buntansha) |
YOSHIMURA Megumu Kyushu University, Graduate School of Medical Sciences, Professor (10140641)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥4,090,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥390,000)
Fiscal Year 2007: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2006: ¥2,400,000 (Direct Cost: ¥2,400,000)
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| Keywords | synaptic transmission / nrimary afferent / spinal cord / neuronal circuit / C fiber / electrophysiology / neuron / mechanoreceptor / パッチクランプ / in vivo / 感覚 / 痛覚伝達 / 電気生理 / 脊髄 |
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| Research Abstract |
Developmental changes in the spinal nociceptive synaptic transmission were examined using slice and in vivo patch-clamp recordings. In transverse spinal cord slices of young and adult rats at lumbar spinal levels with an attached dorsal root, electrical dorsal root stimulation was applied to record the evoked-synaptic responses in substantia gelatinosa (SG, lamina II of the spinal cord)and deep lamina (lamina III-IV) neurons. In the in vivo patch-clamp analysis, patch electrodes were inserted into the SG at the spinal level of L3-L5 after a thoracolumbar laminectomy was performed under urethane anesthesia. Whole-cell recordings were obtained from SG neurons, and then modality-dependent synaptic responses evoked by cutaneous natural mechanical stimuli applied to the ipsilateral hind limb were recorded. In adult rats, monosynaptic Aβ and C fiber-evoked EPSCs were elicited in SG neurons. Additionally, monosynapitc Aβ fiber-evoked EPSCs were evoked in the deeper lamina neurons but not in SG neurons. In young rats, on the other hand, monosynaptic Aβ fiber-evoked EPSCs were observed in SG neurons in addition to Aβ and C fiber-evoked responses. In the in vivo patch-clamp analysis, pinch stimulation elicited a barrage of EPSCs in SG neurons in young and adult rats. Even in SG neurons that received synaptic inputs from only Aβ fibers, pinch stimulation evoked a barrage of EPSCs. Most deep neurons in adult rats responded to touch stimulation. The present results suggest that Aβ fibers, in addition to Aδ and C fibers, convey noxious mechanical information to SG neurons in young rats. In adult rats with further development of neuronal circuits in the spinal dorsal horn, innocuous mechanical synaptic responses are mediated only through Aβ fibers in deep lamina neurons.
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Report
(3 results)
Research Products
(127 results)
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[Journal Article] Cadherin-8 is Required for the First Relay Synapses to Receive Functional Inputs from Primary Sensory Afferents for Cold Sensation2007
Author(s)
Fume, H., Suzuki,SC.,Koga, K., Jiang, N., Nohmi,M., Shimazaki,Y., Katoh-Fukui,Y., Yokoyama,M., Yoshimura, M.,Takeichi M.
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Journal Title
Journal of Neuroscience 27
Pages: 3466-3476
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Cell type specific excitatory and inhibitory circuits involving primary afferents in the substantia gelatinosa of the rat spinal dorsal horn in vitro.2007
Author(s)
Yasaka,T., Kato, G., Fume,H., Rashid,MH., Sonohata,M., Tamae,A., Murata,Y., Masriko,S., Yoshimura M.
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Journal Title
Journal of Physiology(London) 581(2)
Pages: 603-618
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Slow oscillation of membrane currents mediated by glutamatergic inputs of rat somatosensory cortical neurons : in vivo patch-clamp analysis2007
Author(s)
Doi,A., Mizuno, M.,Katafuchi,T., Fume, H., Koga, K., YoshimuraM.
