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Real-time imaging of excitation-secretioncoupling process from the single peptidergic neuron

Research Project

Project/Area Number 23770072
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Animal physiology/Animal behavior
Research InstitutionNagoya University (2012)
The University of Tokyo (2011)

Principal Investigator

ABE Hideki  名古屋大学, 大学院・生命農学研究科, 准教授 (90396804)

Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Keywords神経生物 / ペプチド放出 / GnRH / 開口放出 / 興奮分泌連関 / 細胞培養 / 神経修飾 / 相互結合 / ペプチド
Research Abstract

To understand fine tuning mechanisms of the central nervous system by peptidergic neurons, we investigated the excitation-secretion relationship of single peptidergic neuron using a primary culture system of isolated fish terminal nerve (TN)-gonadotropin releasing hormone (GnRH) neuron and gene transfer of exogenous genes by single cell electroporation. As a result, we enabled measurement of the movement of secretory vesicles and their exocytotic events from the subcellular region of single peptidergic neuron. We also analyzed the ionic mechanisms of the firing pattern control of TN-GnRH neurons that are the origin of exocytosis of GnRH peptide.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (55 results)

All 2013 2012 2011 Other

All Journal Article (7 results) (of which Peer Reviewed: 7 results) Presentation (42 results) Remarks (6 results)

  • [Journal Article] Neuropeptide RFRP inhibits the pacemaker activity of terminal nerve GnRH neurons2013

    • Author(s)
      Umatani, C., Abe, H., Oka, Y
    • Journal Title

      J. Neurophysiol

      Volume: 109 Pages: 2354-2363

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Time-of-Day-dependent changes in GnRH1 neuronal activities and gonadotropin mRNA expression in a daily spawning fish, medaka2012

    • Author(s)
      Karigo, T., Kanda, S., Takahashi, A., Abe, H., Okubo, K., Oka, Y
    • Journal Title

      Endocrinology

      Volume: 153 Pages: 3394-3404

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Dopaminergic neuromodulation of synaptic transmission between the mitral and granule cells in the teleost olfactory bulb2012

    • Author(s)
      Kawai, T., Abe, H., and Oka, Y
    • Journal Title

      J. Neurophysiol

      Volume: 107 Pages: 313-1324

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Time-of-day dependent changes in GnRH1 neuronal activities and gonadotropin mRNA expression in a daily spawning fish, medaka2012

    • Author(s)
      Karigo, T., Kanda, S., Abe, H., Okubo, K., and Oka, Y.
    • Journal Title

      Endocrinology

      Volume: 153 Issue: 7 Pages: 3394-3404

    • DOI

      10.1210/en.2011-2022

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Dopaminergic neuromodulation of synaptic transmission between mitral and granule cells in the teleost olfactory bulb2012

    • Author(s)
      Kawai T, Abe H, Oka Y
    • Journal Title

      Journal of Neurophysiology

      Volume: 107(5) Issue: 5 Pages: 1313-1324

    • DOI

      10.1152/jn.00536.2011

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Mechanisms of neuromodulation by a non-hypophysiotropic GnRH system controlling motivation of reproductive behavior in the teleost brain2011

    • Author(s)
      Abe, H. and Oka, Y
    • Journal Title

      J. Reprod. and Devel

      Volume: 57 Pages: 665-674

    • NAID

      10030405993

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Mechanisms of Neuromodulation by a Nonhypophysiotropic GnRH System Controlling Motivation of Reproductive Behavior in the Teleost Brain2011

    • Author(s)
      Hideki Abe and Yoshitaka Oka
    • Journal Title

      Journal of Reproduction and Development

      Volume: 57 Issue: 6 Pages: 665-674

    • DOI

      10.1262/jrd.11-055E

    • NAID

      10030405993

    • ISSN
      0916-8818, 1348-4400
    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] 遺伝子改変メダカを用いた黄体形成ホルモン(LH)放出の中枢制御機構の解析2013

    • Author(s)
      近藤千香,苅郷友美,相川雅人,神田真司,赤染康久,大久保範聡,阿部秀樹,岡良隆
    • Organizer
      日本動物学会関東支部第65 会大会
    • Place of Presentation
      東京工業大学
    • Related Report
      2012 Final Research Report
  • [Presentation] eural mechanisms of female sexual preference in medaka2012

