An attempt to produce a transgenic medaka that express a cilia-related gene in a spatiotemporally regulated manner
| Project/Area Number |
25670098
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
General anatomy (including histology/embryology)
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 発生 / 繊毛 / 嚢胞腎 / 遺伝子発現制御 |
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| Outline of Final Research Achievements |
I have attempted to produce a transgenic medaka that express a cilia-related gene in a spatiotemporally regulated manner by combination of Transcription Activator-Like Effector Nuclease (TALEN) method, Cre/loxP system and heat-shock promoter. I have already achieved that a TALEN knockout medaka in which a cilia-related gene in knocked out. This fish line developed a polycystic kidney as expected. Cystic dilation was already detected at hatching and gradually increased in size as fish grew. This phenotype resembles that of human polycystic kidney disease. Construction of a transgenic line using kidney specific promoter in progress.
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Report
(3 results)
Research Products
(3 results)