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Journal Title
European Journal of Neuroscience 26(9)
Pages: 2565-2575
Description
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[Journal Article] Reduced pain behaviors and extracellular signal-related protein kinase activation in primary sensory neurons by peripheral tissue injury in mice lacking platelet-activating factor receptor2007
Author(s)
Tsuda, M., Ishii,S., Masuda,T., Hasegawa,S., K., Nakamura, Nagata, K., Yamashita,T., Fume,H., Tozaki-Saitoh,H., Yoshimura,M., Koizumi, S., Shimizu, K., Inoue T
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Journal Title
Journal of Neurochemistry 102(5)
Pages: 1658-1668
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] In vivo patch-clamp technique.2007
Author(s)
H., Fume, T., Katafuchi, M., Yoshimura
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Journal Title
Patch-Clamp Analysis : advanced techniques, Second Edition. Ed.(W., Walz)(Humana Press Inc., Totowa)
Pages: 229-251
Description
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[Journal Article] Direct excitation of deep dorsal horn neurones in the rat spinal cord by the activation of postsynaptic P2X receptors2006
Author(s)
Shiokawa,H., Nakatsuka, T., Furue, H., Tsuda,M., Katafuchi,T., Inoue, K., Yoshimura M.
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Journal Title
Journal of Physiology(London) 573(Pt3)
Pages: 753-763
Description
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[Presentation] Modification of bursting activities in somatosensory cortex in inflamed -rats in vivo.2007
Author(s)
K., Koga, H., Shiokawa, M., Mizuno, A., Doi, H., Takase-Mizuguchi, H., Fume, M., Yoshimura
Organizer
Second International Congress on Neuropathic Pain
Place of Presentation
Berlin, Germany
Description
「研究成果報告書概要(欧文)」より
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[Presentation] In vivo and slice patch-clamp analyses of plastic changes in descending inhibition of spinal noxious mechanical synaptic transmission in ovariectomized rats.2007
Author(s)
H., Fume, Y., Omori, A., Ito, Y., Kuraishi, M., Yoshimura
Organizer
The 37th Annual Meeting of the Society for Neuroscience
Place of Presentation
San Diego, USA
Description
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[Presentation] Electrophysiological and morphological analyzes of excitatory effect of TRPA1 agonists on synaptic transmission in substantia gelatinosa neurons of the adult rat spinal cord.2007
Author(s)
D., Uta, MH., Rashid, H., Fume, R., Hisamitsu, M., Yoshimura
Organizer
The 37th Annual Meeting of the Society for Neuroscience
Place of Presentation
San Diego, USA
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Cortical membrane potential fluctuations may be modified by thalamocortical inputs in rat barrel cortex in vivo.2007
Author(s)
M., Mizuno, A., Doi, T., Yasaka, H., Fume, S., Aou, M., Yoshimura
Organizer
The 37th Annual Meeting of the Society for Neuroscience
Place of Presentation
San Diego, USA
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Reduction of menthol-sensitive afferent inputs to superficial dorsa neurons in knockout mice of cadherin-8.2007
Author(s)
H., Fume, S., Suzuki, K., Koga, Y., Shimazaki, M., Nohmi, M., Takeichi, M., Yoshimura
Organizer
The 84th Annual Meeting of the Physiological Society of Japan
Place of Presentation
Osaka, Japan
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Analogy of A-beta afferent sprouting in inflamed rats with sensory circuit in developing spinal dorsal horn.2007
Author(s)
M., Yoshimura, H., Fume, S., Matayoshi, T., Nakatsuka, T., Katafuchi
Organizer
The 84th Annual Meeting of the Physiological Society of Japan
Place of Presentation
Osaka, Japan
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Functional role pf GABA receptor and its modification in the spinal dorsal horn.2007
Author(s)
M., Yoshimura, T., Takazawa, T., Kohno, H., Fume, K., Koga
Organizer
The 54th Annual Meeting of Japanese Society of Anesthesiologists
Place of Presentation
Sapporo, Japan
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Impairment of afferent synaptic inputs for mechanical and cold sensation to substantia gelatinosa neurons of the rat spinal dorsal horn through cadherin-8 expressing synaptic glomeruli.2007
