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2013 Fiscal Year Final Research Report

Elucidating Common Mechanisms of Allogeneic Authentication: Mechanisms of Sexual Reproduction in Animals and Plants

Administrative Group

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Project AreaElucidation of Common Mechanisms for Allogeneic Authentication in Animals and Plants
Project/Area Number 21112001
Research Category

Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionNagoya University

Principal Investigator

SAWADA Hitoshi  名古屋大学, 理学(系)研究科(研究院), 教授 (60158946)

Co-Investigator(Renkei-kenkyūsha) MIYADO Kenji  国立成育医療センター, 生殖医療研究部, 室長 (60324844)
INABA Kazuo  筑波大学, 大学院生命環境科学研究科, 教授 (80221779)
OKAMOTO Takashi  首都大学東京, 大学院理工学研究科, 准教授 (50285095)
BABA Tadashi  筑波大学, 大学院生命環境科学研究科, 教授 (40165056)
IWANO Megumi  奈良先端科学技術大学院大学, 助教 (50160130)
MORI Toshiyuki  早稲田大学, 高等研究所, 助教 (00462739)
Project Period (FY) 2009-07-23 – 2014-03-31
Keywords有性生殖 / 動物 / 植物 / アロ認証 / 受精 / 膜融合 / 卵 / 精子
Research Abstract

In order to elucidate the common mechanisms of sexual reproduction in animals and plants, the following research project was carried out. First of all, in order to stimulate the collaboration within this research group, two meetings were organized, of which one was held as an international meeting entitled "International Symposium on the Mechanisms of Sexual Reproduction in Animals and Plants" by inviting many speakers from all over the world. The proceedings of this meeting will be issued from Springer. News Letter No. 6 was issued on June. Forum, a website for discussing newly published papers, was organized. A microscope for Ca imaging and LC/MS/MS were purchased and shared by all the member of this research group. In order to explain and introduce the fundamental mechanisms of sexual reproduction in animals and plants, easily understandable book entitled "Fertilization in Animals and Plants" written in Japanese will be issued in next fiscal year.

  • Research Products

    (47 results)

All 2014 2013 2012 2011 2010 2009 Other

All Journal Article (15 results) (of which Peer Reviewed: 14 results) Presentation (24 results) (of which Invited: 24 results) Book (6 results) Remarks (2 results)

  • [Journal Article] Integrative omics analysis reveals differentially distributed proteins in dimorphic euspermatozoa of the squid, Loligo bleekeri2014

    • Author(s)
      Yoshida, M.A., Yamada, L., Ochi, H., Iwata, H., Tamura-Nakano, M., Sawada H., Sauer, W.H., Ogura, A., and Hirohashi, M.
    • Journal Title

      Biochem. Biophys. Res. Commun.

      Volume: (in press)

    • DOI

      10.1016/j.bbrc.2014.04.076

    • Peer Reviewed
  • [Journal Article] 自家不和合性因子とCa^<2+>シグナル2014

    • Author(s)
      澤田均
    • Journal Title

      細胞工学

      Volume: 33 Pages: 406-408

  • [Journal Article] Self/Non-self recognition mechanisms in sexual reproduction : New insight into the self-incompatibility system shared by flowering plants and hermaphroditic animals2014

    • Author(s)
      Sawada H., Morita, M., and Iwano, M.
    • Journal Title

      Biochem. Biophys. Res. Commun.

      Volume: (in press)

    • DOI

      10.1016/j.bbrc.2014.05.099

    • Peer Reviewed
  • [Journal Article] Self-incompatibility in gamete recognition : Single self-recognition determinants and multiple, nonself-recognizing ones fuction in the same individual2013

    • Author(s)
      Harada Y., and Sawada, H.
    • Journal Title

      Mol. Reprod. Dev

      Volume: 80 Pages: 2-7

    • DOI

      10.1002/mrd.22134

    • Peer Reviewed
  • [Journal Article] Identification of novel isoforms of vitellogenin expressed in ascidian eggs2013

    • Author(s)
      Akasaka, M., Kato, K.H., Kitajima, K., and Sawada, H.
    • Journal Title

      J. Exp. Zool. (Part B, Mol. Dev. Evol.)

      Volume: 320 Pages: 118-128

    • DOI

      10.1002/jez.b.22488

    • Peer Reviewed
  • [Journal Article] Glutathione transferase theta in apical ciliary tuft regulates mechanical reception and swimming behavior of sea urchin embryos2013

    • Author(s)
      Jin, Y., Yaguchi, S., Shiba, K., Yamada, L., Yaguchi, J., Shibata, D. Sawada, H., and Inaba, K.
    • Journal Title

