High-sensitivity analysis of expression profile and subcellular dynamics of gene products in shoot apical meristem

2012 Fiscal Year Final Research Report

• Project Area: Plant regulatory systems that control developmental interactions between meristems and lateral organs
• Project/Area Number: 19060016
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Kyoto University
• Principal Investigator: ARAKI Takashi 京都大学, 大学院・生命科学研究科, 教授 (00273433)
• Co-Investigator(Kenkyū-buntansha): FUKUDA Hiroo 東京大学, 大学院・理学系研究科, 教授 (10165293) ; MACHIDA Yasunori 名古屋大学, 大学院・理学研究科, 教授 (80175596) ; FUKAKI Hidehiro 神戸大学, 大学院・理学研究科, 准教授 (80324979) ; SHIMAMOTO Ko 奈良先端科学技術大学院大学, バイオサイエンス研究科, 教授 (10263427) ; YAMAMOTO Kotaro 北海道大学, 大学院・理学研究科, 教授 (80142008)
• Project Period (FY): 2007 – 2012
• Keywords: 植物 / 生体分子 / 網羅的遺伝子解析 / 分子間相互作用 / イメージング / マイクロアレイ / タイリングアレイ / ジーンチップ

Research Abstract

Arabidopsis microarray analysis was performed in Fukuda Lab upon request from project members (total of 143 analyses from 39 laboratories). Based on these support, novel findings were obtained to advance our understanding of plant meristems, such as cross-talk of signaling pathways of peptide and plant hormones. Support for imaging by confocal laser scanning microscope and provision of BiFC vectors (15 request from 15 laboratories) resulted in important demonstration in planta of protein-protein interaction between key regulators of plant meristem function.

Research Products

• [Journal Article] Phloem long-distance delivery of FLOWERING LOCUS T (FT) to the apex (2013)
  • Author(s): Yoo, S.-C., Chen, C., Rojas, M., Daimon, Y., Ham, B.-K., Araki, T., and Lucas, W
  • Journal Title: Plant Journal Volume: Vol.74(印刷中)
  • DOI: DOI:10.1111/tpj.12213
  • Peer Reviewed
• [Journal Article] BRANCHED1 interacts with FLOWERING LOCUS T to repress the floral transition of the axillary meristems in Arabidopsis (2013)
  • Author(s): Niwa, M., Daimon, Y., Kurotani, K., Higo, A., Pruneda-Paz, J.L., Breton, G., Mitsuda, N., Kay, S.A., Ohme-Takagi, M., Endo, M., and Araki, T
  • Journal Title: Plant Cell Volume: Vol.25、No.4 Pages: 1228-1242
  • DOI: DOI:10.1105/tpc.112.109090
  • Peer Reviewed
• [Journal Article] An atypical bHLH transcription factor regulate early xylem development downstream of auxin (2013)
  • Author(s): Ohashi-Ito, K., Matsukawa, M., and Fukuda, H
  • Journal Title: Plant & Cell Physiology Volume: Vol.54、No.3 Pages: 398-405
  • Peer Reviewed
• [Journal Article] The florigen genes FT and TSF modulate lateral shoot outgrowth in Arabidopsis thaliana (2013)
  • Author(s): Hiraoka, K., Yamaguchi, A., Abe, M., and Araki, T
  • Journal Title: Plant & Cell Physiology Volume: Vol.54、No.3 Pages: 352-368
  • DOI: DOI:10.1093/pcp/pcs168
  • Peer Reviewed
• [Journal Article] Plant meristems and organogenesis: the new era of plant developmental research. (Editorial) (2013)
  • Author(s): Machida, Y., Fukaki, H., and Araki, T