    • Author(s)
      Okuyama, T., Yokoi, S., Abe, H., Taniguchi, Y., Okubo, K., Kamei, Y., Shimada, A., Naruse, K., Takeda, H., Oka, Y., Kubo, T., Takeuchi, H
    • Organizer
      2nd Annual Meetings
    • Place of Presentation
      Society for Neuroscience, New Orleans, USA
    • Related Report
      2012 Final Research Report
  • [Presentation] Neuropeptide RFRP inhibits the pacemaker activity of terminal nerve GnRH neurons2012

    • Author(s)
      Umatani, C., Abe, H., Oka, Y
    • Organizer
      42nd Annual Meetings, Society for Neuroscience
    • Place of Presentation
      New Orleans, USA
    • Related Report
      2012 Annual Research Report 2012 Final Research Report
  • [Presentation] Neuro-neuronal connections among the cluster of terminal nerve Gonadotropin Releasing Hormone (GnRH) peptidergic neurons: A model for studying the synchronization of peptidergic neurons2012

    • Author(s)
      Abe, H., Nishikawa, H., Suenami, S., Oka, Y
    • Organizer
      42nd Annual Meetings, Society for Neuroscience
    • Place of Presentation
      New Orleans, USA
    • Related Report
      2012 Annual Research Report 2012 Final Research Report
  • [Presentation] 終神経 GnRHニューロンにおけるバースト活動の誘起2012

    • Author(s)
      河合喬文・阿部秀樹・岡良隆
    • Organizer
      第105回近畿生理学談話会
    • Place of Presentation
      関西医科大学
    • Related Report
      2012 Final Research Report
  • [Presentation] Neural mechanisms of female sexual preference in medaka, Oryzias latipes (メダカにおける、メスの配偶者選択行動の神経機構)2012

    • Author(s)
      奥山輝大, 横井佐織, 阿部秀樹, 末廣勇司, 今田はるか, 田中実, 川崎隆史, 谷口善仁, 亀井保博, 島田敦子, 成瀬清, 武田洋幸, 岡良隆, 久保健雄, 竹内秀明
    • Organizer
      第35回日本神経科学大会
    • Place of Presentation
      名古屋
    • Related Report
      2012 Final Research Report
  • [Presentation] Neuropeptide RFRP inhibits the pacemaker activity of terminal nerve GnRH neurons (RFRP による終神経 GnRHニューロン発火活動抑制作用の解析)2012

    • Author(s)
      馬谷千恵・阿部秀樹・岡良隆
    • Organizer
      第35回日本神経科学大会
    • Place of Presentation
      名古屋
    • Related Report
      2012 Final Research Report
  • [Presentation] Burst induction mediated by cholinergic input in terminal nerve -gonadotropin releasing hormone neurons (終神経 GnRHニューロンにおけるコリン作動性入力を介したバースト活動の誘起)2012

    • Author(s)
      河合喬文・阿部秀樹・岡良隆
    • Organizer
      第35回日本神経科学大会
    • Place of Presentation
      名古屋
    • Related Report
      2012 Final Research Report
  • [Presentation] Neuro-neuronal connections among the cluster of terminal nerve GnRH peptidergic neurons (終神経 GnRH ペプチドニューロン細胞塊における GnRH ニューロン間結合様式)2012

    • Author(s)
      阿部秀樹, 西川穂高, 末次翔太, 岡良隆
    • Organizer
      第35回日本神経科学大会
    • Place of Presentation
      名古屋
    • Related Report
      2012 Final Research Report
  • [Presentation] Neural mechanisms of female sexual preference in medaka2012

    • Author(s)
      Okuyama, T., Yokoi, S., Abe, H., Taniguchi, Y., Okubo, K., Kamei, Y., Shimada, A., Naruse, K., Takeda, H., Oka, Y., Kubo, T., Takeuchi, H.
    • Organizer
      42nd Annual Meetings, Society for Neuroscience
    • Place of Presentation
      New Orleans, USA
    • Related Report
      2012 Annual Research Report
  • [Presentation] Neuro-neuronal connections among the cluster of terminal nerve GnRH peptidergic neurons2012

    • Author(s)
      阿部秀樹, 西川穂高, 末次翔太, 岡良隆
    • Organizer
      第35回日本神経科学大会
    • Place of Presentation
      名古屋
    • Related Report
      2012 Annual Research Report
  • [Presentation] Burst induction mediated by cholinergic input in terminal nerve -gonadotropin releasing hormone neurons2012

    • Author(s)
      河合喬文、阿部秀樹、岡良隆
    • Organizer
      第35回日本神経科学大会
    • Place of Presentation
      名古屋
    • Related Report
      2012 Annual Research Report
  • [Presentation] Neuropeptide RFRP inhibits the pacemaker activity of terminal nerve GnRH neurons2012