      Cytoskeleton

      Volume: 70(8) Pages: 453-470

    • DOI

      10.1002/cm.21127

    • Peer Reviewed
  • [Journal Article] cDNA cloning, localization and candidate binding partners of acid-extractable vitelline-coat protein Ci-v-Themis-like in the ascidian Ciona intestinalis2013

    • Author(s)
      Otsuka, K., Yamada, L., and Sawada H.
    • Journal Title

      Mol. Reprod. Dev

      Volume: 80 Pages: 840-848

    • DOI

      10.1002/mrd.22213

    • Peer Reviewed
  • [Journal Article] A surface glycoprotein indispensable for gamete fusion in the social amoeba Dictyostelium discoideum2012

    • Author(s)
      Araki, Y., Shimizu, H.D., Saeki, K., Okamoto, M., Yamada, L., Ishida, K., Sawada, H., and Urushihara, H.
    • Journal Title

      Eukaryot. Cell

      Volume: 11 Pages: 638-644

    • DOI

      10.1128/EC.00028-12

    • Peer Reviewed
  • [Journal Article] Efficient targeted mutagenesis of the chordate Ciona intestinalis genome with zinc-finger nuclease2012

    • Author(s)
      Kawai, N., Ochiai, H., Sakuma, T., Yamada, L., Sawada, H., Yamamoto, T., and Sasakura, Y.
    • Journal Title

      Dev. Growth Differ

      Volume: 54 Pages: 536-545

    • DOI

      10.1111/j.1440-169X.2012.01355.x

    • Peer Reviewed
  • [Journal Article] Self-incompatibility response induced by calcium increase in sperm of the ascidian Ciona intestinalis2012

    • Author(s)
      Saito, T., Shiba, T., Inaba, K., Yamada, L., and Sawada, H.
    • Journal Title

      Natl. Acad. Sci. U.S.A.

      Volume: 109 Pages: 4158-4162

    • DOI

      10.1073/pnas.1115086109

    • Peer Reviewed
  • [Journal Article] Identification and localization of sperm CRISP family protein CiUrabin involved in gamete interaction in the ascidian Ciona intestinalis2011

    • Author(s)
      Yamaguchi, A., Saito, T., Yamada, L., Taniguchi, H., Harada, Y., and Sawada, H.
    • Journal Title

      Mol. Reprod. Dev.

      Volume: 78 Pages: 488-497

    • DOI

      10.1002/mrd.21329

    • Peer Reviewed
  • [Journal Article] Sperm-specific C-terminal processing of the proteasome PSMA1/α6 subunit2011

    • Author(s)
      Yokota, H., Kataoka, Y., Hashii, N., Kawasaki, N., and Sawada, H.
    • Journal Title

      Biochem. Biophys. Res. Commun.

      Volume: 410 Pages: 809-915

    • DOI

      10.1016/j.bbrc.2011.06.069

    • Peer Reviewed
  • [Journal Article] Vitellogenin C-terminal fragments participate in fertilization as egg-coat binding partners of sperm trypsin-like proteases in the ascidian Halocynthia roretzi2010

    • Author(s)
      Akasaka, M., Harada, Y., and Sawada, H.
    • Journal Title

      Biochem. Biophys. Res. Commun

      Volume: 392 Pages: 479-484

    • DOI

      10.1016/j.bbrc.2010.01.006

    • Peer Reviewed
  • [Journal Article] Identification of testis-specific ubiquitin-conjugating enzyme in the ascidian Ciona intestinalis2010

    • Author(s)
      Yokota, N., Harada, Y., and Sawada, H.
    • Journal Title

      Mol. Reprod. Dev

      Volume: 77 Pages: 640-647

    • DOI

      10.1002/mrd.21198

    • Peer Reviewed
  • [Journal Article] Comprehensive egg coat proteome of the ascidian Ciona intestinalis reveals gamete recognition molecules involved in self-sterility2009

    • Author(s)
      Yamada, L., Saito, T., Taniguchi, H., Sawada, H., and Harada, Y.
    • Journal Title

      J. Biol. Chem.

      Volume: 284 Pages: 9402-9410

    • DOI

      10.1074/jbc.M809672200

    • Peer Reviewed
  • [Presentation] ホヤのアロ認識機構からみた動植物共通の生殖タクティクス2013

    • Author(s)
      澤田均
    • Organizer
      日本分子生物学会
    • Place of Presentation
      神戸
    • Year and Date
      20131203-06
    • Invited
  • [Presentation] Mechanisms of self- incompatibility in the ascidian Ciona intestinalis2013

    • Author(s)
      Sawada, H.
    • Organizer
      7^<th> Tunicate Meeting
    • Place of Presentation
      Naples
    • Year and Date
      20130722-26
    • Invited
  • [Presentation] Self-sterile mechanisms in an ascidian (primitive chordate) Ciona intestinalis : Its reproductive tactics shared with flowering plants2013