Author(s)
H., Fume, SC., Suzuki, K., Koga, M., Nohmi, M., Takeichi, M., Yoshimura
Organizer
The 30th Annual Meeting of the Japan Neuroscience Society
Place of Presentation
Yokohama, Japan
Description
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[Presentation] In vivo patch-clamp analysis of plastic changes in descending serotonergic inhibition of noxious mechanical transmission to substantia gelatinosa neurons through 5-HT1A receptors in ovariectomized rats.2007
Author(s)
Y., Omori, H., Fume, A., Ito, Y., Kuraishi, M., Yoshimura
Organizer
The 30th Annual Meeting of the Japan Neuroscience Society
Place of Presentation
Yokohama, Japan
Description
「研究成果報告書概要(欧文)」より
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[Presentation] In vivo patch clamp analysis of synaptic responses evoked in primary somatosensory cortex in inflamed rats.2007
Author(s)
K., Koga, H., Shiokawa, M., Mizuno, A., Doi, H., Takase-Mizuguchi, H., Fume, M., Yoshimura
Organizer
The 30th Annual Meeting of the Japan Neuroscience Society
Place of Presentation
Yokohama, Japan
Description
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[Presentation] Behavioral and electrophysiological analyses of spinal nociceptive transmission in a murine model of cancer-induced bone pain.2007
Author(s)
Y., Yanagisawa, H., Fume, T., Kawamata, A., Namiki, Y., Iwamoto, M., Yoshimura
Organizer
The 22nd Annual Research Meeting of the Japanese Orthopaedic Association
Place of Presentation
Shizuoka, Japan
Description
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[Presentation] Central sensitization induced by switching of subunit in AMPA receptor following inflammatory pain.2007
Author(s)
T., Katano, H., Fume, M., Tagatani, E., Ashitaka, M., Yoshimura, S., Ito
Organizer
The 30th Annual Meeting of the Molecular Biology Society of Japan and The 80th Annual Meeting of the Japanese Biochemical Society
Place of Presentation
Yokohama, Japan
Description
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[Presentation] Functional loss of synaptic inputs to substantia gelatinosa neurons in the spinal cord through menthol-sensitive afferent fibers in cadherin-8 deficient mice.2006
Author(s)
Fume, H., Suzuki, S., Koga, K., Jiang, N., Nohmi, M., Takeichi, M., Yoshimura, M.
Organizer
The 36th Annual Meeting of Society for Neuroscience
Place of Presentation
Atlanta, USA
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Cadherin-8 regulates activities of the first relay synapse for cool sensation.2006
Author(s)
Suzuki, H., Fume, H., Koga, K., Jiang, N., Nohmi, M., Yoshimura, M., Takeichi, M
Organizer
The 36th Annual Meeting of Society for Neuroscience
Place of Presentation
Atlanta, USA
Description
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[Presentation] Actions of agonists for the noxious cold ion channel TRPAI in synaptic transmission in substantia gelatinosa neurons of the adult rat spinal cord.2006
Author(s)
Uta, D., Rashid, MH., Furue, H., Katafuchi, T., Hisamitsu, R., Shimada, H., Yoshimura, M
Organizer
The 36th Annual Meeting of Society for Neuroscience
Place of Presentation
Atlanta, USA
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Whole-cell patch-clamp analysis of firing patterns of ventral horn neurons in young adult rat in vitro.2006
Author(s)
Hisamitsu, R., Shimada, H., Uta, D., Take, S., Fume, H., Yoshimura, M
Organizer
The 36th Annual Meeting of Society for Neuroscience
Place of Presentation
Atlanta, USA
Description
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[Presentation] Physiological properties of substantia gelatinosa neurons in neonatal rats by in vivo patch-clamp analysis.2006
Author(s)
Kawamata, M., Sugino, S., Kiya, T., Fume, H., Yoshimura, M., Namiki, A
Organizer
The 28th Annual Meeting of JASP
Place of Presentation
Kobe, Japan
Description
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[Presentation] Actions of serotonin on nociceptive transmission in the substantia gelatinosa of the rat spinla cord.2006
Author(s)
Abe, K., Fume, H., Koga, K., Kato, G., Yasaka, T., Tamae, A., Katafuchi, T., Yoshimura, M
Organizer
The 29th Annual Meeting of the Japan Neuroscience Society
Place of Presentation
Kyoto, Japan
Description
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