KAKEN — Research Projects | Researcher Number: 80200848

Search Results: 7 results / Researcher Number: 80200848

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1. Distribution and Functional analysis of the Cnm homologue in mutans streptococci

Research Project

Research Category	Grant-in-Aid for Scientific Research (C)
Research Field	Morphological basic dentistry
Research Institution	Tokyo Dental College
Principal Investigator	YAMAMOTO Yasuhito Tokyo Dental College
Project Period (FY)	2006 – 2008Completed
Keywords	口腔細菌 / 遺伝子 / タンパク質 / 発現制御 / 感染症 / 細菌性心内膜炎 / mutans streptococci / WAP(Wall-anchoring protein) / 細菌 / 細菌性心内膜 / WAP(Wall-anchoring Protein)
Research Abstract	グラム陽性菌のコラーゲン結合アドヘシンには、これらでよく保存さえている基本構造があり、CBD(Collagen-binding domain)を含むAドメインと、それに続く繰り返し配列からなるBリピート領域がそれである。S. ratti 107P株よりクローニングしたcnmホモログは、S. muta ...

Research Products of this project	Presentation (1 results)

2. Regulatory mechanism to avoid or induce apoptosis in lymphocytes by AMP-activated protein kinase

Research Project

Research Category	Grant-in-Aid for Scientific Research (C)
Research Field	Functional basic dentistry
Research Institution	Tokyo Dental College
Principal Investigator	KIZAKI Harutoshi Tokyo Dental College, Department of Dentistry, professor
Project Period (FY)	2003 – 2005Completed
Keywords	AMP-activated protein kinase / apoptosis / lymphocyte / glucocorticoid / thymus atrophy / masseter muscle / protein kinase C / starvation
Research Abstract	T lymphocyte dynamics in inflammatory lesions are regulated by proliferation and apoptosis of the cells under various stresses. To elucidate the molec ...

Research Products of this project	Journal Article (8 results) Publications (2 results)

3. S.mutansのピルビン酸代謝(酸産生経路)調節機構の遺伝子発現レベルでの解析

Research Project

Research Category	Grant-in-Aid for Encouragement of Young Scientists (A)
Research Field	Morphological basic dentistry
Research Institution	Tokyo Dental College
Principal Investigator	山本康人東京歯科大学, 歯学部, 助手
Project Period (FY)	1999 – 2000Completed
Keywords	Streptococcus mutans / Pyruvate formate-lyase / PFL activase / Pyruvate metabolism / streptococcus mutans
Research Abstract	申請者は,種々の発酵条件下(好気,微嫌気および高度嫌気条件下各種糖類の種々濃度)におけるS.mutansのpflとactの発現状況を,好気的な実験環境下で容易に検出できるようにするため,pfl,actそれぞれとレポーター遺伝子(cat:クロラムフェニコールアセチルトランスフェラーゼ遺伝子)との融合遺 ...

Research Products of this project	Publications (1 results)

4. S.mutansピルビン酸ギ酸リアーゼ(PFL)の発現機構の解析

Research Project

Research Category	Grant-in-Aid for Encouragement of Young Scientists (A)
Research Field	Functional basic dentistry
Research Institution	Tokyo Dental College
Principal Investigator	山本康人東京歯科大学, 歯学部, 助手
Project Period (FY)	1997 – 1998Completed
Keywords	Streptococcus mutans / Pyruvate formate-lyase / PFL activase / sugar alcohol metabolism / PFL activeting enzyme
Research Abstract	嫌気的条件下のS.mutans糖代謝系で,重要な役割を担っているビルビン酸ギ酸リアーゼ(PFL)はラジカル酵素であり,その活性化にはラジカル形成に関与するPFL活性化酵素(PFLactivase)の存在が必須である.S.mutansのDPFL活性化酵素遺伝子(act)は,E.coli等のそれと違い, ...

5. S.mutansピルビン酸ギ酸リアーゼ(PFL)の活性化系に関与する遺伝子の検索

Research Project

Research Category	Grant-in-Aid for Encouragement of Young Scientists (A)
Research Field	Functional basic dentistry
Research Institution	Tokyo Dental College
Principal Investigator	山本康人東京歯科大学, 歯学部, 助手
Project Period (FY)	1996Completed
Keywords	Streptococcus mutans / Pyruvate formate-lyase / PFL activeting enzyme / sugar alcohol metabolism
Research Abstract	申請者はピルビン酸ギ酸リアーゼ(PFL)遺伝子を欠失したS.mutansより染色体DNAを分離し、制限酵素Sau3Alで完全分解した後、ベクターとしpVA891を用いクローンバンクを作成した.このクローンバンクでS.mutansを形質転換し、染色体DNAにランダム変異を導入した変異株を3,000株ほ ...

6. S. mutans ピルビン酸ギ酸リアーゼ遺伝子(pfl)の発現調節機構の解析

Research Project

Research Category	Grant-in-Aid for Encouragement of Young Scientists (A)
Research Field	Functional basic dentistry
Research Institution	Tokyo Dental College
Principal Investigator	山本康人東京歯科大学, 歯学部・生化学講座, 助手
Project Period (FY)	1995Completed
Keywords	Streptococcus mutans / pfl gene / Pyruvate formate-lyase / sugar alcohol metabolism
Research Abstract	S. mutansの糖代謝において、特に歯垢深部の様な高度の嫌気的条件下では、ラジカル酵素であるピルビン酸ギ酸リアーゼ(PFL)がNAD/NADHバランスに重要な役割をはたしている。申請者らはS. mutansの染色体DNAにランダム変異を導入し、大腸菌以外では未だクローニングされていなかったピルビ ...

Research Products of this project	Publications (1 results)

7. Elucidation of a unique regulatory mechanism for scrA gene expression in S.mutans

Research Project

Research Category	Grant-in-Aid for General Scientific Research (C)
Research Field	Functional basic dentistry
Research Institution	TOKYO DENTAL COLLEGE
Principal Investigator	SATO Yutaka Tokyo Dental College, Dept.of Biochemistry, Associate Professor
Project Period (FY)	1993 – 1994Completed
Keywords	S.mutans / PTS / Sugar metabolism / Glucan / Aggregation / Sorbitol / Fructokinase / Pyruvate formate lyase
Research Abstract	We have investigated regulatory mechanisms of the scrA gene encoding EnzymeII of sucrose PTS from S.mutans. Expression of the scrA gene was regulated ...

Research Products of this project	Publications (8 results)