    • Author(s)
      馬谷千恵、阿部秀樹、岡良隆
    • Organizer
      第35回日本神経科学大会
    • Place of Presentation
      名古屋
    • Related Report
      2012 Annual Research Report
  • [Presentation] Neural mechanisms of female sexual preference in medaka, Oryzias latipes2012

    • Author(s)
      奥山輝大, 横井佐織, 阿部秀樹, 末廣勇司, 今田はるか, 田中実, 川崎隆史, 谷口善仁, 亀井保博, 島田敦子, 成瀬清, 武田洋幸, 岡良隆, 久保健雄, 竹内秀明
    • Organizer
      第35回日本神経科学大会
    • Place of Presentation
      名古屋
    • Related Report
      2012 Annual Research Report
  • [Presentation] キンギョ嗅球におけるドーパミンによる神経修飾作用2011

    • Author(s)
      河合喬文・阿部秀樹・岡良隆
    • Organizer
      第34回日本神経科学大会
    • Place of Presentation
      パシフィコ横浜
    • Related Report
      2012 Final Research Report
  • [Presentation] メダカの性選択の神経基盤2011

    • Author(s)
      竹内秀明、横井佐織、末廣勇司、今田はるか、田中実、川崎隆史、島田敦子、阿部秀樹、成瀬清、武田洋幸、久保健雄、奥山輝大
    • Organizer
      第34回日本神経科学大会
    • Place of Presentation
      パシフィコ横浜
    • Related Report
      2012 Final Research Report
  • [Presentation] メダカにおける視覚情報依存の配偶者選択行動の神経基盤2011

    • Author(s)
      奥山輝大、末廣勇司、今田はるか、阿部秀樹、島田敦子、田中実、成瀬清、武田洋幸、岡良隆、久保健雄、竹内秀明
    • Organizer
      第34回日本神経科学大会
    • Place of Presentation
      パシフィコ横浜
    • Related Report
      2012 Final Research Report
  • [Presentation] 小型魚類を用いた視覚情報依存的な配偶者選択行動の分子・神経基盤解析2011

    • Author(s)
      奥山輝大, 阿部秀樹, 末廣勇司, 今田はるか, 島田敦子, 川崎隆史, 弓場俊輔, 谷口善仁, 亀井保博, 田中実, 成瀬清, 武田洋幸, 岡良隆, 久保健雄, 竹内秀明
    • Organizer
      第34回 日本分子生物学会年会
    • Place of Presentation
      横浜
    • Related Report
      2012 Final Research Report
  • [Presentation] Analysis of the molecular and neural mechanism of female mating acceptance depending on visual information in small fish medaka2011

    • Author(s)
      Okuyama, T., Suehiro, Y., Imada, H., Shimada, A., Abe, H., Tanaka, M., Naruse, K., Takeda, H., Oka, Y., Kubo, Y., and Takeuchi, H
    • Organizer
      1st medaka strategic meeting
    • Place of Presentation
      Okazaki, JAPAN
    • Related Report
      2012 Final Research Report
  • [Presentation] Functional role of GnRH receptors for neuromodulation in the goldfish olfactory bulb2011

    • Author(s)
      Kawai, T., Kanda, S., AkazomeY, Abe, H., and Oka Y
    • Organizer
      41st Annual Meetings, Society for Neuroscience
    • Place of Presentation
      Washington DC, USA
    • Related Report
      2012 Final Research Report
  • [Presentation] Analysis of regulatory mechanisms of gonadotropin secretion using transgenic medaka (Oryzias latipes)2011

    • Author(s)
      Karigo, T., Aikawa, M., Kanda, S., Abe, H., Akazome,Y. Okubo, Y., and Oka, Y.
    • Organizer
      41st Annual Meetings, Society for Neuroscience
    • Place of Presentation
      Washington DC, USA
    • Related Report
      2012 Final Research Report
  • [Presentation] Analysis of the molecular and neural mechanism of female mating acceptance depending on visual information in small fish medaka2011

    • Author(s)
      Okuyama, T., Suehiro, Y., Imada, H., Shimada, A., Abe, H., Tanaka, M., Naruse, K., Takeda, H., Oka, Y., Kubo, Y., and Takeuchi, H
    • Organizer
      41st Annual Meetings, Society for Neuroscience
    • Place of Presentation
      Washington DC, USA
    • Related Report
      2012 Final Research Report
  • [Presentation] トランスジェニックメダカを用いた kiss1 ニューロンの電気生理学的解析2011

