Epigenetic control of meristem functions.

2009 Fiscal Year Self-evaluation Report

• Project Area: Plant regulatory systems that control developmental interactions between meristems and lateral organs
• Project/Area Number: 19060014
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: National Institute of Genetics
• Principal Investigator: KAKUTANI Tetsuji National Institute of Genetics, 総合遺伝研究系, 教授 (20332174)
• Project Period (FY): 2007 – 2012
• Keywords: DNAメチル化 / small RNA / ヒストン修飾

Research Abstract

遺伝子発現情報が塩基配列以外の形(染色体蛋白質やDNAの修飾)で細胞分裂後も染色体上に保持される「エピジェネティック」な制御は、多細胞生物の発生過程で大きな役割をもつことが明らかになりつつある。私達はこれまで、DNA低メチル化突然変異下で誘発される発生異常を連鎖解析することによってエピジェネティックな発生制御の標的となる遺伝子を同定するという独自のアプローチをとり、DNAメチル化で制御されるインプリント遺伝子など、いくつかの興味深い遺伝子を同定してきた。このようにして見いだした遺伝子の一つであるBONSAIは、メリステム機能に関与し、その抑制が葉序の異常を引き起こす。本研究では、BONSAI遺伝子など、DNAメチル化で発現が影響される遺伝子を研究素材として、これらの遺伝子における遺伝子発現を制御する機構と、個体発生に対するインパクトを研究する。

Research Products

• [Journal Article] Bursts of retrotransposition reproduced in Arabidopsis. (2009)
  • Author(s): Tsukahara S, Kobayashi A, Kawabe A, Mathieu O, Miura A, Kakutani T
  • Journal Title: Nature 461 Pages: 423-426
• [Journal Article] An Arabidopsis jmjC domain protein protects transcribed genes from DNA methylation at CHG sites. (2009)
  • Author(s): Miura A, Nakmura M, Inagaki S, Kobayashi A, Saze H, Kakutani T
  • Journal Title: EMBO J. 28 Pages: 1078-1086
• [Journal Article] Induction of RNA-directed DNA methylation upon decondensation of constitutive heterochromatin. (2009)
  • Author(s): Schoft V, Chumak N, Mosiolek M, Slusarz L, Komnenovic V, Brownfield L, Twell D, Kakutani T, Tamaru H
  • Journal Title: EMBO Rep. 10 Pages: 1078-1086
• [Journal Article] Control of Genic DNA methylation by a jmjC domain-containing protein in Arabidopsis thaliana. (2008)
  • Author(s): Saze H, Shiraishi A, Miura A, Kakutani T
  • Journal Title: Science 319 Pages: 462-465
• [Journal Article] Evolution and control of imprinted FWA genes in the genus Arabidopsis. (2008)
  • Author(s): Fujimoto R, Kinoshita Y, Kawabe A, Kinoshita T, Takashima K, Nordborg M, Nasrallah M, Shimizu K, Kudoh H, Kakutani T
  • Journal Title: PLoS Genet. 4 Pages: 1000048
• [Journal Article] Heritable epigenetic mutation of a transposon-flanked gene due to lack of the chromatin-remodeling factor DDM1. (2007)
  • Author(s): Saze H, Kakutani T
  • Journal Title: EMBO J. 26 Pages: 3641-3652
• [Presentation] Genetics of DNA methylation in genes and transposons in Arabidopsis. (2009)
  • Author(s): Kakutani T
  • Organizer: Gordon Research Conference; Epigenetics
  • Place of Presentation: Holderness, NH
  • Year and Date: 20090000
• [Presentation] Genetics of DNA methylation in Arabidopsis thaliana. (2009)
  • Author(s): Kakutani T
  • Organizer: The 24th Naito Conference on Nuclear Dynamics and RNA.
  • Place of Presentation: 札幌
  • Year and Date: 20090000
• [Presentation] Genetics of DNA methylation in genes and transposons in Arabidopsis thaliana. (2009)
  • Author(s): Kakutani T
  • Organizer: EMBL conference on Chromatin and Epigenetics.
  • Place of Presentation: Heidelberg, Germany
  • Year and Date: 20090000
• [Presentation] Epigenetic inheritance of developmental varition, transposon activity, and DNA methylation in Arabidopsis thaliana. (2008)
  • Author(s): Kakutani T
  • Organizer: The 6th NIBB-EMBL Conference; Epigenetics.
  • Place of Presentation: Heidelberg, Germany
  • Year and Date: 20080000
• [Presentation] Genetics of DNA methylation in the BONSAI locus. (2008)
  • Author(s): Kakutani T
  • Organizer: The 55th NIBB Conference; Frontier of Plant Science in the 21st Century.
  • Place of Presentation: 岡崎
  • Year and Date: 20080000
• [Presentation] Genetic dissection of DNA methylation at the BONSAI locus. (2007)
  • Author(s): Saze H, Kakutani T
  • Organizer: Gordon Research Conference; Epigenetics
  • Place of Presentation: Holderness, NH
  • Year and Date: 20070000
• [Presentation] シロイヌナズナにおけるエピジェネティックな遺伝 (2007)
  • Author(s): 角谷徹仁
  • Organizer: 日本エピジェネティクス研究会第1回年会
  • Year and Date: 20070000
• [Presentation] Epigenetic inheritance of developmental variation, transposon activity, and DNA methylation in Arabidopsis thaliana. (2007)
  • Author(s): Kakutani T
  • Organizer: 日本分子生物学第7回春期シンポジウム
  • Place of Presentation: 淡路島
  • Year and Date: 20070000
• [Book] 実験医学 (2009)
  • Author(s): 角谷徹仁、河邊昭
  • Total Pages: 3075-3079
• [Book] Epigenetics (2008)
  • Author(s): Saze H, Sasaki T, Kakutani T
  • Total Pages: 122-124
• [Book] 蛋白質核酸酵素 (2008)
  • Author(s): 佐々木卓、佐瀬英俊、角谷徹仁
  • Total Pages: 809-814
• [Book] 植物のエピジェネティクス (2008)
  • Author(s): 中村みゆき、佐瀬英俊、角谷徹仁
  • Total Pages: 57-63
• [Book] 実験医学 (2007)
  • Author(s): 角谷徹仁
  • Total Pages: 1220-1224
• [Remarks]
  • URL: http://www.nig.ac.jp/labs/AgrGen/home-j.html