  • Journal Title: Plant & Cell Physiology Volume: Vol.54、No.3 Pages: 295-301
  • DOI: DOI:10.1093/pcp/pct034
• [Journal Article] Auxin-associated initiation of vascular cell differentiation by LONESOME HIGHWAY (2013)
  • Author(s): Ohashi-Ito, K., Obuchi, M., Kojima, M., Sakakibara, H., and Fukuda, H
  • Journal Title: Development Volume: Vol.140、No.4 Pages: 765-769
  • DOI: DOI:10.1242/dev.087924
  • Peer Reviewed
• [Journal Article] The proteasome is responsible for caspase-3-like activity during xylem development (2012)
  • Author(s): Han, J., Lin, W., Oda, Y., Cui, K. M., Fukuda, H., He, X. Q
  • Journal Title: Plant Journal Volume: Vol.72、No.1 Pages: 129-141
  • DOI: DOI:10.1111/j.1365-313X.2012.05070.x
  • Peer Reviewed
• [Journal Article] Initiation of cell wall pattern by a Rho- and microtubule-driven symmetry breaking (2012)
  • Author(s): Oda, Y., Fukuda, H
  • Journal Title: Science Volume: Vol.337、No.6100 Pages: 1333-1336
  • DOI: DOI:10.1126/science.1222597
  • Peer Reviewed
• [Journal Article] CRYPTIC PRECOCIOUS/MED12 is a novel flowering regulator with multiple target steps in Arabidopsis (2012)
  • Author(s): Imura, Y., Kobayashi, Y., Yamamoto, S., Furutani, M., Tasaka, M., Abe, M., and Araki, T
  • Journal Title: Plant & Cell Physiology Volume: Vol.53、No.2 Pages: 287-303
  • DOI: DOI:10.1093/pcp/pcs002
  • Peer Reviewed
• [Journal Article] The TFL1 homologue KSN is a regulator of continuous flowering in rose and strawberry (2012)
  • Author(s): Iwata, H., Gaston, A., Remay, A., Thouroude, T., Jeauffre, J., Kawamura, K., Oyant, L.H., Araki, T., Denoyes, B., and Foucher, F
  • Journal Title: Plant Journal Volume: Vol.69、No.1 Pages: 116-125
  • DOI: DOI:10.1111/j.1365-313X.2011.04776.x
  • Peer Reviewed
• [Journal Article] Dynamics of Arabidopsis SUN proteins during mitosis and involvement in nuclear shaping (2011)
  • Author(s): Oda, Y. and Fukuda, H
  • Journal Title: Plant Journal Volume: Vol.66、No.4 Pages: 629-641
  • DOI: DOI:10.1111/j.1365-313X.2011.04523.x
  • Peer Reviewed
• [Journal Article] Wood cell-wall structure requires local 2D-microtubule disassembly by a novel plasma membrane-anchored microtubule-associated protein (2010)
  • Author(s): Oda, Y., Iida, Y., Kondo, Y. and Fukuda, H
  • Journal Title: Current Biology Volume: Vol.20、No.13 Pages: 1197-1202
  • DOI: DOI:10.1016/j.cub.2010.05.038
  • Peer Reviewed
• [Journal Article] Transcriptional changes in CiFT-introduced transgenic trifoliate orange (Poncirus trifoliata L. Raf.) (2010)
  • Author(s): Nishikawa, F., Endo, T., Shimada, H., Fujii, H., Shimizu, T., Kobayashi, Y., Araki, T., and Omura, M
  • Journal Title: Tree Physiology Volume: Vol.30、No.3 Pages: 431-439
  • DOI: DOI:10.1093/treephys/tpp122
  • Peer Reviewed
• [Journal Article] Involvement of phytosulfokine in the attenuation of stress response during the transdifferentiation of Zinnia mesophyll cells into tracheary elements (2009)
