Gene networks regulating development of primordial germ cells

2012 Fiscal Year Final Research Report

• Project Area: The germline: its developmental cycle and epigenome network
• Project/Area Number: 20062001
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Tohoku University
• Principal Investigator: MATSUI Yasuhisa 東北大学, 加齢医学研究所, 教授 (40241575)
• Co-Investigator(Kenkyū-buntansha): NOCE Toshiaki 慶應義塾大学, 先導研究センター, 特任教授 (70183902)
• Co-Investigator(Renkei-kenkyūsha): OKAMURA Daiji 東北大学, 加齢医学研究所, 助教 (80393263) ; MAEDA Ikuma 東北大学, 加齢医学研究所, 助教 (20376560)
• Project Period (FY): 2008 – 2012
• Keywords: 始原生殖細胞 / エピジェネティクス / 遺伝子改変マウス / ES細胞 / siRNA / DNAメチル化 / 転写制御 / Vasa

Research Abstract

In this research project, we have found the following three points. (1) Transcription regulators REST and Larp7 positively control survival and proliferation of primordial germ cells (PGCs), respectively, in mouse embryos. (2) The expression of PGC-specific genes is controlled by DNA demethylation of their regulatory regions. (3) Transcription regulator Max represses the expression of germ cell-specific genes in ES cells.

Research Products

• [Journal Article] Max is a repressor of germ-cell-related gene expression in mouse embryonic stem cells (2013)
  • Author(s): Maeda, I., Okamura, D., Tokitake, Y., Ikeda, M, Kawaguchi, H., Mise, N., Abe, K., Noce, T., Okuda, A, Matsui, Y
  • Journal Title: Nature Communications Volume: 4 Pages: 1754
  • DOI: DOI:10.1038/ncomms2780.
  • Peer Reviewed
• [Journal Article] High-resolution DNA methylome analysis of primordial germ cells identifies gender-specific reprogramming in mice (2013)
  • Author(s): Kobayashi, H., Sakurai, T., Miura, F., Imai, M., Mochizuki, K., Yanagisawa, E., Sakashita, A., Wakai, T., Suzuki, Y., Ito, T., Matsui ,Y., Kono, T
  • Journal Title: Genome Research Volume: 23 Pages: 616-627
  • DOI: doi:10.1101/gr.148023.112.
  • Peer Reviewed
• [Journal Article] Cell-cycle gene-specific control of transcription has a critical role in proliferation of primordial germ cells (2012)
  • Author(s): Okamura, D., Maeda, I., Taniguchi, H., Tokitake, Y., Ikeda, M., Ozato, K., Mise, N., Abe, K., Noce, T., Izpisua Belmonte, J. C. and Matsui, Y
  • Journal Title: Genes & Development Volume: 26 Pages: 2477-2482
  • DOI: Doi:10.1101/gad.202242.112
  • Peer Reviewed
• [Journal Article] REST and its downstream molecule Mek5 regulate survival of primordial germ cells (2012)
  • Author(s): Okamura, D., Mochizuki, K., Taniguchi, H., Tokitake, Y., Ikeda, M., Yamada, Y., Tournier, C., Yamaguchi, S., Tada, T., Scholer, H.R. and Matsui, Y
  • Journal Title: Developmental Biology Volume: 372 Pages: 190-202
  • URL: http://dx.doi.org/10.1016/j.ydbio.2012.09.013
  • Peer Reviewed
• [Journal Article] Interallelic and Intergenic Incompatibilities of the Prdm9 (Hst1) Gene in Mouse Hybrid Sterility (2012)
  • Author(s): Flachs, P., Mihola, O., .ime.ek, P., Gregorova, S., Schimenti, J.C., Matsui, Y., Baudat, F., de Massy, B, Pialek, J., Forejt, J, and Trachtulec, Z
  • Journal Title: PLoS Genetics Volume: 8 Pages: e1003044
  • DOI: doi:10.1371/journal.pgen.1003044
  • Peer Reviewed
• [Journal Article] Implication of DNA demethylation and bivalent histone modification for selective gene regulation in mouse primordial germ cells (2012)
