| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Outline of Final Research Achievements |
The growth cone is a highly motile structure in the growing tip of a neurite at the stages of the neuronal development and regeneration. To visualize the vesicular trafficking and actin reorganization of the growth cone in detail simultaneously, we analyzed the dynamics of GFP-synaptophysin and mCherry-actin in the growth cone of NG108-15 cells, using a superresolution microscopy. We found that the vesicles arose near the root portions of filopodia, and most of them were retrogradely moving along the actin bundles. Both of such events were inhibited by actin polymerization inhibitors or a myosin light-chain kinase inhibitor. RNAi of fascin also decreased the numbers of vesicles localized at the leading edge. Moreover, the retrogradely moving vesicles were not colocalized with clathrin but with endophilin and dynamin. These results suggest that there is a novel mechanism of membrane retrieval in the growth cone.
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