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Research of microbe in the deepest subsurface biosphere based on RNA analysis

Research Project

Project/Area Number 20810047
Research Category

Grant-in-Aid for Young Scientists (Start-up)

Allocation TypeSingle-year Grants
Research Field Applied genomics
Research InstitutionJapan Agency for Marine-Earth Science and Technology

Principal Investigator

HORI Sayaka  Japan Agency for Marine-Earth Science and Technology, 医学部, 助教 (20470122)

堀 沙耶香 (2009)  Tokyo Women's Medical University, 医学部, 助教 (20810047)

Project Period (FY) 2007 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥3,302,000 (Direct Cost: ¥2,540,000、Indirect Cost: ¥762,000)
Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2008: ¥1,742,000 (Direct Cost: ¥1,340,000、Indirect Cost: ¥402,000)
Keywords微生物生態系 / 有孔虫 / 深海 / RNA / 極限環境 / 微生物 / 生態系 / 遺伝子発現 / バクテリア / 群集・生態系多様性 / ゲノム機能・発現 / 微生物ゲノム / 遺伝子多様性 / 環境徽生物 / 分子生物学
Research Abstract

To clarify bacterial diversity in the geological static deep-sea sediment, I tried to extract RNA to make cDNA libraries. The Automatic Bottom Inspection and Sampling Mobile "ABISMO" succeeded sampling at the deepest Mariana Trench, however, the included microbe was lower amount than previous report. Then, preliminary experiment was performed using the sediment in the static Izu-Ogasawara Trench. The population of Chloroflexi surrounding giant protist was significantly lower than the plain sediment, suggesting that bacteria in the activity state were distributed heterogeneously in the geologically static environment by coexistence with the large-sized creature.

Report

(3 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • Research Products

    (3 results)

All 2009 Other

All Presentation (2 results) Remarks (1 results)

  • [Presentation] Inactivation of alfa-proteobacteria and activation of gamma-proteobacteria induced by a new candidate genus Xenophyophores (giant foraminifera) at a depth 7111m in the Izu-Ogasawara Trench2009

    • Author(s)
      堀沙耶香, 土屋正史, 西真郎, 荒井渉, 吉田尊雄, 高見英人
    • Organizer
      第32回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2009-12-11
    • Related Report
      2009 Annual Research Report 2009 Final Research Report
  • [Presentation] Bacterial diversity surrounding a new candidate genes Xenophyophores (Foraminifera) discovered at a depth 7111m near the Boso Peninsula2009

    • Author(s)
      Sayaka Hori, Tsuchiya Masashi, Shinro Nishi, Wataru Arai, Takao Yoshida, , Hideto Takami
    • Organizer
      4th International Symposium on Chemosynthesis-Based Ecosystems
    • Place of Presentation
      沖縄
    • Year and Date
      2009-07-02
    • Related Report
      2009 Annual Research Report 2009 Final Research Report
  • [Remarks]

    • URL

      http://jglobal.jst.go.jp/public/20090422/200901016725047918

    • Related Report
      2009 Final Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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