  • Author(s): Mochizuki, K., Tachibana, M., Saitou, M., Tokitake,Y.,and Matsui,Y
  • Journal Title: PLoS ONE Volume: 7 Pages: e46036
  • DOI: doi:10.1371/journal.pone.0046036
  • Peer Reviewed
• [Journal Article] Chicken primordial germ cells use the anterior vitelline veins to enter the embryonic circulation (2012)
  • Author(s): Bernardo, A., Sprenkels,K. , Rodrigues, G., Noce T. and Lopes, S
  • Journal Title: Biol. Open Volume: 1 Pages: 1146-1152
  • Peer Reviewed
• [Journal Article] Induction of primordial germ cells from mouse induced pluripotent stem cells derived from adult hepatocytes (2011)
  • Author(s): Imamura, M., Aoi, T., Tokumasu, A., Mise, N., Abe, K., Yamanaka, S., and Noce, T
  • Journal Title: Molecular Reproduction and Development Volume: 77 Pages: 802-811
  • Peer Reviewed
• [Journal Article] Epigenetic profiles in primordial germ cells : global and fine tuning of the epigenome for acquisition of totipotency (2010)
  • Author(s): Mochizuki, K. and Matsui, Y
  • Journal Title: Development Growth and Differentiation Volume: 52 Pages: 517-525
  • DOI: DOI:10.1111/j.1440-169X.2010.01190.x
  • Peer Reviewed
• [Journal Article] MVH in piRNA Processing and Gene Silencing of Retrotransposons (2010)
  • Author(s): S. Kuramochi-Miyagawa, T.Watanabe, K. Gotoh, K. Takamatsu, S. Chuma, K. Kojima-Kita, Y. Shiromoto, N. Asada, T. Kimura, N. Nakatsuji, T. Noce, H. Sasaki and T. Nakano
  • Journal Title: Gene & Development Volume: 24 Pages: 887-892
  • Peer Reviewed
• [Journal Article] Primordial germ cells contain subpopulations that have greater ability to develop into pluripotential stem cells (2009)
  • Author(s): .Matsui, Y., and Tokitake, Y
  • Journal Title: Development Growth and Differentiation Volume: 51 Pages: 657-667
  • DOI: DOI:10.1111/j.1440-169X.2009.01125.x
  • Peer Reviewed
• [Journal Article] Heterogeneity of mouse primordial germ cells reflecting the distinct status of their differentiation, proliferation and apoptosis can be classified by the expression of cell surface proteins integrin a6 and c-Kit (2009)
  • Author(s): Morita-Fujimura, Y., Tokitake, Y., and Matsui, Y
  • Journal Title: Development Growth and Differentiation Volume: 51 Pages: 567-584
  • DOI: DOI:10.1111/j.1440- 169X.2009.01119.x
  • Peer Reviewed
• [Journal Article] Single Nucleotide Polymorphisms in PRDM9 (MEISETZ) in Patients with Nonobstructive Azoospermia (2009)
  • Author(s): Irie, S., Tsujimura, A., Miyagawa, Y., Ueda, T., Matsuoka, Y., Matsui, Y., Okuyama, A., Nishimune, Y., and Tanaka, H
  • Journal Title: Journal of Andorology Volume: 30 Pages: 426-431
  • DOI: DOI:10.2164/jandrol.108.006262
  • Peer Reviewed
• [Journal Article] In vitro assay system for primordial germ cell development (2009)
  • Author(s): Maeda, I. and Matsui, Y
  • Journal Title: Cell Research Volume: 19 Pages: 1125-1126
  • DOI: doi:10.1038/cr.2009
  • Peer Reviewed
• [Journal Article] Requirement of Oct3/4 for germ cell specification (2008)
  • Author(s): Okamura, D., Tokitake, Y., Niwa, H., and Matsui, Y
  • Journal Title: Developmental Biolology Volume: 317 Pages: 576-584
  • DOI: doi:10.1016/j.ydbio. 2008.03.002
  • Peer Reviewed
• [Journal Article] Epigenetic events in mammalian germ cell development : reprogramming and beyond (2008)
  • Author(s): Sasaki, H. and Matsui, Y
