2010 Fiscal Year Final Research Report
Key molecules for the genome barrier of pollen tube guidance and double fertilization
| Project Area | Genome Barriers in Plant Reproduction |
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| Project/Area Number |
18075004
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| Research Category |
Grant-in-Aid for Scientific Research on Priority Areas
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| Allocation Type | Single-year Grants |
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| Review Section |
Biological Sciences
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| Research Institution | Nagoya University (2007-2010)
The University of Tokyo (2006) |
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Principal Investigator |
HIGASHIYAMA Tetsuya Nagoya University, 大学院・理学研究科, 教授 (00313205)
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| Project Period (FY) |
2006 – 2010
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| Keywords | 植物 / 花粉管ガイダンス / 重複受精 / 細胞間シグナリング / 顕微細胞操作 |
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| Research Abstract |
Here I aimed to study the genome barrier in pollen tube guidance and double fertilization, by using the in vitro Torenia and Arabidopsis systems and our many micromanipulation techniques. I focused on pollen tube attractants derived from the synergid cell, an ovular factor AMOR that makes pollen tubes growing through the cut style competent to be attracted to the embryo sac, and dynamics of two sperm cells and molecular mechanism for double fertilization. I discovered attractant peptide LUREs that was derived from the synergid cell, which had been searched for more than 140 years. LUREs showed rapid and drastic molecular evolution consistent with that these were critical factors for reproductive isolation. AMOR was shown to be related to sugar chains specific to ovule tissues and species. Double fertilization was directly observed for the first time in the living material, whereby we could contribute to advances of this field. Visualization of double fertilization was also useful to get various mutants defective in double fertilization, which would be a significant basis for further study of the molecular mechanism of double fertilization.
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