  • Author(s): Motose, H., Iwamoto, K., Endo, S., Demura, T., Sakagami, Y., Matsubayashi, Y., Moore, K. L. and Fukuda, H
  • Journal Title: Plant Physiology Volume: Vol.150、No.1 Pages: 437-447
  • DOI: DOI:10.1104/pp.109.135954
  • Peer Reviewed
• [Journal Article] Identifying new components participating in the secondary cell wall formation of vessel elements in Zinnia and Arabidopsis (2009)
  • Author(s): Endo, S., Pesquet, E., Yamaguchi, M., Tashiro, G., Sato, M., Toyooka, K., Nishikubo, N., Motose-U., M., Kubo, M., Fukuda, H., and Demura, T
  • Journal Title: Plant Cell Volume: Vol.21、No.4 Pages: 3152-3169
  • DOI: DOI:10.1105/tpc108.059154
  • Peer Reviewed
• [Journal Article] Adaptation of a seedling micro-grafting technique to the study of long-distance signaling in flowering of Arabidopsis thaliana (2009)
  • Author(s): Notaguchi, M., Daimon, Y., Abe, M., and Araki, T
  • Journal Title: Journal of Plant Research Volume: Vol.122、No.2 Pages: 201-214
  • DOI: DOI:10.1007/s10265-008-0209-1
  • Peer Reviewed
• [Journal Article] Non-cell-autonomous control of vascular stem cell fates by a CLE peptide/receptor system (2008)
  • Author(s): Hirakawa, Y., Shinohara, H., Kondo, Y., Inoue, A., Nakanomyo, I., Ogawa, M., Sawa, S., Ohashi-Ito, K., Matsubayashi, Y., Fukuda, H
  • Journal Title: Proceedings of the National Academy of Sciences of the United States of America Volume: Vol.105、No.39 Pages: 15208-15213
  • DOI: DOI:10.1073/pnas.0808444105
  • Peer Reviewed
• [Journal Article] Long-distance, graft-transmissible action of Arabidopsis FLOWERING LOCUS T protein to promote flowering (2008)
  • Author(s): Notaguchi, M., Abe, M., Kimura, T., Daimon, Y., Kobayashi, T., Yamaguchi, A., Tomita, Y., Dohi, K., Mori, M., and Araki, T
  • Journal Title: Plant & Cell Physiology Volume: Vol.49、No.11 Pages: 1645-1658
  • DOI: DOI:10.1093/pcp/pcn154
  • Peer Reviewed
• [Journal Article] TERE; a novel cis-element responsible for a coordinated expression of genes related to programmed cell death and secondary wall formation during tracheary element differentiation (2007)
  • Author(s): Pyo, H., Demura, T., and Fukuda, H
  • Journal Title: Plant Journal Volume: Vol.51、No.6 Pages: 955-965
  • DOI: DOI:10.1111/j.1365-313X.2007.03180.x
  • Peer Reviewed
• [Journal Article] 生命現象はどこまで視覚化できるか(4)BiFC (Bimolecular Fluorescence Complementation)法 (2007)
  • Author(s): 大門靖史, 荒木 崇
  • Journal Title: 化学と生物 Volume: Vol.45、No.10 Pages: 718-725
  • DOI: DOI:10.1271/kagakutoseibutsu1962.45.718
• [Presentation] 花成研究の動向と今後の展望 -モデル植物を中心に- (2012)
  • Author(s): 荒木 崇
  • Organizer: 第85回日本生化学会年会 シンポジウム「フロリゲン研究の新展開:その分子構造と新規な機能」
  • Place of Presentation: 福岡市・福岡国際会議場, マリンメッセ福岡
  • Year and Date: 20121214-16
• [Presentation] Role of LEAFY in gametophyte development and function (2012)
  • Author(s): 酒井友希・宮下結衣・宇山和樹・辻井由香・山口礼子・荒木 崇
  • Organizer: International Marchantia Workshop 2012
  • Place of Presentation: 熊本県阿蘇郡・ホテルグリーンピア南阿蘇
  • Year and Date: 20121115-17