  • Journal Title: Nature Reviews Genetics Volume: 9 Pages: 129-140
  • DOI: doi:10.1038/nrg2295
  • Peer Reviewed
• [Journal Article] Identification of commonly expressed transcripts both in the hematopoietic and germline stem cells niche (2008)
  • Author(s): Mizukami, T, Kuramitsu, M, Takizawa, K, Momose, H, Mochizuki, M, Masumi, A, Naito, S, Iwama, A, Ogawa, T, Noce, T, Hamaguchi, I and Yamaguchi, K
  • Journal Title: Stem Cell and Development Volume: 17 Pages: 67-80
  • Peer Reviewed
• [Journal Article] Differences and similarities in the developmental status of embryo-derived stem cells and primordial germ cells revealed by global expression profiling (2008)
  • Author(s): Mise,N.,Fuchikami,T., Sugimoto,M., Kobayakawa,S., Ike,F., Ogawa,T., Tada, T., Kanaya,S., Noce, T. and Abe, K
  • Journal Title: Genes to Cells Volume: 13 Pages: 863-877
  • Peer Reviewed
• [Presentation] Selective epigenetic gene regulation via DNA demethylation and bivalent histone modification in mouse primordial germ cells (2012)
  • Author(s): K. Mochizuki, M. Tachibana, M. Saitou, Y. Tokitake and Y. Matsui
  • Organizer: Cold Spring Harbor Meeting, 'Germ Cells'
  • Place of Presentation: Cold Spring Harbor, USA
  • Year and Date: 20121002-06
• [Presentation] Max was identified as a repressor of germ-cell-related gene expression in mouse embryonic stem cells via an RNAi screen (2012)
  • Author(s): I. Maeda, Y. Tokitake, M. Ikeda, H. Kawaguchi, N. Mise, K. Abe, T. Noce, A. Okuda and Y. Matsui
  • Organizer: Cold SpringHarbor Meeting, 'Germ Cells'
  • Place of Presentation: Cold Spring Harbor, USA
  • Year and Date: 20121002-06
• [Presentation] Selective epigenetic gene regulation via DNA demethylation and bivalent histone modification in primordial germ cells (2012)
  • Author(s): K. Mochizuki, M. Tachibana, M. Saitou, Y. Tokitake and Y. Matsui
  • Organizer: Joint Meeting of The 45th Annual Meeting of the Japaneses Society of Development Biologists & The 64th Annual Meeting of the Japan Society for Cell Biology
  • Place of Presentation: Kobe
  • Year and Date: 20120528-31
• [Presentation] MaxはES細胞における生殖細胞関連遺伝子群の抑制因子である (2012)
  • Author(s): 前田郁麻、時武裕子、三〓名丹、阿部訓也、野瀬俊明、奥田昌彦、立花誠、松居靖久
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
  • Year and Date: 2012-12-12
• [Presentation] 精子形成におけるヒストン脱メチル化遺伝子Jmjd1Cの機能解析 (2012)
  • Author(s): 野瀬俊明
  • Organizer: 第35回日本分子生物学会
  • Place of Presentation: 福岡
  • Year and Date: 2012-12-12
• [Presentation] 転写制御因子Maxによる、多能性幹細胞での生殖細胞特異的遺伝子発現の抑制機構 (2012)
  • Author(s): 松居靖久、前田郁麻
  • Organizer: 文部科学省科学研究費補助金特定領域研究「生殖細胞の世代サイクルとエピゲノムネットワーク」による公開シンポジウム
  • Place of Presentation: 京都
  • Year and Date: 2012-11-21
• [Presentation] Primordial germ cells have intrinsic ability to directly acquire naive pluripotency (2012)
  • Author(s): Y. Matsui
  • Organizer: 4th Congress of the Asia Pacific Initiative on Reproduction. Symposium 2 'Stem Cell'
  • Place of Presentation: Osaka, Japan
  • Year and Date: 2012-09-01
• [Presentation] Comparative insights of mechanisms underlying germ-line establishment in mammals and in other organisms (2012)
  • Author(s): Y.Matsui
  • Organizer: 4th Congress of the Asia Pacific Initiative on Reproduction. Pre-Congress Course 1 'Investigate Commanality of Reproductive Cell going through Evolution.'
