2019 Fiscal Year Final Research Report
temperature sensing of cell cyctoplasm and cell nucleus
| Project Area | Integrative understanding of biological phenomena with temperature as a key theme |
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| Project/Area Number |
15H05929
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| Research Category |
Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
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| Allocation Type | Single-year Grants |
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| Review Section |
Biological Sciences
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| Research Institution | Institute of Physical and Chemical Research |
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Principal Investigator |
Imamoto Naoko 国立研究開発法人理化学研究所, 開拓研究本部, 主任研究員 (20202145)
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| Project Period (FY) |
2015-06-29 – 2020-03-31
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| Keywords | 核ー細胞質間輸送 / 熱ストレス / Hikeshi / Importin / 温度制御実験 / 熱ショックタンパク質Hsp70 |
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| Outline of Final Research Achievements |
The most important our research result is development of precise temperature shift assay, and finding that individual transport pathways have different sensitivities to a rise in temperature. Hikeshi mediated nuclear transport of Hsp70 occur at much lower temperature than the inhibition of Ran dependent transport, and among Ran dependent transport pathway, importin α/β dependent import ceases at a lower temperature than other Ran-dependent transport, showing these two transport pathways are affected at fever-ranged mild heat shock condition instead of severe heat shock condition. The transport activity raises or drops in narrow temperature range, suggesting physiological importance of their regulation.
Dysfunction of Hikeshi influences various biological events irrespective of stress or normal condition, due to lack of nuclear Hsp70. We expect nuclear Hsp70 is essential for nuclear proteostasis, a process that have not been carefully investigated before.
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| Free Research Field |
細胞生物学
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| Academic Significance and Societal Importance of the Research Achievements |
熱ストレスで見つけたHikeshiは本申請者が発見した分子であり、公汎な生命機能に関与するが、それが、これまで意識されてこなかったHsp70の核内機能によるものである。生命機能を理解するために、分子の核と細胞質の分配がいかに重要であるかがわかる。申請者らが樹立した精密温度制御可能な実験系から核-細胞質間輸送が熱ストレスで多段階的に応答する。そのメカニズム解明によって遺伝子発現制御の要である核-細胞質間輸送が微妙な温度変化で活性変化し、そのことが生理的に重要であることをはじめて示すことができた。本研究は、「温度を基軸とした生命現象の統合的理解」に、新たな視座を与えることに寄与したと考える。
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