    • Author(s)
      島田洋之・神田真司・赤染康久・大久保範聡・阿部秀樹・岡良隆
    • Organizer
      第82回日本動物学会大会
    • Place of Presentation
      旭川
    • Related Report
      2012 Final Research Report
  • [Presentation] 脳内ペプチド RFRP による終神経 GnRH ニューロン抑制作用の解析2011

    • Author(s)
      馬谷千恵・阿部秀樹・岡良隆
    • Organizer
      第82回日本動物学会大会
    • Place of Presentation
      旭川
    • Related Report
      2012 Final Research Report
  • [Presentation] キンギョ嗅球におけるドーパミンによる神経修飾作用2011

    • Author(s)
      河合喬文・阿部秀樹・岡良隆
    • Organizer
      第82回日本動物学会大会
    • Place of Presentation
      旭川
    • Related Report
      2012 Final Research Report
  • [Presentation] ドワーフグラミー終神経 GnRH ニューロン細胞塊における GnRH ニューロン間結合様式2011

    • Author(s)
      阿部秀樹・西川穂高・岡良隆
    • Organizer
      第82回日本動物学会大会
    • Place of Presentation
      旭川
    • Related Report
      2012 Final Research Report
  • [Presentation] Analysis of the molecular and neural mechanism of female sexual preference that depends on visual information in small fish medaka2011

    • Author(s)
      Okuyama, T., Abe, H., Suehiro, Y., Imada, H., Shimada, A., Kawasaki, T., Yuba, S., Taniguchi, Y., Kamei, Y., Tanaka, M., Naruse, K., Takeda, H., Oka, Y., Kubo, Y., and Takeuchi, H
    • Organizer
      第17回小型魚類研究会
    • Place of Presentation
      三島
    • Related Report
      2012 Final Research Report
  • [Presentation] メダカの性選択の神経基盤2011

    • Author(s)
      竹内秀明、横井佐織、末廣勇司、今田はるか、田中実、川崎隆史、島田敦子、阿部秀樹、成瀬清、武田洋幸、久保健雄、奥山輝大
    • Organizer
      第34回日本神経科学大会
    • Place of Presentation
      神奈川県横浜市
    • Related Report
      2011 Research-status Report
  • [Presentation] メダカにおける視覚情報依存の配偶者選択行動の神経基盤2011

    • Author(s)
      奥山輝大、末廣勇司、今田はるか、阿部秀樹、島田敦子、田中実、成瀬清、武田洋幸、岡良隆、久保健雄、竹内秀明
    • Organizer
      第34回日本神経科学大会
    • Place of Presentation
      神奈川県横浜市
    • Related Report
      2011 Research-status Report
  • [Presentation] キンギョ嗅球におけるドーパミンによる神経修飾作用2011

    • Author(s)
      河合喬文、阿部秀樹、岡良隆
    • Organizer
      第34回日本神経科学大会
    • Place of Presentation
      神奈川県横浜市
    • Related Report
      2011 Research-status Report
  • [Presentation] 脳内ペプチドRFRPによる終神経GnRHニューロン抑制作用の解析2011

    • Author(s)
      馬谷千恵、阿部秀樹、岡良隆
    • Organizer
      第82回日本動物学会大会
    • Place of Presentation
      北海道旭川市
    • Related Report
      2011 Research-status Report
  • [Presentation] キンギョ嗅球におけるドーパミンによる神経修飾作用2011

    • Author(s)
      河合喬文、阿部秀樹、岡良隆
    • Organizer
      第82回日本動物学会大会
    • Place of Presentation
      北海道旭川市
    • Related Report
      2011 Research-status Report
  • [Presentation] ドワーフグラミー終神経GnRHニューロン細胞塊におけるGnRHニューロン間結合様式2011

    • Author(s)
      阿部秀樹、西川穂高、岡良隆
    • Organizer
      第82回日本動物学会大会
    • Place of Presentation
      北海道旭川市
    • Related Report
      2011 Research-status Report
  • [Presentation] トランスジェニックメダカを用いたkiss1ニューロンの電気生理学的解析2011

    • Author(s)
      島田洋之、神田真司、赤染康久、大久保範聡、阿部秀樹、岡良隆
    • Organizer
      第82回日本動物学会大会
    • Place of Presentation
      北海道旭川市
    • Related Report
      2011 Research-status Report
  • [Presentation] Functional role of GnRH receptors for neuromodulation in the goldfish olfactory bulb2011