    • Author(s)
      Sawada, H.
    • Organizer
      Gordon Research Conferences "Fertilization and Activation of Development"
    • Place of Presentation
      Plymouth, U.S.A
    • Year and Date
      20130714-19
    • Invited
  • [Presentation] Ascidian reproductive tactics : From basic biology to applications2013

    • Author(s)
      Sawada, H.
    • Organizer
      4^<th> International Conference Green Technology
    • Place of Presentation
      Malang, Indonesia
    • Year and Date
      2013-11-09
    • Invited
  • [Presentation] 動物と植物の生殖のはなし2013

    • Author(s)
      澤田均
    • Organizer
      名古屋大学オープンレクチャー2013
    • Place of Presentation
      名古屋
    • Year and Date
      2013-09-23
    • Invited
  • [Presentation] Glycoproteins involved in ascidian fertilization : seperm and egg-coat glycoproteins responsible for self/nonself- recognition and gamete-recognition2013

    • Author(s)
      Sawada, H.
    • Place of Presentation
      名古屋
    • Year and Date
      2013-07-05
    • Invited
  • [Presentation] Ascidian fertilization : Allorecognition and lysin systems2012

    • Author(s)
      Sawada, H.
    • Organizer
      International Symposium on the Mechanisms of Sexual Reproduction in Animals and Plants
    • Place of Presentation
      名古屋
    • Year and Date
      20121112-16
    • Invited
  • [Presentation] カタユウレイボヤにおける自家不和合性応答2012

    • Author(s)
      澤田均
    • Organizer
      日本顕微鏡学会
    • Place of Presentation
      つくば
    • Year and Date
      20120514-16
    • Invited
  • [Presentation] ホヤって何?ホヤの受精研究に魅せられて2012

    • Author(s)
      澤田均
    • Organizer
      第25回名大カフェScience and Me
    • Place of Presentation
      名古屋
    • Year and Date
      2012-08-08
    • Invited
  • [Presentation] ホヤの受精におけるアロ認識分子とその細胞応答2011

    • Author(s)
      澤田均
    • Organizer
      第9回糖鎖科学コンソーシアムシンポジウム
    • Place of Presentation
      名古屋
    • Year and Date
      20111124-25
    • Invited
  • [Presentation] ホヤの受精における自家不和合性 : 動植物に共通するアロ認識機構とカルシウムシグナル2011

    • Author(s)
      澤田均、齋藤貴子、山口顕、柴小菊、稲葉一男、山田力志
    • Organizer
      日本植物学会
    • Place of Presentation
      東京
    • Year and Date
      20110917-19
    • Invited
  • [Presentation] 原索動物ホヤ類における自己と非自己の配偶子認識機構2011

    • Author(s)
      澤田均
    • Organizer
      日本比較免疫学会
    • Place of Presentation
      横浜
    • Year and Date
      20110821-23
    • Invited
  • [Presentation] Ubiquitin-proteasome system involved in fertilization of ascidians and sea urchins2011

    • Author(s)
      Sawada, H.
    • Organizer
      Mini-symposium: Post-translational Modification in Gametes, Embryos, and the Reproductive System "Reproduction and World's Future", 44^<th> SSR meeting
    • Place of Presentation
      Portland, Oregon, U.S.A.
    • Year and Date
      20110731-0804
    • Invited
  • [Presentation] Self-nonself-recognition mechanisms during ascidian fertilization : Allelic polymorphisms of s/v-Themis-A and s/-Themis-B and a novel allorecognition pair s/v-Themis-C in Ciona intestinalis2011

    • Author(s)
      Sawada, H.
    • Organizer
      6^<th> Tunicate Meeting
    • Place of Presentation
      Montreal
    • Year and Date
      20110703-07
    • Invited
  • [Presentation] Self-sterile mechanisms in hermaphroditic marine primitive chordates (Ascidians)2011

    • Author(s)
      Sawada, H.
    • Organizer
      BIT''s^<1st> Annual Congress of Marine Biotechnology
    • Place of Presentation
      大連
    • Year and Date
      20110425-29
    • Invited
  • [Presentation] ヤの受精機構 : 雌雄同体なのになぜ自家受精しないのか2011

    • Author(s)
      澤田均
    • Organizer
      公開講演会「動物と植物の生殖のしくみ:その不思議な世界」
    • Place of Presentation
      名古屋
    • Year and Date
      2011-06-04
    • Invited
  • [Presentation] Allorecognition mechanisms in ascidian fertilization : A preproduction strategy shared with flowering plants2010

