Regulatory mechanisms involved in the transition from embryonic meristem to egetative meristem

2013 Fiscal Year Final Research Report

• Project Area: Plant regulatory systems that control developmental interactions between meristems and lateral organs
• Project/Area Number: 19060011
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Chubu University (2010-2013); Nagoya University (2007-2009)
• Principal Investigator: NAKAMURA Kenzo 中部大学, 応用生物学部, 教授 (80164292)
• Co-Investigator(Kenkyū-buntansha): MORIKAMI Atsushi 名城大学, 農学部, 教授 (10211608) ; MAEO Kenichiro 名古屋大学, 大学院・生命農学研究科, 助教 (00343210)
• Project Period (FY): 2007 – 2013
• Keywords: 胚発生 / メリステム転換 / 種子発芽 / クロマチン制御 / ゲノム維持

Research Abstract

Two B3 domain repressors, HSI2 and HSL1, are essential for the transition of dormant embryonic meristem into vegetative meristem. HSI2 directly represses the expression of seed maturation genes containing RY motif and it is essential for the chromatin histone modification with the H3K27me3 inactive mark. Plants with LUC reporter genes were used for the real-time live monitoring of the repression of seed maturation genes during the early stage of germination and for the large scale screening of mutants defective in the repression of seed maturation gene after germination.

Research Products

• [Journal Article] A duplicated NUCLEOLIN gene in Arabidopsis (AtNUC2) is required for rDNA methylation and organization of silent 45S rRNA genes (2014)
  • Author(s): Durut, N., Abou-Ellail, M., Pontvianne, F., Comella, P., Chandra Das., S, Kojima, H., Ukai, S., DeBures, A., Echeverria, M., Bouvet, P., Nakamura, K. and Saez-Vasquez, J
  • Journal Title: Plant Cell in press
  • Peer Reviewed
• [Journal Article] AtNUFIP, an essential protein for plant development, reveals the impact of snoRNA gene organisation on the assembly of snoRNPs and rRNA methylation in Arabidopsis thaliana (2011)
  • Author(s): Rodor, J., Jobet, E., Bizarro, J., Vignols, F., Carles, C., Suzuki, T., Nakamura, K. and Echeverria, M
  • Journal Title: Plant J Volume: 65 Pages: 807-819
  • Peer Reviewed
• [Journal Article] Arabidopsis AUXIN RESPONSE FACTOR6 and 8 regulate jasmonic acid biosynthesis and floral organ development via repression of class 1 KNOX genes (2010)
  • Author(s): Tabata, R., Ikezaki, M., Fujibe, T., Aida, M., Tian, C.-E., Ueno, Y., Yamamoto, K.T., Machida, Y., Nakamura, K. and Ishiguro, S
  • Journal Title: Plant Cell Physiol Volume: 51 Pages: 164-175
  • Peer Reviewed
• [Journal Article] A link between DNA replication, recombination, and gene expression revealed by genetic and genomic analysis of TEBICHI gene of Arabidopsis thaliana (2009)
  • Author(s): Inagaki, S., Nakamura, K. and Morikami, A
  • Journal Title: PLoS Genet Volume: 5 Pages: e1000613
  • Peer Reviewed
• [Journal Article] An AP2-type transcription factor WRINKLED1 of Arabidopsis thaliana binds to AW-box sequence conserved among proximal upstream regions of genes involved in fatty acid synthesis (2009)
  • Author(s): Maeo, K., Tokuda, T., Ayame, A., Mitsui, N., Kawai, T., Tsukagoshi, H., Ishiguro, S. and Nakamura, K
  • Journal Title: Plant J Volume: 60 Pages: 476-487
  • Peer Reviewed
• [Journal Article] An oncoprotein from the plant pathogen Agrobacterium has histone-chaperone-like activity (2007)
  • Author(s): Terakura, S., Tagami, H., Kitakura, S., Ueno, Y., Machida, C., Wabiko, H., Aiba, H., Otten, L., Tsukagoshi, H., Nakamura, K. and Machida, Y
  • Journal Title: Plant Cell Volume: 19 Pages: 2855-2865
  • Peer Reviewed
• [Journal Article] Two B3 domain transcriptional repressors prevent sugar-inducible expression of seed maturation genes in Arabidopsis seedlings (2007)
  • Author(s): Tsukagoshi, H., Morikami, A. and Nakamura, K
  • Journal Title: Proc. Natl. Acad. Sci. USA Volume: 104 Pages: 2543-2547
  • Peer Reviewed
• [Patent(Industrial Property Rights)] 植物の油脂生産性を増大させる遺伝子及びその利用方法。 (2011)
  • Inventor(s): 中村研三、河合都妙、前尾健一郎、松本貴幸、伊藤節嗣、松田雅俊
  • Industrial Property Rights Holder: 名古屋大学、トヨタ自動車
  • Industrial Property Number: 特許, 特願 2011-053361
  • Filing Date: 2011-03-10
• [Patent(Industrial Property Rights)] 植物の油脂生産性を増大させる遺伝子及びその利用方法。 (2010)
  • Inventor(s): 中村研三、河合都妙、橋本実佳、小内清、石浦正寛、松田雅俊
  • Industrial Property Rights Holder: 名古屋大学
  • Industrial Property Number: 特許, 特願 2010-054484
  • Filing Date: 2010-03-11