| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Research Abstract |
In this project, we have studied how phototaxis of Chlamydomonas is redox-regulated. To clarify the molecular basis of this regulatory pathway, we took three strategies as follows:1) estimation of relationship between state transitions and phototactic signs; 2) identification of responsible genes of three mutants that have defects in redox sensitivity and phototaxis; and 3) identification of proteins that react with thioredoxins that function as dynein subunits. Results are 1) positively phototactic cells are in state 1, whereas negatively phototactic cells are in state 2; 2) one of the mutants has defects in a biosynyhesis pathway of a kind of pigment; and 3) proteins were captured by mutagenized thioredoxins in vivo, although identification of those proteins have not been finished. By further analyses of these results, the molecular mechanism of the regulatory pathway from photoreception to phototaxis will be clarified.
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