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Application of artificial antibody for an inhibitor against signal transduction

Research Project

Project/Area Number 24760653
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Biofunction/Bioprocess
Research InstitutionHyogo University of Health Sciences

Principal Investigator

SHIBASAKI Seiji  兵庫医療大学, 共通教育センター, 准教授 (50342530)

Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywords人工抗体 / シグナル伝達 / 滑膜細胞 / 筋芽細胞 / アフィボディー / PCA
Research Abstract

In this study, protein library using yeast PCA method has been developed for screening system of artificial antibody. Thus, the screening method of cytosolic target-affinity molecules was established. Important molecules of intracellular signal transduction cascade, Ras and Raf, were chosen as target molecules. Then, affinity molecules against these molecules were selected and evaluated in cytosol. As a model disease, rheumatoid arthritis which is involved in Ras/Raf signal transduction cascade. Under these conditions, selected affibody clones were evaluated on proliferation or cytokine-production using synovial and osteoblast cell lines.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (17 results)

All 2014 2013 2012 Other

All Journal Article (6 results) (of which Peer Reviewed: 6 results) Presentation (11 results)

  • [Journal Article] Evaluation of Mdh1 Protein as an Antigenic Candidate for a Vaccine against Candidiasis2014

    • Author(s)
      Shibasaki S, Aoki W, Nomura T, Karasaki M, Sewaki T, Ueda M.
    • Journal Title

      Biocontrol Sci

      Volume: 19(1) Pages: 51-51

    • NAID

      130003395463

    • URL

      https://www.jstage.jst.go.jp/article/bio/19/1/19_51/_article

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] An oral vaccine against candidiasis generated by a yeast molecular display system2013

    • Author(s)
      Shibasaki S, Aoki W, Nomura T, Miyoshi A, Tafuku S, Sewaki T, Ueda M.
    • Journal Title

      Pathog Dis

      Volume: 69(3) Issue: 3 Pages: 262-262

    • DOI

      10.1111/2049-632x.12068

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Biochemical Analysis and Application of Molecular Display Technology on Candida albicans for Diagnosing and Preventing Candidiasis2013

    • Author(s)
      Shibasaki S, Aoki W, Ueda M.
    • Journal Title

      Yakugaku Zasshi

      Volume: 133 Pages: 1145-1145

    • NAID

      130003382266

    • URL

      http://www.jstage.jst.go.jp/article/yakushi/133/11/133_13-00212-1/_article

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Hepatocyte growth factor regulates immune reactions caused by transplantation and autoimmune diseases2013

    • Author(s)
      Iwasaki T, Shibasaki S.
    • Journal Title

      Yakugaku Zasshi

      Volume: 133 Pages: 1159-1159

    • NAID

      130003382268

    • URL

      http://www.jstage.jst.go.jp/article/yakushi/133/11/133_13-00212-3/_article

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Detection of Candida albicans by using a designed fluorescence-quenched peptide2013

    • Author(s)
      Aoki W, Kitahara N, Fujita A, Shibasaki S, Morisaka H, Kuroda K, Ueda M.
    • Journal Title

      J Biosci Bioeng

      Volume: 116(5) Issue: 5 Pages: 573-573

    • DOI

      10.1016/j.jbiosc.2013.05.003

    • NAID

      110009685206

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Effect of transient expression of fluorescent protein probes in synovial and myoblast cell lines2012

    • Author(s)
      Shibasaki S, Fujita A, Usui C, Watanabe S, Kitano S, Sano H, Iwasaki T.
    • Journal Title

      Springerplus

      Volume: 1(1) Issue: 1 Pages: 36-36

    • DOI

      10.1186/2193-1801-1-36

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Presentation] 人工抗体Affibodyによる炎症性メディエーター産生の制御2014

