Molecular Mechanisms of determination of gene-expression domain in leaf development

2012 Fiscal Year Final Research Report

• Project Area: Plant regulatory systems that control developmental interactions between meristems and lateral organs
• Project/Area Number: 19060004
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: National Institute for Basic Biology
• Principal Investigator: OKADA Kiyotaka 基礎生物学研究所, 所長 (50101093)
• Co-Investigator(Kenkyū-buntansha): TATEMATSU Kiyoshi 基礎生物学研究所, 植物器官形成学研究室, 助教 (00373324)
• Co-Investigator(Renkei-kenkyūsha): WADA Takuji 宮崎大学, 農学部・IR推進機構, 研究員 (50360673) ; TOMINAGA Rumi 宮崎大学, 農学部・IR推進機構, 助教 (20373334) ; TSUGEKI Ryuji 京都大学, 理学研究科, 助教 (50303805)
• Project Period (FY): 2007 – 2012
• Keywords: 植物発生 / 側生器官 / 葉の表裏 / 向背軸決定 / マイクロRNA / 茎頂分裂組織 / シロイヌナズナ

Research Abstract

We examined genetic molecular approaches to investigate mechanisms determining the adaxial and the abaxial sides of leaves, and proposed a hypothesis which succinate semialdehyde has a role in the mechanism. Second, found that the boundary separating cells expressing either the adaxial or the abaxial side-specific genes moved as the leaf grew. We proposed a mathematical model explaining the boundary shift. Third, we examined key genes necessary for lateral growth of leaves, and found that the genes are expressed newly-identified middle domain of the developing leaves.

Research Products

• [Journal Article] The leaf adaxial-abaxial boundary and lamina growth (2013)
  • Author(s): Nakata, M. & Okada, K
  • Journal Title: Plants Volume: (in press)
  • Peer Reviewed
• [Journal Article] Pattern Dynamics in Adaxial-Abaxial Specific Gene Expression is Modulated by Plastid Retrograde Signal during Arabidopsis leaf development (2013)
  • Author(s): Tameshige, T., Fujita, H., Watanabe, K., Toyokura, K., Kondo, M., Tatematsu, K., Matsumoto, N., Tsugeki, R., Kawaguchi, M., Nishimura, M., Okada. K
  • Journal Title: Plos Genetics Volume: (in press)
  • Peer Reviewed
• [Journal Article] Physical interaction of floral organs controls petal morphogenesis in Arabidopsis thaliana (2013)
  • Author(s): Takeda, S., Iwasaki, A., Matsumoto, N., Uemura, T., Tatematsu, K., Okada, K
  • Journal Title: Plant Physiol Volume: 161 Pages: 1242-1250
  • Peer Reviewed
• [Journal Article] Roles of the middle domain-specific WUSCHEL-RELATED HOMEOBOX genes in early development of leaves in Arabidopsis (2012)
  • Author(s): Nakata, M., Matsumoto, N., Tsugeki, R., Rikirsch, E., Laux, T., Okada, K
  • Journal Title: Plant Cell Volume: 24 Pages: 519-535
  • Peer Reviewed
• [Journal Article] Succinic Semialdehyde Dehydrogenase is Involved in the Robust Patterning of Arabidopsis Leaves along the Adaxial-Abaxial Axis (2011)
  • Author(s): Toyokura, K., Watanabe, K., Oiwaka, A., Kusano, M., Tameshige, T., Tatematsu, K., Matsumoto, N., Tsugeki, R., Saito, K., Okada, K
  • Journal Title: Plant & Cell Physiology Volume: 52 Pages: 1340-1353
  • Peer Reviewed
• [Journal Article] Jasmonic acid control of GLABA3 links inducible defense and trichome patterning in Arabidopsis (2009)
  • Author(s): Yoshida, Y., Sano, R., Wada, T., Takabayashi, J., Okada, K
  • Journal Title: Development Volume: 136 Pages: 1039-1048
  • Peer Reviewed
• [Journal Article] The Novel Nuclear Factor NO VEIN Mediates Auxin-Dependent Specification and Patterning in the Embryo, Shoot and Root (2009)
  • Author(s): Tsugeki, R., Ditengou, F. A., Sumi, Y., Palme, K., Okada. K
  • Journal Title: Plant Cell Volume: 21 Pages: 3133-3151
  • Peer Reviewed
• [Journal Article] Armadillo repeat-containing kinesins and a NIMA-related kinase are required for epidermal cell morphogenesis in Arabidopsis (2008)
  • Author(s): Sakai, T., van der Honing, H., Nishioka, M., Uehara, Y., Takahashi, M., Fujisawa, N., Saji, K., Seki, M., Shinozaki, K., Jones, M., Smirnoff, N., Okada, K. and Wasteneys, G
  • Journal Title: Plant J Volume: 53 Pages: 157-171
  • Peer Reviewed
• [Presentation] The abaxial-side specific expression of MIR165/166 clearly marks off the PHB-expression domain from the FIL-expression domain in Arabidopsis leaf primordia (2009)
  • Author(s): Tatematsu, K., Watanabe, K., Toyokura, K., Tameshige, T., and Okada, K
  • Organizer: The 20^<th> International Conference on Arabidopsis Research
  • Place of Presentation: Edinburgh, Scotland
  • Year and Date: 20090630-0704
• [Presentation] Region-specific expresson of regulatory genes in the lateral organ formation (2008)
  • Author(s): Okada, K
  • Organizer: SigNet International Symposium 2008
  • Place of Presentation: Korea University
  • Year and Date: 20080220-21
• [Presentation] Axis-dependent gene expression in the lateral organ formation (2007)
  • Author(s): Okada, K
  • Organizer: The 18^<th> International Conference on Arabidopsis Research
  • Place of Presentation: Beijing, China
  • Year and Date: 20070620-23