• [Presentation] 超解像イメージングで挑む木部細胞分化の分子ダイナミクス (2012)
  • Author(s): 小田祥久・福田裕穂
  • Organizer: 日本植物学会 第76回大会
  • Place of Presentation: 姫路市・兵庫県立大学姫路書写キャンパス
  • Year and Date: 20120915-17
• [Presentation] 二次細胞壁パターンの数理解析にむけて (2012)
  • Author(s): 小田祥久・福田裕穂
  • Organizer: 日本植物学会 第76回大会
  • Place of Presentation: 姫路市・兵庫県立大学姫路書写キャンパス
  • Year and Date: 20120915-17
• [Presentation] 開始から完了まで-維管束分化の統合的理解に向けて- (2012)
  • Author(s): 伊藤(大橋)恭子・福田裕穂
  • Organizer: 日本植物学会 第76回大会
  • Place of Presentation: 姫路市・兵庫県立大学姫路書写キャンパス
  • Year and Date: 20120915-17
• [Presentation] Regulation of microtubule organization directing spatial patterning of wall assembly during xylem cell formation in Arabidopsis (2012)
  • Author(s): 福田裕穂・小田祥久
  • Organizer: Gordon Conference "Plant & Microbial Cytoskeleton"
  • Place of Presentation: Proctor Academy, Andover, New Hampshire, USA
  • Year and Date: 20120812-17
• [Presentation] Arabidopsis FT florigen in floral transition and beyond (2012)
  • Author(s): 荒木 崇
  • Organizer: 日本植物生理学会 2012年度年会 シンポジウム「Open Questions on Rhythmic Response Systems in Plants」
  • Place of Presentation: 京都市・京都産業大学
  • Year and Date: 20120316-18
• [Presentation] 植物の成長相転換における制御因子の祖先的機能の探索 (2012)
  • Author(s): 酒井友希・荒木 崇
  • Organizer: 日本植物生理学会 2012年度年会 シンポジウム「モデル植物ゼニゴケで探る陸上植物の普遍原理と多様性」
  • Place of Presentation: 京都市・京都産業大学
  • Year and Date: 20120316-18
• [Presentation] Vascular stem cell fates are controlled by a CLE peptide secreted from the stem cell niche (2012)
  • Author(s): 福田裕穂
  • Organizer: 10th TLL Life Sciences symposium "Chemical Communication"
  • Place of Presentation: Singapore
  • Year and Date: 20120130-31
• [Presentation] Vascular stem cell fates controlled by small peptides (2012)
  • Author(s): 福田裕穂・近藤侑貴・平川有宇樹
  • Organizer: The Croucher Advanced Study Institute Symposium "Frontier in Cell Biology Research", The Chinese University of Hong Kong
  • Place of Presentation: Hong Kong, China
  • Year and Date: 20120104-06
• [Presentation] 花成研究の動向と今後の展望 -モデル植物を中心に- (2011)
  • Author(s): 荒木 崇
  • Organizer: 園芸学会平成23年度秋季年会 シンポジウム「植物の花成制御機構に関する最新の研究動向と園芸作物への活用方策」
  • Place of Presentation: 岡山市・岡山大学津島キャンパス
  • Year and Date: 20110924-26
• [Presentation] MIDD1 a novel membrane-associated MAP regulating secondary wall patterns (2011)
  • Author(s): 福田裕穂
  • Organizer: 18th International Botanical Congress (IBC2011)
  • Place of Presentation: Melbourne, Australia
  • Year and Date: 20110723-30
• [Presentation] Maintenance of vascular stem cells by a peptide (2011)
  • Author(s): 福田裕穂
  • Organizer: A symposium "Recent Advances in Plant and Microbial Biology" Institute of Plant and Microbial Biology
  • Place of Presentation: Academia Sinica, Taipei, Taiwan
  • Year and Date: 2011-06-06
• [Presentation] Regulation of flowering by the FT florigen (2010)
  • Author(s): 荒木 崇
  • Organizer: The Eighth International Conference on "Plant Biology Frontiers: Cells and Signals"
  • Place of Presentation: Wuyishan, Fujian, China