    • Author(s)
      Sawada, H.
    • Organizer
      11^<th> International Symposium on Spermatology
    • Place of Presentation
      沖縄
    • Year and Date
      20100624-29
    • Invited
  • [Presentation] Self-incompatibility systems in fertilization of hermaphroditic proto- chordates : The reproduction mechanisms shared by animals and plants2010

    • Author(s)
      Sawada, H.
    • Organizer
      International symposium of cell-cell communication plant reproduction from pollination in fertilization
    • Place of Presentation
      奈良
    • Year and Date
      20100311-12
    • Invited
  • [Presentation] Allorecognition mechanisms during ascidian fertilization : the shared reproductive strategy shared with animals and plants2010

    • Author(s)
      Sawada, H.
    • Organizer
      日本生化学会・日本分子生物学会・合同大会
    • Place of Presentation
      神戸
    • Year and Date
      2010-12-07
    • Invited
  • [Presentation] 動植物共通のアロ認証機構とホヤにおけるアロ認識機構2010

    • Author(s)
      澤田均
    • Organizer
      日本動物学会
    • Place of Presentation
      東京
    • Year and Date
      2010-09-24
    • Invited
  • [Presentation] Allorecognition systems in protochordate fertilization2010

    • Author(s)
      Sawada, H.
    • Organizer
      International Symposium "Intercellular recognition and allogeneic authentication : Perspectives of reproduction mechanisms shared by animals and plants"
    • Place of Presentation
      名古屋
    • Year and Date
      2010-01-14
    • Invited
  • [Presentation] Allogeneic authentication mechanisms during ascidian fertilization2009

    • Author(s)
      Sawada, H.
    • Organizer
      Gordon Research Conferences "Fertilization and activation of development"
    • Place of Presentation
      Plymouth, NH, U.S.A.
    • Year and Date
      20090712-17
    • Invited
  • [Presentation] ホヤの受精におけるアロ認識機構2009

    • Author(s)
      澤田均
    • Organizer
      日本分子生物学会
    • Place of Presentation
      横浜
    • Year and Date
      2009-12-10
    • Invited
  • [Presentation] 原索動物ホヤ類におけるアロ認識機構2009

    • Author(s)
      澤田均
    • Organizer
      日本生化学会
    • Place of Presentation
      神戸
    • Year and Date
      2009-10-23
    • Invited
  • [Book] Allorecognition and lysisn systems during ascidian fertilization. In Sexual Reproduction in Animals and Plants, (H. Sawada, N. Inoue, M. Iwano (eds.))2014

    • Author(s)
      Sawada, H, Yamamoto, K., Otsuka, K., Saito, T., Yamaguchi, A., Mino, M., Akasaka, M., Harada, Y., and Yamada, L.
    • Total Pages
      231-244
    • Publisher
      Springer
  • [Book] Novel isoform of vitellogenin expressed in eggs is a binding partner of the sperm proteases, HrProacrosin and HrSpermosin, in the ascidian, Halocynthia roretzi. In Sexual Reproduction in Animals and Plants, (H.Sawada, N.Inoue, M.Iwano (eds.))2014

    • Author(s)
      Akasaka, M., Kato, K. H., Kitajima, K., Sawada, H.
    • Total Pages
      131-139
    • Publisher
      Springer
  • [Book] 動物と植物の受精機構ーその多様性と共通性、動植物の受精学2014

    • Author(s)
      澤田 均、岩野 恵
    • Total Pages
      15-31
    • Publisher
      化学同人
  • [Book] 原索動物(ホヤ)の受精、動植物の受精学2014

    • Author(s)
      澤田 均、笹倉 靖徳、山田 力志
    • Total Pages
      167-184
    • Publisher
      化学同人
  • [Book] Sperm protases and extracellular ubiquitin-proteasome system involved in fertlization of ascidians and sea urchins. In : Post-translational Protein Modification in the Reproductive System, (P.Sutovsky (ed.))2014

    • Author(s)
      Sawada, H., M. Mino, and M. Akasaka
    • Publisher
      Springer (in press)
  • [Book] Allorecogntion mechanisms in ascidian fertilization : A reproduction strategy shared with flowering plants. In Sperm Cell Research in the 21st Century : Historical Discoveries to New Horizons, (M.Morisawa (ed.))2012

    • Author(s)
      Sawada, H., Saito, T., Yamaguchi, A., Harada, Y., and Yamada, L.
    • Total Pages
      202-207
    • Publisher
      Adthree Publishing Co
  • [Remarks] ①領域: (1)ホームページ

    • URL

      http://allo-authentication.net

  • [Remarks] ②研究室: (1)ホームページ

    • URL

      http://www.bio.nagoya-u.ac.jp/~SugashimaMBL/z

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Published: 2015-06-25  

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