  • Place of Presentation: Osaka, Japan
  • Year and Date: 2012-08-31
• [Presentation] 多能性幹細胞と始原生殖細胞の関係と相互変換機構 (2012)
  • Author(s): 松居靖久
  • Organizer: 第30回 日本受精着床学会総会・学術講演会
  • Place of Presentation: 大阪 (招聘講演)
  • Year and Date: 2012-08-31
• [Presentation] DNA demethylation regulates primordial germ cell-specific gene expression in mouse (2011)
  • Author(s): K. Mochizuki, Y. Matsui
  • Organizer: 44th Annual Meeting of the Japanese Society of Developmental Biologists
  • Place of Presentation: Okinawa
  • Year and Date: 20110518-21
• [Presentation] Functions of an epigenetic modulator, REST for survival of primordial germ cells (2011)
  • Author(s): D. Okamura, H. Taniguchi, Y. Tokitake, Y. Yamada and Y. Matsui
  • Organizer: Keystone Symposium 'Stem Cells, Cancer and Metastasis'
  • Place of Presentation: Keystone, Colorado, USA
  • Year and Date: 20110306-11
• [Presentation] マウス始原生殖細胞の生存・増殖に関わる分子機構の解明 (2011)
  • Author(s): 岡村大治、谷口大史、時武裕子、山田泰広、松居靖久
  • Organizer: 第34回 日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-13
• [Presentation] 転写活性化因子Larp7による始原生殖細胞の増殖制御機構 (2011)
  • Author(s): 松居靖久、前田郁麻、岡村大治
  • Organizer: 文部科学省科学研究費補助金特定領域研究「生殖細胞の世代サイクルとエピゲノムネットワーク」による公開シンポジウム
  • Place of Presentation: 大阪
  • Year and Date: 2011-11-17
• [Presentation] Functions of REST and Larp7 on survival and proliferation of primordial germ cells in mouse (2011)
  • Author(s): Y. Matsui and D. Okamura
  • Organizer: Cold Spring Harbor Asia Meeting,'Developmental Control of Sex Growth and Cellular Fate
  • Place of Presentation: Shzou, China
  • Year and Date: 2011-10-12
• [Presentation] Epigenetic mechanisms underlying germ cell formation and their survival (2010)
  • Author(s): Y. Matsui, K. Mochizuki and D. Okamura
  • Organizer: International Symposium on 'Epigenome Network, Development and Reprogramming of Germ Cells'
  • Place of Presentation: Fukuoka , Japan
  • Year and Date: 20101122-24
• [Presentation] Functions of an epigenetic regulator, REST for survival of primordial germ cells (2010)
  • Author(s): D. Okamura and Y. Matsui
  • Organizer: International Symposium on 'Epigenome Network, Development and Reprogramming of Germ Cells'
  • Place of Presentation: Fukuoka, Japan
  • Year and Date: 20101122-24
• [Presentation] DNA demethylation regulates primordial germ cell-specific gene expression in mouse (2010)
  • Author(s): K. Mochizuki and Y. Matsui
  • Organizer: International Symposium on 'Epigenome Network, Development and Reprogramming of Germ Cells'
  • Place of Presentation: Fukuoka , Japan
  • Year and Date: 20101122-24
• [Presentation] DNA methylation regulates primordial germ cell-specific gene expression in mouse (2010)
  • Author(s): K. Mochizuki, Y. Matsui
  • Organizer: 43th Annual Meeting for the Japanese Society of Developmental Biologists
  • Place of Presentation: Tokushima
  • Year and Date: 20100620-23
• [Presentation] DNA demethylation regulates primordial germ cell-specific gene expression in mouse (2010)
  • Author(s): K. Mochizuki and Y. Matsui
  • Organizer: 2010 Cold Spring Harbor Asia Conference 'Epigenetics, Chromatin & Transcription'
  • Place of Presentation: Dushu Lake, China
  • Year and Date: 20100517-21
• [Presentation] マウス始原生殖細胞の分化運命決定を制御する分子メカニズム (2010)
  • Author(s): 松居靖久
  • Organizer: 第51回日本哺乳動物卵子学会、シンポジウム「生殖巣内細胞の性質とその応用」
  • Place of Presentation: 新潟
  • Year and Date: 2010-05-29
• [Presentation] 多能性幹細胞からの始原生殖細胞分化を制御する分子機構 (2010)
  • Author(s): 松居靖久
  • Organizer: 日本薬学会第130年会、シンポジウム「遺伝情報デコードによる生体調節」
  • Place of Presentation: 岡山
  • Year and Date: 2010-03-29
• [Presentation] マウスES細胞とiPS細胞からの生殖細胞の造成 (2010)
  • Author(s): 野瀬俊明