  • Year and Date: 20100923-27
• [Presentation] CLE peptides govern vascular stem cell fate (2010)
  • Author(s): 福田裕穂
  • Organizer: The Eighth International Conference on "Plant Biology Frontiers: Cells and Signals"
  • Place of Presentation: Wuyishan, Fujian, China
  • Year and Date: 20100923-27
• [Presentation] BiFC法を用いた植物細胞内における蛋白質間相互作用の可視化と細胞内局在の数値化への試み (2009)
  • Author(s): 大門靖史・荒木 崇
  • Organizer: 第一回「光塾」(光イメージング若手の会)
  • Place of Presentation: 神戸市・情報通信研究機構 神戸研究所 未来ICT研究センター
  • Year and Date: 20090815-16
• [Presentation] Long-distance and local signaling in the regulation of flowering in Arabidopsis (2009)
  • Author(s): 荒木 崇
  • Organizer: Keystone Symposia on Molecular and Cellular Biology "Plant Sensing, Response and Adaptation to the Environment"
  • Place of Presentation: Big Sky, Montana, USA
  • Year and Date: 20090111-16
• [Presentation] 管状要素分化転換過程における遺伝子発現に対するDNAメチル化・ヒストン脱アセチル化阻害の影響 (2008)
  • Author(s): 貴舩永津子・岩本訓知・十時 泰・豊田 敦・持田恵一・出村 拓・福田裕穂
  • Organizer: 日本植物生理学会2008年度年会
  • Place of Presentation: 札幌市・札幌コンベンションセンター
  • Year and Date: 20080320-22
• [Presentation] Long-distance and local signaling in the regulation of flowering in Arabidopsis (2008)
  • Author(s): 荒木 崇
  • Organizer: SigNet International Symposium 2008
  • Place of Presentation: Korea University, Seoul, Korea
  • Year and Date: 20080220-21
• [Presentation] Long-distance signals of flowering in Arabidopsis (2007)
  • Author(s): 荒木 崇
  • Organizer: Plant Vascular Biology 2007
  • Place of Presentation: Academia Sinica, Taipei, Taiwan
  • Year and Date: 20070507-11
• [Book] Handbook of Biologically Active Peptides, 2nd Edition (2013)
  • Author(s): A. J. Kastin (ed.)
  • Total Pages: 71-75
  • Publisher: Elsevier
• [Book] Methods in Molecular Biology Vol.715: The Plant Cell Wall (2011)
  • Author(s): Z. A. Popper (ed.)
  • Total Pages: 1-20
  • Publisher: Springer
• [Book] 2010、植物のシグナル伝達 - 分子と応答 (2011)
  • Author(s): 柿本 辰男、高山 誠司、福田裕穂、松岡 信(編)
  • Total Pages: 172-177
  • Publisher: 共立出版
• [Book] Plant Signaling Peptides (2010)
  • Author(s): R. Irving and C. Gehring (eds.)
  • Total Pages: 59-76
  • Publisher: Springer
• [Book] 新しい植物ホルモンの科学 第2版 (2010)
  • Author(s): 神谷勇治・小柴共一 (編)
  • Total Pages: 169-182
  • Publisher: 講談社サイエンティフィク
• [Book] Encyclopedia of Life Sciences (ELS) (2010)
  • Author(s): A. M. Hetherington (ed.)
  • Publisher: Wiley
  • URL: DOI:10.1002/9780470015902.a0001814.pub2, Wiley Online Library
• [Book] 植物の科学 (2009)
  • Author(s): 塚谷裕一・荒木 崇(編)
  • Total Pages: 270
  • Publisher: 放送大学教育振興会
• [Book] 光周性の分子生物学 (2009)
  • Author(s): 海老原史樹文・井澤 毅 (編)
  • Total Pages: 53-63
  • Publisher: シュプリンガー・フェアラーク東京
• [Book] 花はなぜ咲くの?(植物まるかじり叢書3) (2008)
  • Author(s): 日本植物生理学会(監修)、荒木 崇(責任編集)、西村尚子(著)
  • Total Pages: 25-26, 60-61, 137-138, 143-149
  • Publisher: 化学同人
• [Book] 植物の生存戦略 -「じっとしているという知恵」に学ぶ - (2007)
  • Author(s): 「植物の軸と情報」特定領域研究班(編)
  • Total Pages: 3-10, 51-72, 161-183
  • Publisher: 朝日新聞社