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2010 Fiscal Year Final Research Report

Cell sensor and its model shift

Planned Research

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Project AreaMolecular interaction and modal shift of cellular sensors
Project/Area Number 18077010
Research Category

Grant-in-Aid for Scientific Research on Priority Areas

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionNational Institute for Physiological Sciences

Principal Investigator

NABEKURA Junichi  National Institute for Physiological Sciences, 発達生理学研究系, 教授 (50237583)

Co-Investigator(Kenkyū-buntansha) TOMITA Koichi  生理学研究所, 行動代謝分子解析センター, 助教 (80314285)
TOMINAGA Makoto  岡崎統合バイオサイエンスセンター, 教授 (90260041)
OKADA Yasunobu  生理学研究所, 所長 (10025661)
MAKISHIMA Makoto  日本大学, 医学部, 教授 (70346146)
KOIZUMI Shuuichi  山梨大学, 医学部, 教授 (10280752)
Co-Investigator(Renkei-kenkyūsha) TOMINAGA Makoto  岡崎統合バイオサイエンスセンター, 教授 (90260041)
OKADA Yasunobu  生理学研究所, 所長 (10025661)
MAKISHIMA Makoto  日本大学, 医学部, 教授 (70346146)
KOIZUMI Shuuichi  山梨大学, 医学部, 教授 (10280752)
Project Period (FY) 2006 – 2010
Keywords支援活動 / セルセンサー / セルソーター / 2光子励起顕微鏡 / 遺伝子改変動物
Research Abstract

To promote each research and collaboration in the Cell-Sensor team efficiently, supporting team bought the expensive and advanced apparatus, and maintained the appropriate condition. In addition, visualization of sensor molecules and analysis of sensor function have been carried-out with a high resonant multiphoton microscopy. Also, we supported the production of gene-manipulated mice. To announce the supporting activity, the technical training course has been provided, to which more than 30 members attended. We also frequently sent the new information to all members with the news letters, which printed every 3-4 month.

  • Research Products

    (14 results)

All 2011 2010 2009 2008 2007 Other

All Journal Article (10 results) (of which Peer Reviewed: 10 results) Presentation (3 results) Remarks (1 results)

  • [Journal Article] Rapid, phase-specific and size-dependent remodeling of synapses in the adult somatosensory cortex during development of neuropathic pain.2011

    • Author(s)
      Kim SK, Nabekura J.
    • Journal Title

      Journal of Neuroscience 31

      Pages: 5477-5482

    • Peer Reviewed
  • [Journal Article] Inter-regional contribution of enhanced activity of the primary somatosensory cortex to the anterior cingulate cortex accelerates chronic pain behavior.2011

    • Author(s)
      Eto K, Wake H, Watanabe M, Ishibashi H, Noda M, Yanagawa Y, Nabekura J.
    • Journal Title

      Journal of Neuroscience 31

      Pages: 7631-7636

    • Peer Reviewed
  • [Journal Article] V2 receptor-mediated autocrine role of somatodendritic release of AVP in rat vasopressin neurons under hypo-osmotic conditions.2011

    • Author(s)
      Sato K, Numata T, Saito T, Ueta Y, Okada Y.
    • Journal Title

      Science Signaling 4(157)

      Pages: ra5

    • Peer Reviewed
  • [Journal Article] The inhibitor of 20-HETE synthesis, TS-011, improves cerebral microcirculatory autoregulation impaired by middle cerebral artery occlusion in mice.2010

    • Author(s)
      Marumo T, Eto K, Wake H, Omura T, Nabekura J.
    • Journal Title

      British Journal of Pharmacology 161

      Pages: 1391-1402

    • Peer Reviewed
  • [Journal Article] Inhibition of a protein kinase Akt1 by apoptosis signal-regulating kinase-1 (ASK1) is involved in apoptotic inhibition of regulatory volume increase.2010

    • Author(s)
      Subramanyam M, Takahashi N, Hasegawa Y, Mohri T, Okada Y.
    • Journal Title

      Journal of Biological Chemistry 285

      Pages: 6109-6117

    • Peer Reviewed
  • [Journal Article] Bradykinin-induced astrocyte-neuron signaling : glutamate release is mediated by ROS-activated volume-sensitive outwardly rectifying anion channels.2009

    • Author(s)
      Liu H-T, Akita T, Shimizu T, Sabirov RZ, Okada Y.
    • Journal Title

      Journal of Physiology (London) 587

      Pages: 2197-2209

    • Peer Reviewed
  • [Journal Article] Resting microglia directly monitor the functional state of synapses in vivo and determine the fate of ischemic terminals.2009

    • Author(s)
      Wake H, Moorhouse AJ, Jinno S, Kohsaka S, Nabekura J.
    • Journal Title

      Journal of Neuroscience 29

      Pages: 3974-3980

    • Peer Reviewed
  • [Journal Article] Clustering of neuronal K^+-Cl^- cotransporter in the lipid rafts by tyrosine phosphorylation2009

    • Author(s)
      Watanabe M, Wake H, Moorhouse A, Nabekura J.
    • Journal Title

      Journal of Biological Chemistry 284

      Pages: 27980-27988

    • Peer Reviewed
  • [Journal Article] Molecular determinants of sensitivity and conductivity of human TRPM7 to Mg^<2+> and Ca^<2+>.2008

    • Author(s)
      Numata T, Okada Y.
    • Journal Title

      Channels 2

      Pages: 283-286

    • Peer Reviewed
  • [Journal Article] Early changes in KCC2 phosphorylation in response to neuronal stress results in functional downregulation.2007

    • Author(s)
      Wake H, Watanabe M, Moorhouse AJ, Kanematsu T, Horibe S, Matsukawa N, Asai K, Ojika K, Hirata M, Nabekura J.
    • Journal Title

      Journal of Neuroscience 27

      Pages: 1642-1650

    • Peer Reviewed
  • [Presentation] Microglia directly monitor the functional state of synapses and determine the fate of damaged axon terminals : in vivo observation.2010

    • Author(s)
      Nabekura J, Wake H, Eto K, Kim S, Inada H.
    • Organizer
      7th Forum of European Neuroscience
    • Place of Presentation
      Amsterdam (invited speaker)
    • Year and Date
      2010-07-06
  • [Presentation] Roles of anion channels and disordered cell volume regulation in apoptotic and necrotic cell death : International Joint Symposium2009

    • Author(s)
      Okada Y, Shimizu T, Lee EL, Inoue H, Uramoto H, Sato K, Numata T.
    • Organizer
      Physiology of Anion Transport and Cell Volume Regulation (PAT- CVR 2009)
    • Place of Presentation
      Okazaki, Japan (invited speaker)
    • Year and Date
      20090800
  • [Presentation] Roles of volume-sensitive anion channels in cell death induction and glia-to-neuron signaling2009

    • Author(s)
      Okada Y.
    • Organizer
      XXXVI International Congress of Physiological Sciences (IUPS2009)
    • Place of Presentation
      Kyoto, Japan (invited speaker)
    • Year and Date
      20090700
  • [Remarks] ホームページ

    • URL

      http://www.nips.ac.jp/cellsensor/shien.html

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Published: 2012-02-13   Modified: 2016-04-21  

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