  • Organizer: 日本生殖再生医学会
  • Place of Presentation: 東京
  • Year and Date: 2010-02-21
• [Presentation] A part of undifferentiated primordial germ cells have greater ability to develop into pluripotential stem cells (2009)
  • Author(s): Y. Matsui and Y. Tokitake
  • Organizer: Cold Spring Harbor Meeting, 'Stem Cell Biology'
  • Place of Presentation: Cold Spring Harbor, USA
  • Year and Date: 20090922-26
• [Presentation] Primordial germ cells contain subpopulations that have greater ability to develop into pluripotential stem cells (2009)
  • Author(s): Y. Matsui and Y. Tokitake
  • Organizer: XXXVI International Congress of Physiological Sciences
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 20090727-0801
• [Presentation] Epigenetic regulation of primordial germ cell-specific gene expression (2009)
  • Author(s): Y. Matsui and K. Mochizuki
  • Organizer: The 24th NAITO CONFERENCE ON Nuclear Dynamics and RNA[II]
  • Place of Presentation: Sapporo, Japan
  • Year and Date: 20090623-26
• [Presentation] DNA demethylation regulates primordial germ cell-specific expression of mil-1 gene in mouse (2009)
  • Author(s): K. Mochizuki and Y. Matsui
  • Organizer: The 24th NAITO CONFERENCE ON Nuclear Dynamics and RNA[II]
  • Place of Presentation: Sapporo, Japan
  • Year and Date: 20090623-26
• [Presentation] Epigenetic control of primordial germ cell-specific gene expression (2009)
  • Author(s): Y. Matsui
  • Organizer: XX North American testis Workshop 'Testicular function : Levels of Regulation'
  • Place of Presentation: Philadelphia, USA
  • Year and Date: 20090401-04
• [Presentation] 多能性関連分子が制御するマウス始原生殖細胞の分化決定システムの解明 (2009)
  • Author(s): 岡村大治、時武裕子、谷口大史、松居靖久
  • Organizer: 文部科学省科学研究費補助金特定領域研究「生殖細胞の世代サイクルとエピゲノムネットワーク」による公開シンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2009-11-27
• [Presentation] 生殖細胞の創世技術の最近 (2009)
  • Author(s): 野瀬俊明
  • Organizer: 日本生殖医学会学術講演会
  • Place of Presentation: 金沢
  • Year and Date: 2009-11-22
• [Presentation] Heterogeneity of mouse primordial germ reflects distinct status of their differentiation, and properties of proliferation and apoptosis (2008)
  • Author(s): Y. Fujimura, Y. Tokitake and Y. Matsui
  • Organizer: XIVth International Workshop on the Development and Function of the Reproductive Organs
  • Place of Presentation: Vila Mondragone (Roma), Italia
  • Year and Date: 20080915-17
• [Presentation] Requirement of Oct3/4 function for germ cell specification (2008)
  • Author(s): D. Okamura, Y. Tokitake, H. Niwa and Y. Matsui
  • Organizer: Joint meeting of the societe Francaise de Biologie du Development /Japanese Society of Developmental Biologists, 'Frontiers in Developmental Biology'
  • Place of Presentation: Presqu'ile de Giens, France
  • Year and Date: 20080913-17
• [Presentation] DNA methylation regulates primordial germ cell-specific expression of mil-1 gene in mouse (2008)
  • Author(s): K. Mochizuki, Y. Matsui
  • Organizer: 41th Annual Meeting for the Japanese Society of Developmental Biologists
  • Place of Presentation: Tokushima
  • Year and Date: 20080528-30
• [Presentation] 始原生殖細胞が多能性幹細胞へ変化するメカニズム (2008)
  • Author(s): 松居靖久
  • Organizer: 第31回日本分子生物学会、第61回日本生化学会合同大会、シンポジウム「生殖細胞の発生を制御する分子」
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-09
• [Presentation] Potentiality of creating germ cells from ES cells (2008)
  • Author(s): T. Noce
  • Organizer: Germ cells meeting in Cold Spring Harbor Lab
  • Place of Presentation: New York, USA
  • Year and Date: 2008-10-03
• [Remarks] 研究室ホームページ
  • URL: http://www2.idac.tohoku.ac.jp/dep/crcbr
• [Remarks] 特定領域研究ホームページ
  • URL: http://ja.brc.riken.jp/lab/mcd/germline/index.html