    • Author(s)
      Kawai, T., Kanda, S., Akazome, Y., Abe, H., and Oka, Y.
    • Organizer
      41st Annual Meeting, Society for Neuroscience
    • Place of Presentation
      Washington DC, USA
    • Related Report
      2011 Research-status Report
  • [Presentation] Analysis of regulatory mechanisms of gonadotropin secretion using transgenic medaka (Oryzias latipes)2011

    • Author(s)
      Karigo, T., Aikawa, M., Kanda, S., Abe H., Akazome,Y. Okubo, K., and Oka, Y.
    • Organizer
      41st Annual Meeting, Society for Neuroscience
    • Place of Presentation
      Washington DC, USA
    • Related Report
      2011 Research-status Report
  • [Presentation] Analysis of the molecular and neural mechanism of female mating acceptance depending on visual information in small fish medaka2011

    • Author(s)
      Okuyama, T., Suehiro, Y., Imada, H., Shimada, A., Abe, H., Tanaka, M., Naruse, K., Takeda, H., Oka, Y., Kubo, Y. and Takeuchi, H.
    • Organizer
      41st Annual Meeting, Society for Neuroscience
    • Place of Presentation
      Washington DC, USA
    • Related Report
      2011 Research-status Report
  • [Presentation] Analysis of the molecular and neural mechanism of female sexual preference that depends on visual information in small fish medaka2011

    • Author(s)
      Okuyama T., Abe, H., Suehiro, Y., Imada, H., Shimada, A., Kawasaki, T., Yuba, S., Taniguchi, Y., Kamei, Y., Tanaka, M., Naruse, N., Takeda, H., Oka, Y., Kubo, T., Takeuchi, H.
    • Organizer
      第17回小型魚類研究会
    • Place of Presentation
      静岡県三島市
    • Related Report
      2011 Research-status Report
  • [Presentation] Analysis of the molecular and neural mechanism of female mating acceptance depending on visual information in small fish medaka2011

    • Author(s)
      Teruhiro Okuyama, Yuji Suehiro, Haruka Imada, Atsuko Shimada, Hideki Abe, Minoru Tanaka, Kiyoshi Naruse, Hiroyuki Takeda, Yoshitaka Oka, Takeo Kubo, Hideaki Takeuchi
    • Organizer
      1st medaka strategic meeting
    • Place of Presentation
      愛知県岡崎市
    • Related Report
      2011 Research-status Report
  • [Presentation] 小型魚類を用いた視覚情報依存的な配偶者選択行動の分子・神経基盤解析2011

    • Author(s)
      奥山輝大, 阿部秀樹, 末廣勇司, 今田はるか, 島田敦子, 川崎隆史, 弓場俊輔, 谷口善仁, 亀井保博, 田中実, 成瀬清, 武田洋幸, 岡良隆, 久保健雄, 竹内秀明
    • Organizer
      第34回 日本分子生物学会年会
    • Place of Presentation
      神奈川県横浜市
    • Related Report
      2011 Research-status Report
  • [Presentation] 遺伝子改変メダカを用いた黄体形成ホルモン(LH)放出の中枢制御機構の解析

    • Author(s)
      近藤千香,苅郷友美,相川雅人,神田真司,赤染康久,大久保範聡,阿部秀樹,岡良隆
    • Organizer
      日本動物学会関東支部第65会大会
    • Place of Presentation
      東京工業大学
    • Related Report
      2012 Annual Research Report
  • [Presentation] 終神経GnRHニューロンにおけるバースト活動の誘起

    • Author(s)
      河合喬文、阿部秀樹、岡良隆
    • Organizer
      第105回近畿生理学談話会
    • Place of Presentation
      関西医科大学
    • Related Report
      2012 Annual Research Report
  • [Remarks]

    • URL

      http://www.agr.nagoya-u.ac.jp/~hikaku/

    • Related Report
      2012 Final Research Report
  • [Remarks]

    • URL

      http://hidekiabe.web.fc2.com/

    • Related Report
      2012 Final Research Report
  • [Remarks]

    • URL

      http://researchmap.jp/habe/

    • Related Report
      2012 Final Research Report
  • [Remarks] 阿部秀樹 個人ページ

    • URL

      http://hidekiabe.web.fc2.com/index.html

    • Related Report
      2012 Annual Research Report
  • [Remarks] ReaD&Researchmapにおける紹介ページ

    • URL

      http://researchmap.jp/habe/

    • Related Report
      2012 Annual Research Report
  • [Remarks] 名古屋大学大学院生命農学研究科水圏動物学研究分野

    • URL

      http://www.agr.nagoya-u.ac.jp/~hikaku/

    • Related Report
      2012 Annual Research Report

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Published: 2011-08-05   Modified: 2019-07-29  

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