    • Author(s)
      芝崎誠司、関口昌弘、北野幸恵、岩崎剛、佐野統
    • Organizer
      第58回日本リウマチ学会総会•学術集会
    • Place of Presentation
      グランドプリンスホテル新高輪国際館パミール(東京都)
    • Year and Date
      2014-04-25
    • Related Report
      2013 Final Research Report
  • [Presentation] 人工抗体Affibodyによる細胞内シグナル伝達系と炎症性メディエーター産生の制御2013

    • Author(s)
      芝崎誠司、唐崎美樹、関口昌弘、渡辺沙知子、岩崎剛、佐野統
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸国際展示場(神戸市)
    • Year and Date
      2013-12-05
    • Related Report
      2013 Final Research Report
  • [Presentation] 抗Ras人工抗体Affibodyの取得と細胞内シグナル伝達経路の阻害2013

    • Author(s)
      臼井千裕、唐崎美樹、関口昌弘、北野幸恵、佐野統、岩崎剛、芝崎誠司
    • Organizer
      日本薬学会第133年会
    • Place of Presentation
      パシフィコ横浜(横浜市)
    • Year and Date
      2013-03-29
    • Related Report
      2013 Final Research Report
  • [Presentation] バイオディフェンス―免疫システム分子制御の新たな局面2013

    • Author(s)
      芝崎誠司、青木航、植田充美
    • Organizer
      日本薬学会第133年会
    • Place of Presentation
      パシフィコ横浜(横浜市)
    • Year and Date
      2013-03-29
    • Related Report
      2013 Final Research Report
  • [Presentation] 抗Ras人工抗体Affibodyの取得と細胞内シグナル伝達経路の阻害2013

    • Author(s)
      臼井千裕、唐崎美樹、関口昌弘、北野幸恵、佐野統、岩崎剛、芝崎誠司
    • Organizer
      日本薬学会第133年会
    • Place of Presentation
      パシフィコ横浜(横浜)
    • Related Report
      2012 Research-status Report
  • [Presentation] 滑膜細胞における分子標的薬ならびに人工抗体リガンドを用いたサイトカイン抑制効果2012

    • Author(s)
      渡邊沙知子、唐崎美樹、関口昌弘、北野幸恵、佐野統、岩崎剛、芝崎誠司
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡国際会議場(福岡市)
    • Year and Date
      2012-12-14
    • Related Report
      2013 Final Research Report
  • [Presentation] 第三世代抗体医薬開発を目指した先導的研究2012

    • Author(s)
      芝崎誠司、東岸任弘
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡国際会議場(福岡市)
    • Year and Date
      2012-12-14
    • Related Report
      2013 Final Research Report
  • [Presentation] Evaluation of proteases derived from Candida albicans using expression system in Pichia pastoris2012

    • Author(s)
      Shibasaki S., Aoki W., Ueda M.
    • Organizer
      Japan-Finland Biotechnology Symposium 2012
    • Place of Presentation
      東北学院大学押川記念ホール(仙台市)
    • Year and Date
      2012-06-06
    • Related Report
      2013 Final Research Report
  • [Presentation] 滑膜細胞における分子標的薬ならびに人工抗体リガンドを用いたサイトカイン抑制効果2012

    • Author(s)
      渡邊沙知子、唐崎美樹、関口昌弘、北野幸恵、佐野統、岩崎剛、芝崎誠司
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡国際会議場(福岡)
    • Related Report
      2012 Research-status Report
  • [Presentation] 人工抗体Affibodyによる細胞内シグナル伝達系と炎症性メディエーター産生の制御

    • Author(s)
      芝崎誠司、唐崎美樹、関口昌弘、渡邉沙知子、岩崎剛、佐野統
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸国際展示場(神戸市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 人工抗体Affibodyによる炎症性メディエーター産生の制御

    • Author(s)
      芝崎誠司、関口昌弘、北野幸恵、岩崎剛、佐野統
    • Organizer
      第58回 日本リウマチ学会 総会・学術集会
    • Place of Presentation
      グランドプリンスホテル新高輪 国際館パミール(東京都)
    • Related Report
      2013 Annual Research Report

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Published: 2013-05-31   Modified: 2019-07-29  

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